L-myc allele polymorphism and prognosis for metastases in Russian gastric cancer patients

Mironov, Nikolai; Aguelon, Anne-Marie; Потапова, Г. И.; Gorbunov, O V; Klimenkov, A. A.; Yamasaki, Hiroshi

doi:10.1016/0959-8049(94)90606-8

Cited by 14 publications

(9 citation statements)

References 4 publications

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“…These results confirm those of Ikeda et al in colorectal cancer (1988), Tefre et al in lung cancer in Norway (1990), Ishizaki et al (1990) and Champeme et al (1992) in breast cancer, Saranath et al (1990) in oral cancer, Weston et al (1992) in lung cancer in the USA, Mironov et al (1994) in gastric cancer in Russia and Presti et al (1996) in renal cancer. On the other hand L-myc RFLP is correlated with the extent of metastasis of lung cancer in Japan (Kawashima et al, 1988(Kawashima et al, , 1992 and in renal cancer (Kakehi and Yoshida, 1989); Ishizaki et al (1990) also reported that the presence of the S-allele is associated with poor prognosis due to metastasic lesion in gastric cancer.…”

Section: Discussionsupporting

confidence: 81%

Restriction fragment length polymorphism of the L-myc gene is not a prognostic factor in bladder cancer patients

et al. 1999

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The restriction enzyme Eco RI identifies a two-allele system for the L-myc gene with fragment sizes of 10.0 and 6.6 kilobases and results in three possible allelotypes: homozygotic for the large allele (LL), homozygotic for the small allele (SS) or heterozygotic (LS). The prognostic significance of the L-myc allelotype of genomic DNA has been studied previously in several tumours (lung, colorectal, breast, soft tissue, leukaemia, lymphoma, stomach, kidney, oral cavity and liver). However, conficting results were reported. In this study we have investigated the L-myc genotype of 98 primary bladder cancer patients in an attempt to determine its utility as a prognostic, and as a susceptibility, factor in a Catalonian population. MATERIALS AND METHODSNinety-eight patients (88 males, 10 females, aged 38-91), diagnosed at Fundació Puigvert as having primary bladder cancer, were studied for L-myc restriction fragment length polymorphism (RFL); P 53 were superficial bladder tumours and 45 were invasive tumours. In addition, 110 normal DNA specimens from healthy blood donors (66 males, 44 females, aged 2-81) were analysed to investigate the genotype frequency of L-myc proto-oncogene.High molecular weight DNA was isolated by standard phenol-chloroform methods. Restriction digests of DNA were performed with endonuclease Eco RI under standard conditions, electrophoresed in a 0.8% agarose gel, denaturated, transferred to Hybond® nylon membrane (Amersham) and hybridized with a 32 P-labelled probe by the method essentially described by Southern (1975).All results obtained in these studies were analysed for statistical significance by the χ 2 test with Yates' correction for adjustment when necessary. Differences between the two populations were judged significant at P < 0.05. Kaplan-Meier curves were constructed to analyse patient survival between different genotypes (log-rank test). RESULTSEco RI-digested DNA probed with the L-myc probe results in two fragments of 10 kb (L) and 6.6 kb (S), which are due to an Eco RI restriction site polymorphism (Figure 1). The distribution of the three genotypes (LL, LS, SS) in the control and patient groups is shown in Table 1. Chi-squared analysis showed that there was no difference between the distribution of the three genotypes of patient group and controls, and all are in accordance with Hardy-Weinberg equilibrium.When the patients in the current study were examined for a relationship between the presence of S-alleles (LS or SS individuals) and distant metastasis, no association was found (P = 0.51). Nor was there a significant association between homozygosity for the Summary The L-myc restriction fragment length polymorphism has been suggested to be of prognostic significance in some types of primary tumours. We examined the prognostic and susceptibility significance of the L-myc genotype in a group of 98 bladder cancer patients. The L-myc genotype did not correlate with any pathologic parameter and does not offer any clinical utility in patients with bladder cancer.

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Section: Discussionsupporting

confidence: 81%

Restriction fragment length polymorphism of the L-myc gene is not a prognostic factor in bladder cancer patients

et al. 1999

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“…Three out of 25 rat hemangiosarcomas showed a mutation at codon 319 of Cx37 (Saito et al, 1997). But no mutation of Cx32 has been observed either in 20 human liver tumors or in 22 human stomach tumors (Krutovskikh et al, 1994;Mironov et al, 1994). Similarly, no mutation was found in Cx43 of 49 human meningiomas (Sato et al, 1997).…”

Section: Discussionmentioning

confidence: 93%

Dominant-negative abrogation of connexin-mediated cell growth control by mutant connexin genes

1997

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“…Expansion and contraction of microsatellite sequences have been reported in a discrete subset of sporadic colon cancers (2,4), in small cell lung carcinomas (17), and in tumors of the pancreas, bladder, stomach, breast, and endometrium (18)(19)(20)(21)(22)(23). However, the incidence reported for ovarian tumors is very low (=5%) (19,24,25).…”

Section: Introductionmentioning

confidence: 99%

Genetic instability in human ovarian cancer cell lines.

Orth

Hung

Gazdar

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

114

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We have analyzed the stability of microsatellites in cell lines derived from human ovarian cancers and found that 5 out of 10 of the ovarian tumor cell lines are genetically unstable at the majority of the loci analyzed. In clones and subclones derived serially from one of these cell lines (2774; serous cystadenocarcinoma), a very high proportion of microsatellites distributed in many different regions of the genome change their size in a mercurial fashion. We conclude that genomic instability in ovarian tumors is a dynamic and ongoing process whose high frequency may have been previously underestimated by PCR-based wild-type alleles of the human MSH2 gene. However the wild-type allele was lost at some point early during tumorigenesis so that DNA isolated either from the patient's ovarian tumor or from the 2774 cell line carries only the mutant allele of the human MSH2 gene. The genetic instability observed in the tumor and cell line DNA, together with the germ-line mutation in a mismatch-repair gene, suggest that the MSH2 gene is involved in the onset and/or progression in a subset of ovarian cancer.

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L-myc allele polymorphism and prognosis for metastases in Russian gastric cancer patients

Cited by 14 publications

References 4 publications

Restriction fragment length polymorphism of the L-myc gene is not a prognostic factor in bladder cancer patients

Restriction fragment length polymorphism of the L-myc gene is not a prognostic factor in bladder cancer patients

Dominant-negative abrogation of connexin-mediated cell growth control by mutant connexin genes

Genetic instability in human ovarian cancer cell lines.

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