L-DOPA Synthesis Using Tyrosinase-immobilized on Electrode Surfaces

Abstract

− Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the direct precursor of the neurotransmitter dopamine. L-DOPA is a well-known neuroprotective agent for the treatment of Parkinson's disease symptoms. L-DOPA was synthesized using the enzyme, tyrosinase, as a biocatalyst for the conversion of L-tyrosine to L-DOPA and an electrochemical method for reducing L-DOPAquinone, the product resulting from enzymatic synthesis, to L-DOPA. In this study, three electrode systems were used: A glassy carbon electrode (GCE) as working electrode, a platinum, and a Ag/ AgCl electrode as auxiliary and reference electrodes, respectively. GCE has been modified using electropolymerization of pyrrole to facilitate the electron transfer process and immobilize tyrosinase. Optimum conditions for the electropolymerization modified electrode were a temperature of 30 o C and a pH of 7 producing L-DOPA concentration 0.315 mM. After 40 days, the relative activity of an enzyme for electropolymerization remained 38.6%, respectively.

Amperometric Detection of Dopamine based on a Graphene Oxide/PEDOT:PSS Composite Electrode

“…Dopamine (DA) is one of the important neurotransmitters that plays a role in memory, hormonal, and cardiovascular processes (Sun et al, 2013;Zheng et al, 2015;Rahman, et al, 2016a). Neurological conditions like dementia, schizophrenia, and Parkinson's disease are likely to cause a deficiency or insufficient DA levels (Ali et al, 2007;Caudle et al, 2008;Guo et al, 2013).…”