l-carnitine supplementation during in vitro culture regulates oxidative stress in embryos from bovine aged oocytes

Jiang, Wen-Jie; Li, Yinghua; Zhao, Yuhan; Gao, Qingshan; Jin, Qingguo; Yan, Chunri; Xu, Yong‐Nan

doi:10.1016/j.theriogenology.2019.11.036

Cited by 22 publications

(13 citation statements)

References 39 publications

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“…However, the levels of BCL2L1 gene expression observed in our study were inconsistent with apoptosis levels assessed by TUNEL in fresh or vitri ed D7 blastocysts, suggesting that apoptosis detected by TUNEL is independent of the expression of BCL2L1 or BAX genes, as observed previously [48]. Similarly, mRNA levels of SOD1 were upregulated after SE treatment, indicating that a shorter exposure time may reduce oxidative stress by improving the activity of antioxidant enzymes and improving the quality of vitri ed/warmed embryos [49]. In addition, a trend although not signi cant (P = 0.07) was observed towards greater CX43 and AQP3 gene expression in blastocysts subjected to SE compared to LE.…”

Section: Discussioncontrasting

confidence: 75%

A shorter equilibration period in the VitTrans in-straw bovine embryo vitrification method improves post-warming outcomes

Martínez-Rodero¹,

García-Martínez²,

Ea³

et al. 2020

Preprint

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Background VitTrans is a device that enables the vitrification and warming/dilution of in vitro produced bovine embryos followed by their direct transfer to recipient females in field conditions. This study sought to improve the VitTrans method by comparing two equilibration times: short (SE: 3 min) and long (LE: 12 min). Outcome measures recorded in vitrified D7 and D8 expanded blastocysts were survival and hatching rates, differential cell counts, apoptosis rate and gene expression. Results While survival rates at 3 h and 24 h post-warming were reduced (P < 0.05) after vitrification, hatching rates of D7 embryos vitrified after SE were similar to those obtained in fresh non-vitrified blastocysts. Hatching rates of vitrified D8 blastocysts were lower (P < 0.05) than of fresh controls, regardless of treatment. Total cell counts, and inner cell mass and trophectoderm cell numbers were similar in hatched blastocysts derived from D7 blastocysts vitrified after SE and fresh blastocysts, while vitrified D8 blastocysts yielded lower values, regardless of treatment. The rate of apoptotic cells was significantly higher in both treatment groups when compared to fresh controls, although apoptosis rates were lower using the SE than LE protocol. No differences emerged in expression of the genes BAX, AQP3, CX43 and IFNτ between blastocysts vitrified after SE or LE, whereas a significantly higher abundance of BCL2L1 and SOD1 transcripts was observed in blastocysts vitrified after SE compared to LE. Conclusions The VitTrans device combined with a shorter exposure to the equilibration medium improves vitrification/warming outcomes facilitating the direct transfer of vitrified embryos under field conditions.

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Section: Discussioncontrasting

confidence: 75%

A shorter equilibration period in the VitTrans in-straw bovine embryo vitrification method improves post-warming outcomes

Martínez-Rodero¹,

García-Martínez²,

Ea³

et al. 2020

Preprint

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“…Experimental in vitro studies considered the effectiveness of antioxidants when given in combination. Truong and colleagues (2016, 2017) looked at the effects of the addition of a combination of triple antioxidants (10 µM acetyl- l -Carnitine, 10 µM N -acetyl-Cysteine- 5µM α-lipoic-acid) in the culture medium of mouse pronucleate oocytes and preimplantation embryos [ 78 , 79 ]. L-carnitine reduces ROS levels with its antioxidant actions and acts through the regulation and transport of long chain fatty acids into mitochondria for β-oxidation and ATP production.…”

Section: Agents Consideredmentioning

confidence: 99%

“…There was a beneficial effect of these combined antioxidants on embryo development [ 78 ], and a positive correlation between the presence of these antioxidants and increased blastocyst cells number [ 79 ]. The evidences about the efficacy of these compounds, administered alone in culture medium, in increasing the oocytes fertilization rate [ 93 , 94 ] and in improving embryo development [ 95 , 96 , 97 , 98 , 99 ] has also been highlighted in experimental studies [ 93 , 94 , 95 , 96 , 97 , 98 , 99 ].…”

Section: Agents Consideredmentioning

confidence: 99%

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Budani

Tiboni

2020

Antioxidants

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For most infertile couples, in vitro fertilization (IVF) represents the only chance to conceive. Given the limited success of IVF procedures, novel approaches are continuously tested with the aim of improving IVF outcomes. Growing attention is devoted today to the potential benefit of natural antioxidants in the optimization of infertility treatments. This review summarizes current data in this context, focusing on both experimental studies on oocytes/embryos and clinical trials on antioxidants supplementation. Based on information gained from experimental studies, antioxidant supplementation may have beneficial effects on IVF outcomes in terms of quality and cryotolerance of in vitro produced embryos, together with positive effects on in vitro maturation oocytes and on early embryonic development. Unfortunately, from the clinical side, there is a paucity of evidence favoring the protective qualities of antioxidants. Among the antioxidants considered, coenzyme Q10 may be regarded as one of the most promising for its positive role in rescuing the oxidative stress-induced damages, but further data are needed. It is concluded that further trials are necessary to characterize the potential clinical value of antioxidants in IVF treatments.

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“…L-Carnitine is also known as a potent antioxidant. Jiang et al (2020) showed that L-carnitine supplementation during the whole IVC can improve embryo development by reducing oxidative stress. In the present study L-carnitine was present in the IVC medium for 24 h, this time was probably not sufficient to protect against ROS, therefore no improvement was observed.…”

Section: Groupmentioning

confidence: 99%

Production ofin vitrobovine embryos supplemented withl-carnitine in different oxygen tensions and the relation to nitric oxide

Pereira

Cardoso

Silva

et al. 2020

Zygote

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Summary The aim of this study was to evaluate the production of bovine embryos in vitro when supplemented with l-carnitine for 24 h beginning on day 5 (d 5) under two different oxygen tensions (20% or 5%) and the relationship of nitric oxide (NO) in in vitro culture (IVC) medium to embryo development. Cumulus–oocyte complexes (COC; n = 837) were matured in vitro for 24 h and fertilization was performed for 18 h. Zygotes were cultured in vitro for 9 days after in vitro fertilization in synthetic oviductal fluid (SOF) medium with 5% fetal calf serum. At d 5 the plates were assigned to one of four treatment groups: high (20%) or low (5%) O2 tension either with or without the addition of 3.03 mM l-carnitine (High-Cont, High-Lcar, Low-Cont, Low-Lcar). The concentration of NO in the culture medium was evaluated on d 5, d 6 and d 9. On d 7, parts of the embryos were submitted for evaluation of intracellular lipid droplets. The cleavage rate was similar (P > 0.05) between high and low O2 tension and the blastocyst rate was similar in all conditions evaluated. The hatching rate was higher (P < 0.05) for Low-Cont. The NO concentration was higher at d 9 under low O2 tension (P < 0.1). The addition of 3.03 mM l-carnitine between d 5 and d 6 of IVC was not efficient in reducing cytoplasmic lipid content of bovine embryos. Additionally, IVC at a low oxygen tension without l-carnitine promoted better conditions for embryo development. A higher concentration of NO in medium was observed under low O2 tension.

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l-carnitine supplementation during in vitro culture regulates oxidative stress in embryos from bovine aged oocytes

Cited by 22 publications

References 39 publications

A shorter equilibration period in the VitTrans in-straw bovine embryo vitrification method improves post-warming outcomes

A shorter equilibration period in the VitTrans in-straw bovine embryo vitrification method improves post-warming outcomes

Effects of Supplementation with Natural Antioxidants on Oocytes and Preimplantation Embryos

Production ofin vitrobovine embryos supplemented withl-carnitine in different oxygen tensions and the relation to nitric oxide

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