L'alcool deshydrogénase de tomate (Lycopersicon esculentum): Étude cinétique et mécanisme d'action

Nicolas, Marie-Georgette; Crouzet, Jean; Aymard, Christian

doi:10.1016/0031-9422(80)83187-6

Cited by 4 publications

(1 citation statement)

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“…Alcohol dehydrogenase (ADH) is ubiquitously distributed in plant species and tissues and has been studied from a number of viewpoints, including genetics (Hanson and Brown 1984; Freeling and Bennett 1985), enzyme structure and catalysis (Nicolas et al 1980; Freeling and Bennett 1985), gene structure and promoter response elements (Dennis et al 1984; Dolferus et al 1994), tissue‐specific expression (Tanksley 1979; Newman and Van Toai 1992) and induction by a range of environmental stresses (Sachs and Freeling 1978; Matton et al 1990). Although Adh genes are, therefore, regarded as model plant genes, much less is known about the role of ADH in the physiology of the plant.…”

Section: Introductionmentioning

confidence: 99%

Regulation of alcohol dehydrogenase gene expression in barley aleurone by gibberellin and abscisic acid

Macnicol

Jacobsen

2001

Physiologia Plantarum

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The expression of the Adh1 gene (alcohol dehydrogenase, EC 1.1.1.1) was studied in the aleurone layer of barley (Hordeum vulgare cv. Himalaya). Expression increased markedly during grain development at the levels of activity, enzyme protein and mRNA. mRNA content, but not enzyme activity, could be increased further by exogenous abscisic acid (ABA) when isolated, de-embryonated developing grains were pre-treated with gibberellic acid (GA3) or fluridone. In isolated mature aleurone layers incubated with exogenous hormones, ADH mRNA was strongly up-regulated by ABA and down-regulated by GA3 within 6 h. With ABA, this increase in mRNA was followed by an increase in ADH protein and activity, peaking at 18 h. With GA3, the decrease in mRNA was accompanied by simultaneous decreases in protein and activity. In general, GA3 counteracted the effect of ABA and vice versa. In the aleurone of germinating grain, ADH activity decayed in a distal direction from the embryo, consistent with down-regulation by gibberellin(s) diffusing from it. It was concluded that ADH gene expression in the aleurone of the intact grain is regulated by an ABA/gibberellin interaction.

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