Regulation of alcohol dehydrogenase gene expression in barley aleurone by gibberellin and abscisic acid

Macnicol, Peter K.; Jacobsen, John V.

doi:10.1034/j.1399-3054.2001.1110414.x

Cited by 25 publications

(24 citation statements)

References 33 publications

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“…The expression patterns of several downregulated genes in antisense-RPK1 transgenic plants were confirmed by RNA gel blot analysis ( Figure 5A). Expression levels of other well-known genes regulated by ABA, such as ADH1 (MacNicol and Jacobsen, 2001), RD22, MYC2, AREB1, and several ERD genes (Shinozaki and YamaguchiShinozaki, 2000), also decreased in antisense-RPK1 plants. RNA gel blot analysis revealed that the expression levels of most ABAinducible genes were decreased in the rpk1-1 mutant ( Figure 5A).…”

Section: Rpk1 Regulates Aba-inducible Gene Expressionmentioning

confidence: 99%

Leucine-Rich Repeat Receptor-Like Kinase1 Is a Key Membrane-Bound Regulator of Abscisic Acid Early Signaling in Arabidopsis

et al. 2005

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Abscisic acid (ABA) is important in seed maturation, seed dormancy, stomatal closure, and stress response. Many genes that function in ABA signal transduction pathways have been identified. However, most important signaling molecules involved in the perception of the ABA signal or with ABA receptors have not been identified yet. Receptor-like kinase1 (RPK1), a Leu-rich repeat (LRR) receptor kinase in the plasma membrane, is upregulated by ABA in Arabidopsis thaliana. Here, we show the phenotypes of T-DNA insertion mutants and RPK1-antisense plants. Repression of RPK1 expression in Arabidopsis decreased sensitivity to ABA during germination, growth, and stomatal closure; microarray and RNA gel analysis showed that many ABA-inducible genes are downregulated in these plants. Furthermore, overexpression of the RPK1 LRR domain alone or fused with the Brassinosteroid-insensitive1 kinase domain in plants resulted in phenotypes indicating ABA sensitivity. RPK1 is involved in the main ABA signaling pathway and in early ABA perception in Arabidopsis.

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Section: Rpk1 Regulates Aba-inducible Gene Expressionmentioning

confidence: 99%

Leucine-Rich Repeat Receptor-Like Kinase1 Is a Key Membrane-Bound Regulator of Abscisic Acid Early Signaling in Arabidopsis

et al. 2005

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“…Abscisic acid (ABA) is required for reserve accumulation, and its involvement in the control of starch accumulation appears via SNF1 kinase, which mediates the phosphorylation state of some metabolic enzymes, and a set of transcriptional factors (Gutierrez et al, 2007). ABA and GA were found to coordinately regulate the expression of alcohol dehydrogenase in barley (Hordeum vulgare) seeds (Macnicol and Jacobsen, 2001), and cross talk between ABA and GA signaling has been found in rice seeds (Xie et al, 2006). Two putative GA receptor proteins were identified in rice seeds: one upregulated and the other down-regulated (Supplemental Table S4).…”

Section: Switch Between Central Carbon Metabolism and Alcoholic Fermementioning

confidence: 99%

Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains

et al. 2008

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Accumulation of reserve materials in filling grains involves the coordination of different metabolic and cellular processes, and understanding the molecular mechanisms underlying the interconnections remains a major challenge for proteomics. Rice (Oryza sativa) is an excellent model for studying grain filling because of its importance as a staple food and the available genome sequence database. Our observations showed that embryo differentiation and endosperm cellularization in developing rice seeds were completed approximately 6 d after flowering (DAF); thereafter, the immature seeds mainly underwent cell enlargement and reached the size of mature seeds at 12 DAF. Grain filling began at 6 DAF and lasted until 20 DAF. Dynamic proteomic analyses revealed 396 protein spots differentially expressed throughout eight sequential developmental stages from 6 to 20 DAF and determined 345 identities. These proteins were involved in different cellular and metabolic processes with a prominently functional skew toward metabolism (45%) and protein synthesis/destination (20%). Expression analyses of protein groups associated with different functional categories/subcategories showed that substantially upregulated proteins were involved in starch synthesis and alcoholic fermentation, whereas the down-regulated proteins in the process were involved in central carbon metabolism and most of the other functional categories/subcategories such as cell growth/division, protein synthesis, proteolysis, and signal transduction. The coordinated changes were consistent with the transition from cell growth and differentiation to starch synthesis and clearly indicated that a switch from central carbon metabolism to alcoholic fermentation may be important for starch synthesis and accumulation in the developmental process.Seed development is trigged by a double fertilization process specific to plants; after double fertilization, the fertilized egg cell develops into the embryo, and the fertilized polar nuclei develop into the endosperm (Goldberg et al., 1994). In dicotyledons, the endosperm is absorbed by the embryo during development, and reserve materials are stored in embryonic cotyledons (Goldberg et al., 1994;Le et al., 2007). However, in monocots such as cereal crops, the endosperm represents the main part of the mature seed and is an important organ for reserve storage (James et al., 2003). The cereal seed (also called the caryopsis) consists of the embryo, endosperm, and pericarp; the outermost endosperm cell layer differentiates into aleurone. Although seeds from different species are diverse in form, they have one common characteristic: accumulation of reserves during development, except for differences in reserve composition, such as approximately 85% of seed dry weight being starch in cereal seeds, 50% to 70% being fatty acids in oilseeds, and 40% being proteins in soybean (Glycine max) seeds (Ruuska et al., 2002). The reserve materials are not only essential for postembryonic growth and development by nourishing germinated embryos...

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“…Hence, hypoxia or anoxia is a frequent metabolic status even during normal development, particularly in tissues with a high O 2 demand and/or restricted O 2 entry (Geigenberger, 2003). ADH has a well established function in protection against hypoxic stress after flooding (Kennedy et al , 1992; Bailey-Serres and Voesenek, 2008), during seed development (Hanson et al , 1984; Macnicol and Jacobsen, 2001), and in aerobic metabolism in pollen (Buchner et al , 1995). Besides this, little is known about the role of ADH in the physiology of the plant.…”

Section: Introductionmentioning

confidence: 99%

“…ADH function in barley has been intensively studied with respect to the development of barley grains. The aleurone in the mature barley grain contains only ADH1 homodimers (Hanson et al , 1984) and ADH1 gene expression in the aleurone is regulated by an abscisic acid–gibberellic acid interaction (Macnicol and Jacobsen, 2001). In the course of the establishment of malate/ethanol fermentation in the aleurone layer during seed development there is a sequential change in the relative amount of ADH isozymes, with minor contributions of ADH2 and ADH3 (Macnicol and Jacobsen, 1992).…”

Section: Introductionmentioning

confidence: 99%

Alcohol dehydrogenase 1 of barley modulates susceptibility to the parasitic fungus Blumeria graminis f.sp. hordei

Pathuri

Reitberger

Hückelhoven

et al. 2011

Journal of Experimental Botany

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Plant primary energy metabolism is profoundly reorganized under biotic stress conditions and there is increasing evidence for a role for the fermentative pathway in biotic interactions. However, the mechanisms regulating metabolic reprogramming are not well understood despite its critical function in the biotic stress response. Here the function of alcohol dehydrogenase (ADH) in the interaction of barley with the parasitic fungus Blumeria graminis f.sp. hordei (Bgh) is addressed. Challenge of susceptible barley leaves with Bgh resulted in transcriptional activation of HvADH1 and an induction of ADH enzyme activity starting 24 h after infection and reaching a clear-cut effect 4 d after infection. This increase in ADH enzyme activity was not observed in the resistant near-isogenic mlo5 line. Moreover, an induction of ADH enzyme activity by Bgh was enhanced in the presence of sucrose in hydroponically grown seedlings. Transient knock-down or overexpression of HvADH1 in barley epidermal cells mediated a decrease or increase in the penetration success of Bgh, respectively. Inhibition of ADH activity by pyrazole resulted in a delay in symptoms. The pyrazole effect could be overcome by adding glucose to the incubation medium, pinpointing a nutritional effect of ADH in the barley–Bgh interaction. Taken together, misexpression of pathogen-inducible HvADH1 or variation of ADH activity modulates the pathogen response of barley to the biotrophic fungal parasite Bgh. In this way, ADH knock-down/inhibition results in reduced fungal success. The possibility is discussed that ADH activity supports biotrophy by maintaining glycolytic metabolism in pathogen-stressed barley.

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Regulation of alcohol dehydrogenase gene expression in barley aleurone by gibberellin and abscisic acid

Cited by 25 publications

References 33 publications

Leucine-Rich Repeat Receptor-Like Kinase1 Is a Key Membrane-Bound Regulator of Abscisic Acid Early Signaling in Arabidopsis

Leucine-Rich Repeat Receptor-Like Kinase1 Is a Key Membrane-Bound Regulator of Abscisic Acid Early Signaling in Arabidopsis

Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains

Alcohol dehydrogenase 1 of barley modulates susceptibility to the parasitic fungus Blumeria graminis f.sp. hordei

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