Kvantitativna analiza magnocelularnog dijela nucleus ruber-a

Abstract

Kratak sadržajUvod. Nucleus ruber zauzima centralni dio retikularne formacije srednjeg mozga, od donjeg kraja gornjih kvržica srednjeg mozga do ventralnog talamusa. Vaskularizuju ga grane P 1 i P 2 segmenta arteriae cerebri posterior, arteriae communicans posterior i arteriae choroidea anterior. Ciljevi rada su kvantitativna analiza neurona i vaskularne mreže magnocelularnog dijela nucleus ruber-a, kao i ispitivanje uticaja starenja na parametre kvantitativne analize.Metode. Deset srednjih mozgova odraslih lica oba pola je nakon fiksacije u 10% rastvoru formalina ukalupljeno u parafinu. Zbog lokalizacije magnocelularnog dijela nucleus ruber-a, uzorci srednjih mozgova su presječeni u visini sredine gornjih kvržica srednjeg mozga i idući kaudalno pravljeni su semiserijski rezovi (1,5,10...50) debljine 4 µm, koji su bojeni Mallory metodom. Pomoću digitalne kamere slikano je intermitentno svako drugo vidno polje pod uvećanjem 400x, a dobijene fotografije su analizirane pomoću programa ImageJ, uz korišćenje testnog sistema A 100. Statistička analiza je obavljena uz upotrebu programa SPSS, uz nivo značajnosti razlike od 5%.Rezultati. Prosječne vrijednosti kvantitativnih parametara neurona su iznosile: volumenska gustina 0,018 mm 0 , površinska gustina 3,56 mm -1 i apsolutni broj po vidnom polju 35,39. Prosječna volumenska gustina vaskularne mreže magnocelularnog dijela nucleus ruber-a je 0,014 mm 0 , površinska gustina 4,19 mm -1 i dužinska gustina 90,82 mm -2 . Nije postojala korelacija između godina života i određivanih kvantitativnih parametara neurona i vaskularne mreže magnocelularnog dijela nucleus ruber-a.Zaključak. Ispitivani kvantitativni parametri neurona i vaskularne mreže magnocelularnog dijela nucleus ruber-a se smanjuju sa godinama života, ali smanjenje nije statistički značajno.

Stereological analysis of the vascular network in superior colliculi

“…15 The volume density of the blood vessels of the magnocellular part of the nucleus ruber is 0.014 mm 0 , the surface density is 4.19 mm -1 , while the length density is 90.82 mm -2 . 16 All examined parameters of the vascular network of the SC were lower than the vascular density of the principal oculomotor nucleus and higher than the magnocellular part of the nucleus ruber. Based on these values, it is noticed that the blood vessels of the principal oculomotor nucleus are larger and more convoluted in comparison with the blood vessels of the SC and the magnocellular part of the nucleus ruber, which predisposes the principal oculomotor nucleus to a more frequent vascular occlusion.…”

“…Based on these values, it is noticed that the blood vessels of the principal oculomotor nucleus are larger and more convoluted in comparison with the blood vessels of the SC and the magnocellular part of the nucleus ruber, which predisposes the principal oculomotor nucleus to a more frequent vascular occlusion. 17,18 With ageing, no changes in the studied stereological parameters of the blood vessels of the principal oculomotor nucleus and the magnocellular part of the nucleus ruber were observed, 19,16 while the blood vessels of Discussion the SC show a decrease in the length density with age, which means that their tortuosity decreases, which was not seen in other structures at the same transversal level.…”

Changes of Neurons and Blood Vessels of Human Substantia Nigra in Aging- Morphometric Study

“…40 Our previous studies of neurons in the magnocellular part of the red nucleus have shown the reduction of the quantitative parameters with age, but the decrease did not reach statistical significance. 42 We mention this because of the proximity of red nucleus and SN at the same midbrain level, and there is a joint functional activity in the extrapyramidal system. This is confirmed by Lambert and associates, who showed the reduction in the number of neurons, gliosis and increased levels of iron with aging in SN and red nucleus.…”