Kutzneria chonburiensis sp. nov., isolated from soil

Chanama, Manee; Thongkrachang, Nongnat; Suriyachadkun, Chanwit; Chanama, Suchart

doi:10.1099/ijsem.0.000552

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…On the basis of the large 3 J H,H coupling constants (Table ) and NOESY correlations within each monosaccharide, Acu-A and Acu-B were identified as β-quinovose residues, while Acu-C was identified as the amino sugar β-acosamine (Figure S4). Thus, this trisaccharide was found to be identical to aculexitriose, previously found in aculeximycin (Figure ). An additional 3-amino-2,3-6-trideoxy-3-methylhexose unit comprising C1′′–C7′′ was constructed based on 2D NMR data (Figure ) and determined to be α-vancosamine based on the 3 J H,H coupling constants and the NOE cross-peaks (Figure S8a).…”

Section: Resultssupporting

confidence: 77%

“…Another example of an underexplored genus within Actinobacteria is Kutzneria , which belongs to the Pseudonocardiaceae family . At present, only five species of this genus have been reported: Kutzneria viridogrisea , Kutzneria albida , Kutzneria kofuensis , Kutzneria buriramensis , and Kutzneria chonburiensis .…”

Section: Introductionmentioning

confidence: 99%

“…15 At present, only five species of this genus have been reported: Kutzneria viridogrisea, Kutzneria albida, Kutzneria kofuensis, 15 Kutzneria buriramensis, 16 and Kutzneria chonburiensis. 17 Kutzneria albida DSM 43870T produces the antimicrobial macrolide aculeximycin (ACM), which has activity against fungi, Gram-positive bacteria, and mosquito larvae. 18 Whole genome sequencing (WGS) revealed that its genome spans 9 874 926 base-pairs (bp), coding for 8822 genes.…”

Section: ■ Introductionmentioning

confidence: 99%

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Discovery and Characterization of Epemicins A and B, New 30-Membered Macrolides from Kutzneria sp. CA-103260

et al. 2021

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Actinobacteria have been a rich source of novel, structurally complex natural products for many decades. Although the largest genus is Streptomyces, from which the majority of antibiotics in current and past clinical use were originally isolated, other less common genera also have the potential to produce a wealth of novel secondary metabolites. One example is the Kutzneria genus, which currently contains only five reported species. One of these species is Kutzneria albida DSM 43870T, which has 46 predicted biosynthetic gene clusters and is known to produce the macrolide antibiotic aculeximycin. Here, we report the isolation and structural characterization of two novel 30-membered glycosylated macrolides, epemicins A and B, that are structurally related to aculeximycin, from a rare Kutzneria sp. The absolute configuration for all chiral centers in the two compounds is proposed based on extensive 1D and 2D NMR studies and bioinformatics analysis of the gene cluster. Through heterologous expression and genetic inactivation, we have confirmed the link between the biosynthetic gene cluster and the new molecules. These findings show the potential of rare Actinobacteria to produce new, structurally diverse metabolites. Furthermore, the gene inactivation represents the first published report to genetically manipulate a representative of the Kutzneria genus.

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Section: Resultssupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Discovery and Characterization of Epemicins A and B, New 30-Membered Macrolides from Kutzneria sp. CA-103260

et al. 2021

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“…Kutzneria chonburiensis strain SMC256 T was isolated and taxonomically identified as described previously 39 . Briefly, it was isolated from a soil sample collected in a mountain forest in Chonburi province, Thailand.…”

Section: Methodsmentioning

confidence: 99%

Comparative genome features and secondary metabolite biosynthetic potential of Kutzneria chonburiensis and other species of the genus Kutzneria

Chanama

Prombutara

Chanama

2023

Sci Rep

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Actinobacteria are well known as a rich source of diversity of bioactive secondary metabolites. Kutzneria, a rare actinobacteria belonging to the family Pseudonocardiaceae has abundance of secondary metabolite biosynthetic gene clusters (BGCs) and is one of important source of natural products and worthy of priority investigation. Currently, Kutzneria chonburiensis SMC256T has been the latest type-strain of the genus and its genome sequence has not been reported yet. Therefore, we present the first report of new complete genome sequence of SMC256T (genome size of 10.4 Mbp) with genome annotation and feature comparison between SMC256T and other publicly available Kutzneria species. The results from comparative and functional genomic analyses regarding the phylogenomic and the clusters of orthologous groups of proteins (COGs) analyses indicated that SMC256T is most closely related to Kutzneria sp. 744, Kutzneria kofuensis, Kutzneria sp. CA-103260 and Kutzneria buriramensis. Furthermore, a total of 322 BGCs were also detected and showed diversity among the Kutzneria genomes. Out of which, 38 clusters showing the best hit to the most known BGCs were predicted in the SMC256Tgenome. We observed that six clusters responsible for biosynthesis of antimicrobials/antitumor metabolites were strain-specific in Kutzneria chonburiensis. These putative metabolites include virginiamycin S1, lysolipin I, esmeraldin, rakicidin, aclacinomycin and streptoseomycin. Based on these findings, the genome of Kutzneria chonburiensis contains distinct and unidentified BGCs different from other members of the genus, and the use of integrative genomic-based approach would be a useful alternative effort to target, isolate and identify putative and undiscovered secondary metabolites suspected to have new and/or specific bioactivity in the Kutzneria.

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“…One siderophore, one melanin, one lanthipeptide, Table 1. Phenotypic characteristics of strain AN110305 T and related species of the family Pseudonocardiaceae Taxa: 1, strain AN110305 T ; 2, Goodfellowiella [21,22]; 3, Umezawaea [53,54]; 4, Kutzneria [55][56][57][58]; 5, Saccharothrix [59][60][61][62][63][64]. Data were from this study unless indicated.…”

Section: Whole Genome Sequence Analysismentioning

confidence: 99%

Solihabitans fulvus gen. nov., sp. nov., a member of the family Pseudonocardiaceae isolated from soil

Jin

Liu

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A polyphasic taxonomic study was carried out on an actinobacterial strain (AN110305T) isolated from soil sampled in the Republic of Korea. Cells of the strain were Gram-stain-positive, aerobic, non-motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed a clear affiliation of strain AN110305T with Actinomycetia , with highest pairwise sequence similarities to Goodfellowiella coeruleoviolacea DSM 43935T (97.6%), Umezawaea tangerina MK27-91F2T (97.0%), Kutzneria chonburiensis NBRC 110610T (96.9%), Kutzneria buriramensis A-T 1846T (96.8%), Umezawaea endophytica YIM 2047XT (96.8%), Kutzneria albida NRRL B-24060T (96.7%) and Saccharothrix coeruleofusca NRRL B-16115T (96.6%). Cells of strain AN110305T formed pale-yellow colonies on Reasoner's 2A agar. MK-9 (H4) (68%) and MK-10 (H4) (32%) were the predominant menaquinones. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethyl ethanolamine, hydroxy-phosphatidylethanolamine, an unidentified aminolipid and an unidentified aminophospholipid were major polar lipids. Iso-C16:0 (24.5%), anteiso-C15:0 (19.3%), anteiso-C17:0 (15.7%) and iso-C15:0 (15.2%) were the major fatty acids and meso-diaminopimelic acid was the pepdidoglycan. The cell-wall sugars were composed of galactose, glucose, mannose and ribose. The genomic DNA G+C content was 70.7 mol%. Based on genotypic and phenotypic data, strain AN110305T could be distinguished from all genera within the family Pseudonocardiaceae and represents a novel genus and species named Solihabitans fulvus gen. nov., sp nov. The type strain is AN110305T (=KCTC 39307T =DSM 103572T).

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Kutzneria chonburiensis sp. nov., isolated from soil

Cited by 9 publications

References 31 publications

Discovery and Characterization of Epemicins A and B, New 30-Membered Macrolides from Kutzneria sp. CA-103260

Discovery and Characterization of Epemicins A and B, New 30-Membered Macrolides from Kutzneria sp. CA-103260

Comparative genome features and secondary metabolite biosynthetic potential of Kutzneria chonburiensis and other species of the genus Kutzneria

Solihabitans fulvus gen. nov., sp. nov., a member of the family Pseudonocardiaceae isolated from soil

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