Kurokura Solution as Immobilizing Medium for Spermatozoa of Tench (Tinca tinca L.)

Rodina, Marek; Cosson, Jacky; Gela, David; Linhart, Otomar

doi:10.1023/b:aqui.0000017192.75993.e3

Cited by 82 publications

(88 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is much published data available concerning the artificial reproduction of common tench under controlled conditions, but it mainly describes the reproduction of cultured stocks (e.g. Kouril et al 1986;Linhart and Billard 1995;Gela et al 2003;Rodina et al 2004;. It could be caused by many difficulties with capturing the fish from natural reservoirs.…”

Section: Introductionmentioning

confidence: 99%

Artificial spawning of common tench Tinca tinca (Linnaeus, 1758), obtained from wild and domestic stocks

et al. 2010

View full text Add to dashboard Cite

The study encompasses three reproduction seasons. Tench spawners caught during the spawning season originated from carp ponds (domestic stock) and a lake (wild stock). Fish were reproduced under controlled conditions after hormonal stimulation with GnRHa-containing pellets combined with metoclopramide (Ovopel) or carp pituitary homogenate (CPH). As a result of hormonal stimulation, eggs were obtained from a larger number of females originating from the lake (71.7%) than those originating from the pond (58.3%), although no other statistical differences were found. A similar relationship was recorded for the spermatozoa motilities (range from 72 to 76%). The obtained results indicate that both investigated reservoirs are suitable for tench broodstock management due to the fact that synchronization of ovulation among different stocks is easy to achieve. For this purpose, among the tested spawning agents, Ovopel could be recommended as being slightly more effective.

show abstract

Section: Introductionmentioning

confidence: 99%

Artificial spawning of common tench Tinca tinca (Linnaeus, 1758), obtained from wild and domestic stocks

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The parameters like seminal plasma composition, spermatozoa concentration, spermatocrit, and sperm motility traits have been used for evaluating the quality of sperm . Assessment of sperm quality based on these parameters provides us with applied approaches to improve methods for artificial reproduction by developing immobilizing or activating media for fertilization (Rodina et al, 2004). Studying the effects of these factors on sperm quality can help establish good activation and/or immobilizing media for improving either artificial fertilization or cryopreservation protocols.…”

mentioning

confidence: 99%

Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration

Golpour¹,

Akhoundian²,

Khara³

et al. 2013

CZECH J ANIM SCI

View full text Add to dashboard Cite

ABSTRACT:In this study, changes of pH, ionic (Na + , K + , Ca 2+ , and Mg 2+ ), biochemical (total protein, glucose, and cholesterol) compositions of seminal plasma, sperm motility traits (percentage of motile spermatozoa and sperm movement duration), and sperm production characteristics (sperm volume, spermatocrit, and sperm density) were studied in Caspian roach, Rutilus rutilus caspicus, during spawning migration. Sperm of 10 males was collected three times during the spawning migration (in February, March, and April). The results showed that sperm motility parameters (percentage of motile spermatozoa and sperm movement duration) changed significantly (P < 0.05) during the reproductive season, but sperm density, spermatocrit, and sperm volume did not show significant differences during spawning migration. Analyses performed at each sampling time (February, March, and April) showed significant differences (P < 0.05) in calcium, magnesium, potassium, and cholesterol, whereas there were no significant changes in Na + , pH, total protein, glucose, and cholesterol.

show abstract

“…At 120 s post-activation, spermatozoa motility decreased from 36-43% (0 h storage) to 0-5% (48-hour storage of sperm), but sperm velocity remained unchanged (11-13 µm/s at 0 h and 10 µm/s after 48 h). Decrease in spermatozoa motility and velocity has been frequently documented in fish species including common carp, Cyprinus carpio (Saad et al, 1988), halibut, Hippoglossus stenolepis (Billard et al, 1993), turbot, Psetta maxima (Chereguini et al, 1997), tench, Tinca tinca (Rodina et al, 2004), and Eurasian perch (Hatef et al, 2011). These decreases have been attributed to damage of spermatozoa ultrastructure and decrease of ATP content Hatef et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…These decreases have been attributed to damage of spermatozoa ultrastructure and decrease of ATP content Hatef et al, 2011). Rodina et al (2004) observed lower fertilizing ability of stored sperm of tench and suggested use of an immobilizing medium with osmolality similar or slightly higher than that of seminal plasma for sperm storage. In pikeperch, Korbuly et al (2009) studied incubation of sperm using various immobilizing media and observed better spermatozoa motility after 3 h incubation compared to a control when the sperm was previously diluted in Ringer's solution or PBS.…”

Section: Discussionmentioning

confidence: 99%

Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media

Křišťan¹,

Hatef²,

Alavi³

et al. 2014

CZECH J ANIM SCI

View full text Add to dashboard Cite

Spermatozoa morphology, ultrastructure, and spermatozoa motility traits were studied in pikeperch (Sander lucioperca) after activation in various media (AM 1 -45mM NaCl, 5mM KCl, 20mM Tris, pH 8.5; AM 2 -100mM sucrose, 20mM Tris, pH 8.5; AM 3 -100mM sucrose, 1mM CaCl 2 , 20mM Tris, pH 8.5) during a 48-hour storage period. The spermatozoon was acrosomeless and differentiated into a spherical nucleus (head), midpiece, and flagellum. The nucleus length and width measured 1.83 ± 0.03 and 1.63 ± 0.02 mm, respectively. The midpiece was located laterally to the nucleus and possessed proximal and distal centrioles and 2-4 mitochondria. Flagellar length was 33.2 ± 0.90 µm, and a pair of lateral fin-like structures projections was observed. The axoneme consisted of nine peripheral doublet microtubules and a single central pair. After a 24 h storage in all activation media at all sampling times post-activation (15, 45, 90, and 120 s), spermatozoa motility was significantly decreased. Spermatozoa were motile after the 48-hour storage at all sampling times post-activation only in AM 3. After the 48-hour storage, no motile spermatozoa were observed in AM 2 and AM 1 at 90 and 120 s post-activation, respectively. Differences in spermatozoa velocity varied with activation medium during storage. After the 48-hour storage in AM 1 and AM 2, decrease of spermatozoa velocity at 15 s post-activation was observed, while in AM 3, velocity was decreased only after the 48-hour storage. Pikeperch spermatozoa morphology and ultrastructure was found similar to that of most freshwater teleosts, with differences in the arrangement of midpiece, number of mitochondria, and position of centrioles. Viable pikeperch sperm was observed after the 48-hour storage. Motility of spermatozoa was improved by addition of Ca 2+ to the activation medium, where higher spermatozoa velocity was observed.

show abstract

Kurokura Solution as Immobilizing Medium for Spermatozoa of Tench (Tinca tinca L.)

Cited by 82 publications

References 26 publications

Artificial spawning of common tench Tinca tinca (Linnaeus, 1758), obtained from wild and domestic stocks

Artificial spawning of common tench Tinca tinca (Linnaeus, 1758), obtained from wild and domestic stocks

Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration

Sperm morphology, ultrastructure, and motility in pikeperch Sander lucioperca (Percidae, Teleostei) associated with various activation media

Contact Info

Product

Resources

About