Kupffer cell activation in liver preservation: Cold storage vs machine perfusion

Comparison of Vasosol and University of Wisconsin Solutions on Early Kidney Function After 24 Hours of Cold Ischemia in a Canine Autotransplantation Model

“…The advantages of cooling the graft and using these solutions during the recovery and implant process have been well documented [5,6]. However, there has been considerable interest in developing newer formulations of organ preserving solutions that target specific pathways of ischemia/reperfusion (I/R) injury, even within a particular organ [7].…”

Inhibition of TXA2 synthesis with OKY‐046 improves liver preservation by prolonged hypothermic machine perfusion in rats

“…We and others have previously demonstrated that Kupffer cells are the major source of TXA 2 production under some diseased conditions such as cirrhosis 26,49 and endotoxemia 27 . In addition, a number of studies have shown that Kupffer cells are activated in response to cold ischemia–warm reperfusion challenge 50–52 . These activated Kupffer cells were shown to be able to release inflammatory cytokines and vasoactive mediators including TXA 2 , which contributed to preservation‐induced liver injury 52 .…”

Improved rat liver preservation by hypothermic continuous machine perfusion using polysol, a new, enriched preservation solution

“…This solution has been further modified for application of MP in the liver, by substituting mannitol with raffinose. The resulting solution has been extensively used in experimental liver preservation10, 11, 14 but is not commercially available. We have developed a new preservation solution for MP of liver and kidney, Polysol, containing the nutrients that are in our view necessary to support the suppressed metabolism at 4°C.…”