Kultur Jaringan Kantong Semar (Nepenthes mirabilis)

Abstract

<p>ABSTRACT</p><p><br />This research was about in vitro propagation of Pitcher Plant Nepenthes mirabilis. The aims of this research were to determine: 1) the influence of kind and concentration of in vitro medium and also plant growth regulator on germination of Nepenthes mirabilis, 2)the effect of BAP and NAA on shoot <br />multiplication of N. mirabilis. This research consisted of three experiments and all experiments used Completely Randomized Design. On the first experiment MS (Murashige and Skoog) and KC (Knudson C) were used, i.e. full (1), three-fourth (3/4), one-half (1/2) and one-fourth (1/4) Salt Concentration. On the second experiment 10 composition media were used, i.e. ½ MS, ½ MS+TDZ 0.01 ppm, ½ MS+IAA 0.1 ppm, ½ MS+GA3 10 ppm, ½ MS+150 ml coconut water, 1/4KC, 1/4 KC+TDZ 0.01 ppm, ¼KC+IAA 0.1 ppm, 1/4KC+GA3 10 ppm and 1/4KC+ 150 ml coconut water. On the third experiment BAP with 0, 0.5, 1, 2 ppm and NAA 0, 0.1, 0.2, 0.5 ppm were used as factor for shoot multiplication.The result showed that KC medium was the best medium for germination percentage of N. mirabilis (64%). MS or KC medium with ½ or ¼ salt concentration was the suitable for germinating time of N. mirabilis (average 39 days after showing). Adding of TDZ, IAA, and GA3 significantly <br />increased germination percentage (70 – 90%) and decreased germinating time (27 – 38 days after showing). Culture growth on medium with BAP 0 - 1 ppm was the best medium for shoot and leave induction. The best medium for root growth of N. mirabilis was MS or KC medium without NAA.</p><p><br />Key words: Nepenthes mirabilis, MS, KC, TDZ, IAA, GA3, BAP, NAA, in vitro propagation, medium</p>

Effect of BAP (6-Benzyl Amino Purine) Concentration on Growth Micro Cutting of Nepenthes ampullaria

“…Since BAP promote cell division, it promotes leaflet development as well. This claim was supported byDinarti et al (2010) that BAP on MS media provide a statistically significant different effect on the number of N. mirabilis leaflet, BAP concentration within 0 -1 ppm could increase the number of…”

“…Leaflet development process requires low BAP; BAP that exceeds the optimum level can decrease the number of a leaflet. Dinarti et al (2010) stated that leaf development on in vitro culture was affected by cytokinin. Low concentration of BAP on culture media promoted more leaflet development while a high concentration of BAP, adversely, slowing down the growth and interrupting explant physiology.…”

“…Sayekti (2007) states that the growth regulating agent in the form of BAP (6-benzylaminopurine) from the cytokinin group can stimulate the formation of more buds. Giving BAP 0-1 ppm BAP could increase the number of leaves and buds on micro-culture of N. mirabilis (Dinarti et al, 2010) So far, no scientific repots were available on the effect of BAP addition on the growth of N. ampullaria in vito micro-cutting culture. Here we made experiments about the effect of different concentration of BAP on N. ampullaria growth.…”

In Vitro Planlet Induction of Tropical Pitcher Plant (Nepenthes Ampullaria Jack) by Various Thiamin and Benzyl Amino Purine Concentrate

“…Usaha konservasi ex-situ perlu dilakukan dengan cara domestikasi melalui mekanisme budidaya dan pemuliaan (Mansur, 2007). Menurut Witarto (2006) dalam Dinarti et al, (2010) Nepenthes diberi sebutan kantong semar karena ujung daunnya membentuk menjadi kantong seperti perut semar yang buncit. Kantong-kantong ini sangat menarik, karena bentuk dan warnanya yang indah.…”

PENGARUH ZAT PENGATUR TUMBUH TERHADAP MULTIPLIKASI TUNAS DAN BAHAN PENYANGGA PADA PEMBENTUKAN PLANTLET KANTONG SEMAR ADRIANII (Nepenthes adrianii) DENGAN KULTUR IN VITRO

“…Penelitian kultur in vitro pada tanaman kantong semar masih jarang dilakukan. Beberapa penelitian terkait dengan kultur in vitro tanaman kantong semar antara lain pernah dilakukan oleh Alitalia, 2008;Iqwal, 2008;Darmayanti et al, 2010;dan Dinarti et al, 2010. Namun demikian upaya menghasilkan plantlet yaitu tanaman kecil dengan akar, batang, dan daun yang sempurna dengan menggunakan beberapa media dan komposisi zat pengatur tumbuh, tidak memberikan hasil yang maksimal.…”