Kudiezi injection mitigates myocardial injury induced by acute cerebral ischemia in rats

Liu, Xuemei; Tao, Ye; Wang, Fengli; Yao, Ting; Fu, Chen; Hong, Zheng; Yan, Yan; Liang, Xiao; Jiang, Xiangning; Zhang, Yunling

doi:10.1186/s12906-016-1514-1

Cited by 12 publications

(9 citation statements)

References 27 publications

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“…Sun and Yang found that metformin improved the myocardial function of mice with heart failure following MI according to regulation of the expression of SIRT3 and PGC-1α in the mitochondrial inner membrane (58). However, mitochondrial dysfunction is not unique to MI; energy metabolism barriers can also occur in acute cerebral ischemia (59), renal ischemia (60), liver ischemia (61) and other ischemic diseases. This provides a novel direction in order to understand the process of MI, and further investigations are required.…”

Section: Discussionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantitation‑based proteomics reveals potential novel biomarkers for the early diagnosis of acute myocardial infarction within 3�h

Weng

Lou

et al. 2019

Int J Mol Med

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Acute myocardial infarction (AMI) is one of the most common and life-threatening cardiovascular diseases. However, the ability to diagnose AMI within 3 h is currently lacking. The present study aimed to identify the differentially expressed proteins of AMI within 3 h and to investigate novel biomarkers using isobaric tags for relative and absolute quantitation (ITRAQ) technology. A total of 30 beagle dogs were used for establishing the MI models successfully by injecting thrombin powder and a polyethylene microsphere suspension. Serum samples were collected prior to (0 h) and following MI (1, 2 and 3 h). ITRAQ-coupled liquid chromatography-mass spectrometry (LC-MS) technology was used to identify the differentially expressed proteins. The bioinformatics analysis selected several key proteins in the initiation of MI. Further analysis was performed using STRING software. Finally, western blot analysis was used to evaluate the results obtained from ITRAQ. In total, 28 proteins were upregulated and 23 were downregulated in the 1 h/0 h group, 28 proteins were upregulated and 26 were downregulated in the 2 h/0 h group, and 24 proteins were upregulated and 19 were downregulated in the 3 h/0 h group. The Gene Ontology (GO) annotation and functional enrichment analysis identified 19 key proteins. Protein-protein interactions (PPIs) were investigated using the STRING database. GO enrichment analysis revealed that a number of key proteins, including ATP synthase F1 subunit β (ATP5B), cytochrome c oxidase subunit 2 and cytochrome c , were components of the electron transport chain and were involved in energy metabolism. The western blot analysis demonstrated that the expression of ATP5B decreased significantly at all three time points (P<0.01), which was consistent with the ITRAQ results, whereas the expression of fibrinogen γ chain increased at 2 and 3 h (P<0.01) and the expression of integrator complex subunit 4 increased at all three time points (P<0.01), which differed from the ITRAQ results. According to the proteomics of the beagle dog MI model, ATP5B may serve as the potential biomarkers of AMI. Mitochondrial dysfunction and disruption of the electron transport chain may be critical indicators of early MI within 3 h. These finding may provide a novel direction for the diagnosis of AMI.

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Section: Discussionmentioning

confidence: 99%

Isobaric tags for relative and absolute quantitation‑based proteomics reveals potential novel biomarkers for the early diagnosis of acute myocardial infarction within 3�h

Weng

Lou

et al. 2019

Int J Mol Med

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“…Only levels of various serum inflammatory factors were measured, and other pathological processes involved in ischemic damage (e.g., oxidative stress) were not assessed. A previous study in rats showed that Kudiezi injection could decrease oxidative stress after stroke, as well as secondary myocardial damage in rats [ 25 ]. This aspect could be worth exploring in humans.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the balance between oxidative stress and antioxidant capacity is involved in the extent of damage after ischemic stroke [ 24 ]. Kudiezi injection has been shown to shift the balance toward decreased oxidative stress in rats [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Kudiezi Injection on Serum Inflammatory Biomarkers in Patients with Acute Cerebral Infarction

et al. 2018

Self Cite

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Background Kudiezi injection is a traditional Chinese medicine for acute cerebral infarction, but the exact mechanisms are poorly understood. Objective To investigate the mechanisms of Kudiezi injection on the inflammatory response in the treatment of acute cerebral infarction. Methods This was a prospective study of patients with acute cerebral infarction within 48 h of onset and treated between July 2012 and July 2016 at three hospitals in China. The patients were randomized to routine treatments (control group) versus routine treatments and Kudiezi injection (Kudiezi group). The National Institutes of Health Stroke Score was assessed on days 1, 3, 5, 7, and 14. The patients were tested for serum levels of pro- and anti-inflammatory cytokines (S100 calcium-binding protein B, neuron-specific enolase, interleukin-6, interleukin-10, interleukin-18, and matrix metaloproteinase-9; by enzyme-linked immunosorbent assay) immediately after admission and on days 3, 5, and 14. Results Stroke scores were improved in both groups from days 1 to 14. On days 5 and 7, stroke scores in the Kudiezi group were lower than in the control group (P < 0.05). Compared with controls, the Kudiezi group had lower serum S100 calcium-binding protein B on day 14; higher interleukin-6 and interleukin-10 on day 3; lower interleukin-6 and interleukin-18 on day 5; and lower interleukin-18 and matrix metaloproteinase-9 on day 14. Conclusion Kudiezi injection could lead to early reduction of interleukin-6, interleukin-18, matrix metaloproteinase-9, neuron-specific enolase, and S100 calcium-binding protein B levels and increases of interleukin-10 levels in patients with acute ischemic stroke. This trial is registered with ClinicalTrials.gov NCT01636154.

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“…Some herbal medicine injections produced by extracting and purifying active ingredients from herbs or decoctions according to modern scienti c and technological theories have often been used to treat some emergency and severe diseases in clinics in China. Kudiezi (KDZ) injection, a typical representative of herbal medicine injections, is extracted from the herb Ixeris denticulate and extensively applied to treat ischemic stroke and myocardial infarction [12]. Studies have shown that KDZ injection mainly contains avonoids, adenosine, sesquiterpene lactones and triterpene saponins [13].…”

Section: Introductionmentioning

confidence: 99%

Dissection of effect mechanisms of Kudiezi injection against myocardial ischemia based on virtual screening and systems pharmacology approach

Xie

Liu

Shi

et al. 2020

Preprint

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BackgroundKudiezi (KDZ) injection, a Chinese medicine preparation, has been widely used for the treatment of myocardial injury in recent years. However, the knowledge of the molecular mechanisms is limited to support its clinical application. It is of great importance to explore the underlying mechanisms of this preparation. MethodsIn this research, the 10 main ingredients in this injection were identified firstly by HPLC-Q-TOF-MS.Afterwards, virtual screening and an integrated systems pharmacology approach combined with molecular docking (molecular docking software: Sybyl-X 1.3 and Autodock vina) were adopted to predict its therapeutic mechanisms.Results 7 proteins targets and 9 KEGG pathways, possessing highly relevance to the therapeutic effects of KDZ against MI, were predicted reasonably. The systems analysis suggested that KDZ injection could exert its therapeutic effects against myocardial ischemia possibly through multi-targets including EGFR, MAPK10, SRC, et al, and multi-pathways referring to MAPK, Focal adhesion, Complement and coagulation cascades, Fluid shear stress and atherosclerosis, etc. ConclusionThis research provided a scientific basis to clarify the comprehensive pharmacological mechanism of KDZ injection acting on MI, and could provide valuable clues on considering the active ingredient KDZ-6 as a multi-targets leading compound.

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Kudiezi injection mitigates myocardial injury induced by acute cerebral ischemia in rats

Cited by 12 publications

References 27 publications

Isobaric tags for relative and absolute quantitation‑based proteomics reveals potential novel biomarkers for the early diagnosis of acute myocardial infarction within 3�h

Isobaric tags for relative and absolute quantitation‑based proteomics reveals potential novel biomarkers for the early diagnosis of acute myocardial infarction within 3�h

Effects of Kudiezi Injection on Serum Inflammatory Biomarkers in Patients with Acute Cerebral Infarction

Dissection of effect mechanisms of Kudiezi injection against myocardial ischemia based on virtual screening and systems pharmacology approach

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