Kualitas Semen Segar Sapi Bali Umur Produktif dan Non-produktif serta Penentuan Konsentrasi Krioprotektan dalam Pengencer Tris Kuning Telur

Abstract

The aim of this study was to compare the quality of Bali bull fresh semen in productive group (below 10 years old) with over productive group and to evaluate the frozen semen quality of bali bull after cryopreservation in Tris egg yolk extender using Dimethyl formamide (DMF) and glycerol in three different concentrations (5%, 6%, and 7%). Semen was collected using artificial vagina. Semen then evaluated macro and microscopically. Macroscopic evaluation conducted visually and microscopic evaluation with the aid of Computer Assisted Sperm Analysis CASA. Immediatelly after evaluation, semen were divided into six aliquots and diluted with Tris egg yolk (TEY) glycerol 5% (TEYG5), TEY-glycerol 6% (TEYG6), TEY glycerol 7% (TEYG7), TEY-DMF 5% (TKTD5), TEY-DMF 6% (TEYD6) and TEY-DMF 7% (TKTD7). Results of experiment showed that there was no difference (P>0.05) among the all parameters, except for semen volume. Semen volume of productive group was higher than over productive age group. No difference was found in post thawing motility of the spermatozoa in TEY extender with 5% glycerol, and TEY with 5%, 6% and 7% DMF. The higher recovery rate of bali bull spermatozoa demonstrated by spermatozoa in TEY extender with glycerol 5% and DMF 6% (63.33±2.40 dan 68.67±2.33). It was concluded that bali bull semen quality did not differ between productive and over productive ages and post thawing semen quality of bali bull in TEY with 5-7% DMF or 5% glycerol was not also different.

Correlation Between Testosterone Concentrations with Scrotal Circumference, and Semen Characteristics in Aceh Bulls

“…The differences in values reported might be related to species, age, body weight, ransom and environment of cattle used. These are in accordance to previous reports showing semen characteristics were influenced by age, fed quality, body weight, collection frequency and environment [27,28,29,32].…”

PENGARUH SUBTITUSI SARI BUAH SEMANGKA (Citrullus lanatus) DALAM PENGENCER SITRAT- KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI (Effect of watermelon juice supplementation in citrate - egg yolk extender on spermatozoa quality of bali bulls)

“…Hasil pengamatan semen segar setelah penampungan selama penelitian dapat dilihat pada Tabel 2. Dari hasil penampungan semen segar sapi bali (Tabel 2) diperoleh bahwa rataan volume semen 4,62±0,67 ml, hasil ini hampir sama dengan laporan Prastowo et Hasil pemeriksaan konsistensi semen segar adalah sedang, hal ini sama dengan penelitian Nabilla et al (2018) pada ternak sapi bali dengan konsistensi semen adalah sedang. Menurut Suyadi et al (2012) menyatakan bahwa warna, konsistensi dan konsentrasi spermatozoa mempunyai hubungan erat satu sama lain, artinya jika semen semakin encer maka konsentrasi spermatozoa semakin rendah dan warnanya semakin pucat.…”

Hubungan antara Konsentrasi Testosteron, Lingkar Skrotum, Libido dan Kuantitas Sperma Pejantan Sapi Pasundan

“…Volume semen dalam penelitian ini lebih tinggi (6,67±0,89 mL) dibandingkan dengan volume semen sapi pasundan hasil penelitian Darussalam et al (2020) sebesar 4,66 mL. Volume semen sapi pasundan ini hampir sama dengan volume semen sapi asli Indonesia yaitu sapi bali antara 6,32±0,07 mL (Indriastuti et al, 2020) dan 6,44±0,23 mL (Nabilla et al, 2018). Volume semen sapi pasundan sedikit lebih tinggi dari volume semen sapi madura yaitu sebesar 5,54±1,64 mL pada musim hujan dan 5,62±1,47 mL pada musim kemarau (Komariah et al, 2020) ataupun sapi aceh 3,82±0,47 mL (Zulyazaini et al, 2016).…”