KT2 and RT2 modified antimicrobial peptides derived from Crocodylus siamensis Leucrocin I show activity against human colon cancer HCT-116 cells

Anti-metastatic Effects of Cationic KT2 Peptide (a Lysine/Tryptophan-rich Peptide) on Human Melanoma A375.S2 Cells

“…A previous study showed that KT2 impaired the migration of HCT-116 colon cancer cells (25), but there is no report about the anti-metastasis effect of KT2 on A375.S2 human skin melanoma cells. Amphipathic cationic KT2 may be another possibility for the treatment of metastatic melanoma.…”

“…KT2 at concentrations of 5-40 μM significantly decreased A375.S2 cell growth at 24 h ( Figure 1A), and KT2-treated cells slightly exhibited cell morphological changes ( Figure 1B). However, KT2 was less cytotoxic to non-cancerous Vero cells and human red blood cells (22,25). Three concentrations of KT2 (2.5, 5, and 10 μM) were selected for further experiments because they led to a cell viability range of approximately 80-100%.…”

“…KT2 was found to inhibit the viability of cervical cancer HeLa cells (23) and induce apoptosis in colon cancer HCT-116 cells both in vitro and in vivo (24)(25)(26). Moreover, KT2 restrained the migration of HCT-116 cells (25). We hypothesized that cationic KT2 may have antimigration and anti-invasion activities against other types of cancer.…”

“…To enhance the antibacterial effect, the hydrophobic amino acid tryptophan (W) was added to expand the hydrophobic region, and lysine (K) was used to increase the hydrophilic part and charge (22). KT2 was found to inhibit the viability of cervical cancer HeLa cells (23) and induce apoptosis in colon cancer HCT-116 cells both in vitro and in vivo (24)(25)(26). Moreover, KT2 restrained the migration of HCT-116 cells (25).…”

The antitumor activity of 4,4′-bipyridinium amphiphiles

“…15 However, a variety of CAPs having cancerselective toxicity are peptide-based drugs that are difficult to be developed because of poor pharmacokinetics, potential systemic toxicity and high cost of manufacturing. [16][17][18] Nevertheless, a small molecular cationic antibacterial peptide possessing a simple structure and synthesis approach has been demonstrated to produce a strong antibacterial activity and a remarkable membrane cleavage effect. 19 Based on this idea, it was expected to develop a simple and an effective method to exploit cell membrane rupture cationic amphiphilic compounds and lay a foundation for the development of high-efficiency and low-toxic anti-cancer drugs.…”