Anti-metastatic Effects of Cationic KT2 Peptide (a Lysine/Tryptophan-rich Peptide) on Human Melanoma A375.S2 Cells

Maraming, Pornsuda; Klaynongsruang, Sompong; Boonsiri, Patcharee; Peng, Shu‐Fen; Daduang, Sakda; Rungsa, Prapenpuksiri; Tavichakorntrakool, Ratree; Chung, Jing‐Gung; Daduang, Jureerut

doi:10.21873/invivo.12250

Cited by 7 publications

(7 citation statements)

References 41 publications

(47 reference statements)

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“…We showed previously that the antimicrobial peptide P6 has a low toxicity against normal skin cells up to 200 µM [28], a concentration higher than the one we found to show effect against HCT-116 spheroids. Similar to our results, there were numerous tryptophan rich AMPs applied efficiently against cancer cells grown in monolayer [34][35][36][37]. However, we reported previously the effect of Gramicidn A against HT-29 spheroids showing that the peptide alone is efficient, destabilizing the spheroids, while in combination with a tumoral drug the effect is amplified [29].…”

Section: Pic Self-assemblies Increased Antitumor Potential Of P6 Against Hct-116 Spheroidssupporting

confidence: 90%

Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Răileanu

Lonetti

Serpentini

et al. 2022

Journal of Molecular Structure

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Antimicrobial peptides, a large class of molecules synthetized by various organisms as an innate defense against pathogens are more and more used for their anticancer properties as well. In order to overcome some of their limitations and to enhance their therapeutic efficiency, the use of delivery systems was taken into consideration. In this study we describe an original delivery system for antimicrobial peptides based on its physico-chemical properties, namely the selfassembled polyion complexes (PIC) based on electrostatic interactions of cationic antimicrobial peptide P6 with negatively charged double hydrophilic block copolymers, the poly(ethylene oxide)-poly(acrylic acid) (PEO-PAA) in our study. The drug delivery system was tested on 3D human tumor HCT-116 spheroids. The spheroid evolution and cell viability were monitored at 24 and 48 h after the treatment was applied. Our study demonstrates for the first time the feasibility of forming a polyion complex PIC with an antimicrobial peptide and that this self-assembled organisation provides added value in terms of anti-tumour therapeutic efficacy compared to the free form of the antimicrobial peptide.

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Section: Pic Self-assemblies Increased Antitumor Potential Of P6 Against Hct-116 Spheroidssupporting

confidence: 90%

Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Răileanu

Lonetti

Serpentini

et al. 2022

Journal of Molecular Structure

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“…Relatively, few studies have investigated the role of GRB2 in melanoma. One study showed that the cationic KT2 peptide downregulates GRB2 expression to inhibit the migration and invasion of human melanoma cells 25 . Another study indicated that phycocyanin hinders the proliferation of melanoma cells by downregulating GRB2/ERK signaling transduction 26 .…”

Section: Discussionmentioning

confidence: 99%

Hey1 promotes migration and invasion of melanoma cells via GRB2/PI3K/AKT signaling cascade

Lei

Chen

et al. 2021

J. Cancer

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Increasing evidence indicates that Notch signaling regulates multiple intracellular biological processes in malignant melanoma. Whereas how Notch signaling is transduced to influence melanoma cell behaviors remains largely elusive. Here we show that the Notch signaling downstream target Hey1 promotes migration and invasion of melanoma cells via the GRB2/PI3K/AKT pathway. First, bioinformatics tools, immunohistochemistry, and Western blotting analysis showed that the expression of Hey1 is increased in melanoma. Then, both in vivo and in vitro experiments showed that Hey1 promotes the malignant behaviour of the melanoma cells. High-throughput RNA-sequencing analysis revealed that inhibition of Hey1 results in decreased GRB2 expression in melanoma cells. Last, functional experiments confirmed that Hey1 positively regulates GRB2/PI3K/AKT pathway to influence migration and invasion of melanoma cells. In summary, our results suggest that Hey1 promotes the invasion and metastasis of melanoma cells by regulating GRB2/PI3K/AKT pathway. Our study provides potential therapeutics in tumor biology.

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“…Cell cytotoxicity (cell viability) was determined by MTT assay. GBM 8401/ luc2 cells (1 × 10 4 cells/well) were placed in 96-well plates for 24 h and then were treated with 0, 10, 15, 20, 25, 30, 35, 40, 45, and 50 μM of BDMC for 48 h. In brief, a 10 μL solution (5 mg/mL MTT) was added to each well for 4 h at 37 °C; discarded supernatant, followed a 100 μL DMSO, was added to well for dissolving the purple formazan crystalas described previously [ 48 ]. All treatment concentrations were performed in three independent tests.…”

Section: Methodsmentioning

confidence: 99%

“…GBM 8401/ luc2 cells (1 × 10 4 cells/well) were placed in 96-well plates for 24 h and then were pretreated with Z-DEVD-FMK (caspase-3 inhibitor) (25 μM) or inhibitor of mitochondria membrane potential (cyclosporin A) (1 μM) and were treated with 0 and 25 μM of BDMC for 48 h. Cells proliferation (viability) was measured by MTT assay as described previously [ 48 ]. All experiments were done in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…All membranes were incubated with primary antibodies (ant-pro-caspase-3, -active-caspase-3, -Bcl-2, -Bax, -cytochrome c, and -AIF) overnight, washed, and then incubated with peroxidase-labelled secondary antibody. The membranes were washed and incubated in ECL western blot analysis substrate (Bio-Rad, Hercules, CA, USA), and the protein bands were visualized and photographed as described previously [ 48 ]. All results were obtained from three independent experiments.…”

Section: Methodsmentioning

confidence: 99%

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Bisdemethoxycurcumin Induces Cell Apoptosis and Inhibits Human Brain Glioblastoma GBM 8401/Luc2 Cell Xenograft Tumor in Subcutaneous Nude Mice In Vivo

Hsia

Peng

Chueh

et al. 2022

IJMS

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Bisdemethoxycurcumin (BDMC) has biological activities, including anticancer effects in vitro; however, its anticancer effects in human glioblastoma (GBM) cells have not been examined yet. This study aimed to evaluate the tumor inhibitory effect and molecular mechanism of BDMC on human GBM 8401/luc2 cells in vitro and in vivo. In vitro studies have shown that BDMC significantly reduced cell viability and induced cell apoptosis in GBM 8401/luc2 cells. Furthermore, BDMC induced apoptosis via inhibited Bcl-2 (anti-apoptotic protein) and increased Bax (pro-apoptotic proteins) and cytochrome c release in GBM 8401/luc2 cells in vitro. Then, twelve BALB/c-nude mice were xenografted with human glioblastoma GBM 8401/luc2 cancer cells subcutaneously, and the xenograft nude mice were treated without and with BDMC (30 and 60 mg/kg of BDMC treatment) every 3 days. GBM 8401/luc2 cell xenografts experiment showed that the growth of the tumors was significantly suppressed by BDMC administration at both doses based on the reduction of tumor size and weights. BDMC did not change the body weight and the H&E histopathology analysis of liver samples, indicating that BDMC did not induce systemic toxicity. Meanwhile, treatment with BDMC up-regulated the expressions of BAX and cleaved caspase-3, while it down-regulated the protein expressions of Bcl-2 and XIAP in the tumor tissues compared with the control group. This study has demonstrated that BDMC presents potent anticancer activity on the human glioblastoma GBM 8401/luc2 cell xenograft model by inducing apoptosis and inhibiting tumor cell proliferation and shows the potential for further development to the anti-GBM cancer drug.

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Anti-metastatic Effects of Cationic KT2 Peptide (a Lysine/Tryptophan-rich Peptide) on Human Melanoma A375.S2 Cells

Cited by 7 publications

References 41 publications

Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Encapsulation of a cationic antimicrobial peptide into self-assembled polyion complex nano-objects enhances its antitumor properties

Hey1 promotes migration and invasion of melanoma cells via GRB2/PI3K/AKT signaling cascade

Bisdemethoxycurcumin Induces Cell Apoptosis and Inhibits Human Brain Glioblastoma GBM 8401/Luc2 Cell Xenograft Tumor in Subcutaneous Nude Mice In Vivo

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