Kriopreservasi Semen Domba Garut Menggunakan Tris Kuning Telur yang Disuplementasi Omega-3 Minyak Ikan Salmon (CRYOPRESERVATION GARUT SHEEP SEMEN USING TRIS EGG YOLK SUPPLEMENTED OMEGA-3 FISH OIL SALMON)

Nurcholis, Nurcholis; Arifiantini, Raden Iis; Yamin, Mohamad

doi:10.19087/jveteriner.2016.17.2.309

Cited by 5 publications

(6 citation statements)

References 8 publications

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“…Supplementation of RFO in TEY diluents is important to prevent sperm damage and maintain the balance of osmotic pressure during freezing. Nurcholis et al (2016) stated that the formation of ice crystals could damage the sperm membrane. Damage to the membrane in the midpiece can interfere with ATP formation by the mitochondria, thereby reducing the percentage of sperm motility.…”

Section: Discussionmentioning

confidence: 99%

“…The proper handling of frozen semen is important to avoid sperm damage. The damage that occurred during freezing can be influenced by several factors, including the diluent factor, the semen freezing process, and storage (Nurcholis et al, 2016). Diluents containing cryoprotectants such as tris-aminomethane play an essential role in maintaining the quality of semen in the freezing process (Suyadi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The oxidative stress caused by cold shock and lipid peroxidation in the cell membrane leads to the decreased quality of the sperm. The plasma membrane of sperm of ruminant consists of polyunsaturated fatty acids (PUFA) which are susceptible to lipid peroxidation (Nurcholis et al, 2016). Lipid peroxidation in sperm changes the structure of its cell membrane.…”

Section: Introductionmentioning

confidence: 99%

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Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Nurcholis¹,

Furqon

Arifiantini

et al. 2021

Trop. Anim. Sci. J.

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Antioxidants such as tocopherol, ß-carotene, and polyunsaturated fatty acids (PUFA) from red fruit oil of Papua may be used to protect frozen semen. The study is aimed to test the effect of red fruit oil supplementation on motility, viability, and recovery rate of frozen sperm of Ongole-grade bulls. Semen was collected twice a month from eight 4-5-year-old male Ongole grade using an artificial vagina, followed by macro- and microscopical evaluations. Collected semen was divided into four tubes and diluted with tris egg yolk diluents (TEY) as a control, TEY supplemented with 0.5% red fruit oil (RFO) (TEY RFO0.5), TEY supplemented with 1% RFO (TEY RFO1.0), and TEY supplemented with 1.5% RFO (TEY RFO1.5). The diluted semen was then packed into the straw and equilibrated for 2, 4, and 6 h prior to frozen on liquid nitrogen vapor for 10 minutes. The observed variables in this study were sperm motility, sperm viability, and morphology after equilibration, after thawing, and recovery rate. The experimental design is a completely randomized factorial design. The data were analyzed using ANOVA and were further tested using Duncan multiple range test. The results showed that the sperm motility of fresh semen was 81.10±1.42%. The percentage of sperm motility in TEY RFO1.5 treatment at 6 h equilibration was 60.00±1.06%, significantly higher compared to TEY RFO1.0 and TEY RFO0.5. The percentage of post-thawing sperm motility in TEY RFO1.5 treatment was 62.40±1.09%. The best post-thawing sperm viability in TEY RFO1.5 was 80.70±1.20%, significantly increase from the treatment of TEY RFO1.0 and TEY RFO0.5. The recovery rate (RR) for TEY RFO1.5 treatment had the best percentage at 76.94%. In conclusion, RFO supplementation in semen diluents for 2 h of equilibration resulted in the best motility and viability at 0 h of post thawing observation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Nurcholis¹,

Furqon

Arifiantini

et al. 2021

Trop. Anim. Sci. J.

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“…Upaya yang dapat dilakukan untuk memenuhi kebutuhan energi pakan adalah pemanfaatan minyak ikan salmon yang umum dipasarkan dalam bentuk kapsul dan memiliki kandungan energi tinggi (Khasanah et al, 2012;Handayani et al, 2015). Minyak ikan mengandung sekitar 25% asam lemak jenuh dan 75% asam lemak tak jenuh (Nurcholis et al, 2016). Minyak ikan merupakan sumber asam lemak omega 3 rantai panjang dan sangat rentan terhadap oksidasi.…”

Section: Pendahuluanunclassified

EFEK PEMBERIAN MINYAK IKAN SALMON (Oncorhynchus masou) TERHADAP PRODUKTIVITAS AYAM PETELUR ISA BROWN

Zulkarnain¹,

Badaruddin²,

Munadi³

2021

JPN

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AbstrakTujuan penelitian ini adalah untuk mengetahui pengaruh pemberian minyak ikan salmon terhadap konsumsi pakan, berat telur, produksi telur dan konversi pakan ayam petelur Isa brown. Penelitian ini dilaksanakan di Laboratorium Ilmu Teknologi dan Produksi Ternak Unit Ternak Unggas Fakultas peternakan Universitas Halu Oleo pada bulan November 2019 selama 1 bulan. Materi penelitian ini adalah 16 ekor ayam petelur Strain Isa brown yang berumur 8 bulan. Variabel yang diamati dalam penelitian ini adalah konsumsi pakan, berat telur, produksi telur dan konversi pakan. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap yang terdiri dari 4 perlakuan dan 4 ulangan dengan pemberian minyak ikan salmon yang terdiri dari: kontrol tanpa minyak ikan (P0), 2 kapsul/ekor/hari (P1), 4 kapsul/ekor/hari (P2) dan 6 kapsul/ekor/hari (P3). Hasil penelitian ini menunjukkan bahwa pemberian kapsul minyak ikan salmon dosis 2 sampai 6 kapsul/ekor/hari belum memberikan pengaruh nyata terhadap konsumsi pakan, berat telur, produksi telur dan konversi pakan ayam petelur Isa brown.Kata kunci: Ayam petelur, Produktivitas, minyak ikan.

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“…Santosa et al, (2013) mengungkapkan, pemberian ransum dengan penambahan kombinasi vitamin A dan E mampu menurunkan kadar kolesterol darah. Perubahan kolesterol dalam darah dipengaruhi oleh biosintesis kolesterol dan sintesis asam empedu serta variasi kolesterol dalam jaringan tubuh, dan perbaikan sel, Nurcholis et al, (2016)…”

Section: Tabel 1 Analisis Kandungan Kolesterol Padaunclassified

Efektifitas Jamu Herbal Terhadap Kadar Lemak Dan Kolesterol Daging Ayam Petelur Umur 26 Bulan

Nurcholis¹

2020

JPN

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Potensi ayam petelur di merauke cukup tinggi, dan pada ahirnya ayam-ayam ini akan dijadikan ayam afkir. Ayam afkir memiliki kekurangan selain karkas dagingnya cukup kenyal dan keras, ayam ini memiliki banyak lemak abdomen dan kandungan kolesterol. Jamu herbal memiliki kelebihan diantaranya kandungan antioksidan, minyak atsiri dan kurkumin dalam tumbuhan herbal mampu menurunkan kadar lemak dan kolesterol. Penelitian ini bertujuan untuk mengetahui efektifitas jamu herbal terhadap kandungan persentase kolesterol daging dan lemak abdomen. Penelitian dilaksanakan selama 1 bulan, menggunakan 36 ekor ayam afkir petelur umur 26 bulan. Bahan penelitian menggunakan jamu herbal yang terdiri dari beberapa jensi tumbuhan diantaranya : Buah merah 30%; Kunyit 20%; Jahe 5%; temu lawak 25%; Kencur 20%. Hasil penelitian menunjukkan bahwa jamu herbal yang diberikan tidak memberikan pengaruh (P> 0.05) terhadap kolesterol R0 yaitu 131 mg, R1 sebanyak 130, 67 mg, R2 sebanyak 128,33 mg, dan R3 sebanyak 126 mg dan lemak R0 (4.6 %), R1 (4.23 %), R2 (4. 43 %), R3 (4.5 %), namun nilai persentase menunjukkan adanya kecenderungan menurun. Hal ini diduga pemberian jamu herbal cukup sedikit sehingga belum maksimal dalam mempengaruhi kadar kolesterol dan lemak.

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Kriopreservasi Semen Domba Garut Menggunakan Tris Kuning Telur yang Disuplementasi Omega-3 Minyak Ikan Salmon (CRYOPRESERVATION GARUT SHEEP SEMEN USING TRIS EGG YOLK SUPPLEMENTED OMEGA-3 FISH OIL SALMON)

Cited by 5 publications

References 8 publications

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

Supplementation of Pandanus conoideus Oil in Cryopreservation Diluents for Maintaining the Semen Quality of Ongole Grade Bull

EFEK PEMBERIAN MINYAK IKAN SALMON (Oncorhynchus masou) TERHADAP PRODUKTIVITAS AYAM PETELUR ISA BROWN

Efektifitas Jamu Herbal Terhadap Kadar Lemak Dan Kolesterol Daging Ayam Petelur Umur 26 Bulan

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