Krill Oil Ameliorates Mitochondrial Dysfunctions in Rats Treated with High-Fat Diet

Ferramosca, Alessandra; Conte, Annalea; Zara, Vincenzo

doi:10.1155/2015/645984

Cited by 25 publications

(23 citation statements)

References 34 publications

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“…In this context, OO consumption is encouraged, as it has been associated to a lower prevalence of chronic diseases and hepatic disorders (Sofi & Casini, ; Velasco et al ., ). On the other hand, KO has become increasingly popular because of its capability of reducing hepatic lipogenesis, stimulating catabolism of fat excess introduced by a HF diet and ameliorating mitochondrial function (Ferramosca et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…We also found that fatty acid composition of the diet was able to influence the activity of some enzymes of the Krebs cycle, such as CS, aconitase and fumarase. A strong and significant negative correlation of CS with obesity was reported in liver (Ferramosca et al ., ) and in human omental adipose tissue (Christe et al ., ). At the same time, an increase in CS activity was recently observed after KO diet supplementation in rat liver (Ferramosca et al ., ), suggesting that a modulation of the activity of this protein, which is the rate‐limiting enzyme of the Krebs cycle, could regulate the flux of reducing equivalents to mitochondria.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the effect of KO was more pronounced than that of OO. The presence of a fatty acid modulation of respiratory complexes activity, which was previously observed in liver mitochondria (Ferramosca et al ., ), was confirmed by measuring the activity of single components of the sperm respiratory chain spectrophotometrically.…”

Section: Discussionmentioning

confidence: 99%

“…Mitochondrial respiratory complexes activities were also evaluated spectrophotometrically, essentially as described in (Ferramosca et al ., ).…”

Section: Methodsmentioning

confidence: 97%

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Dietary fatty acids influence sperm quality and function

et al. 2017

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Recently, obesity has been linked to male infertility. In animal models the administration of a high-fat diet caused a reduction in sperm quality, by impairing gamete energy metabolism. The aim of this study was to investigate a possible effect of dietary fatty acids supplementation in the modulation of sperm energy metabolism and, in turn, in the improvement of sperm quality in rats fed a high-fat diet. Sexually mature male Sprague-Dawley rats were divided into four groups and fed for 4 weeks a standard diet (control group), a high-fat diet (enriched in 35% of fat and 15% sucrose), a high-fat diet supplemented with 2.5% olive oil (a source of monounsaturated fatty acids) or a high-fat diet supplemented with 2.5% krill oil (a source of n-3 polyunsaturated fatty acids). Liver and adipose tissue weight, plasma glucose, insulin and lipid concentrations were determined. Activities of enzymes involved in sperm energetic metabolism were evaluated by spectrophotometric assays. Sperm mitochondrial respiratory efficiency was also assayed. The obtained results suggest that olive oil partially counteracts the negative effects of a high-fat diet on sperm quality, by increasing gamete motility, by reducing oxidative stress and slightly improving mitochondrial respiration efficiency. On the other hand, krill oil determines an increase in sperm concentration and motility, an increase in the activities of lactate dehydrogenase, Krebs cycle enzymes and respiratory chain complexes; a parallel increase in the cellular levels of ATP and a reduction in oxidative damage were also observed. These results suggest that dietary fatty acids are able to positively influence sperm quality and function.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Mitochondrial respiratory complexes activities were also evaluated spectrophotometrically, essentially as described in (Ferramosca et al ., ).…”

Section: Methodsmentioning

confidence: 97%

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Dietary fatty acids influence sperm quality and function

et al. 2017

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“…Previous studies showed that citrate synthase activity was suppressed in obese mice, resulting in excessive carbon flow into the citrate cycle prompting energy storage [44]. Gut microbiota appears to play a key role in the development and progression of obesity, together with changes in citrate synthase activity [45, 46]. In this study, analysis of the results of whole microbial genome sequencing suggested that enrichment in the microbial function that relates to citrate cycle was significantly different between the fat and lean lines (Fig.…”

Section: Discussionmentioning

confidence: 99%

Divergent selection-induced obesity alters the composition and functional pathways of chicken gut microbiota

et al. 2016

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BackgroundThe gastrointestinal tract is populated by a complex and vast microbial network, with a composition that reflects the relationships of the symbiosis, co-metabolism, and co-evolution of these microorganisms with their host. The mechanism that underlies such interactions between the genetics of the host and gut microbiota remains elusive.ResultsTo understand how genetic variation of the host shapes the gut microbiota and interacts with it to affect the metabolic phenotype of the host, we compared the abundance of microbial taxa and their functional performance between two lines of chickens (fat and lean) that had undergone long-term divergent selection for abdominal fat pad weight, which resulted in a 4.5-fold increase in the fat line compared to the lean line. Our analysis revealed that the proportions of Fusobacteria and Proteobacteria differed significantly between the two lines (8 vs. 18% and 33 vs. 24%, respectively) at the phylum level. Eight bacterial genera and 11 species were also substantially influenced by the host genotype. Differences between the two lines in the frequency of host alleles at loci that influence accumulation of abdominal fat were associated with differences in the abundance and composition of the gut microbiota. Moreover, microbial genome functional analysis showed that the gut microbiota was involved in pathways that are associated with fat metabolism such as lipid and glycan biosynthesis, as well as amino acid and energy metabolism. Interestingly, citrate cycle and peroxisome proliferator activated receptor (PPAR) signaling pathways that play important roles in lipid storage and metabolism were more prevalent in the fat line than in the lean line.ConclusionsOur study demonstrates that long-term divergent selection not only alters the composition of the gut microbiota, but also influences its functional performance by enriching its relative abundance in microbial taxa. These results support the hypothesis that the host and gut microbiota interact at the genetic level and that these interactions result in their co-evolution.Electronic supplementary materialThe online version of this article (doi:10.1186/s12711-016-0270-5) contains supplementary material, which is available to authorized users.

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Obesity and Male Infertility: Role of Fatty Acids in the Modulation of Sperm Energetic Metabolism

Ferramosca

Giacomo

Moscatelli

et al. 2018

Euro J Lipid Sci & Tech

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Obesity in men of reproductive age is increasing worldwide, impacting negatively on reproductive potential, sperm function, and assisted reproduction outcomes. Changes in modern eating behaviors are needed to invert the negative correlation between lifestyle and sperm quality. It is known from the literature that fatty acids are able to modulate lipid metabolism by complex mechanisms involving a sequence of interdependent and cross‐regulated molecular events. Although a great deal of data is currently available on the dietary modulation of lipid metabolism, little is known about the nutritional regulation of sperm energetic metabolism. In this review, major data from the literature about the role of some dietary fats as modulators of sperm function and quality are described. Moreover, biochemical mechanisms responsible for an impairment or improvement in sperm bioenergetics are critically analyzed. It is noteworthy that both quantitative and qualitative aspects of dietary fats influence sperm function. The administration of a high‐fat diet, low in polyunsaturated fatty acids, caused a significant increase in body weight hyperglycemia, hyperinsulinemia, and dyslipidemia, along with a significant reduction in sperm quality. In contrast, unsaturated fatty acids are able to positively influence sperm quality and function. The understanding of the biochemical mechanisms underlying the development of male infertility related to obesity led us to depict a possible framework for the molecular action of fatty acids on sperm quality during dysmetabolic conditions. Practical Applications: In this review major data from the literature about the spermatozoa targeting of dietary fatty acids are described and critically analyzed, because now dietary fatty acids are the current focus of research in the field of nutrition and reproduction in males. Such knowledge will eventually translate into the development of new attractive therapeutic approaches for male infertility related to alterations in lipid metabolism. Dietary fatty acids are involved in the nutritional modulation of sperm energetic metabolism. Diets rich in saturated fatty acids (SFA) and low in polyunsaturated fatty acids (PUFA) seem to correlate with defective sperm energy metabolism and reduced sperm quality. Administration of PUFA, especially n‐3 PUFA, determines an increase in sperm concentration, and motility, associated to an increase in the activities of mitochondrial enzymes involved in gamete energetic metabolism and to a reduction in oxidative damaeg

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Krill Oil Ameliorates Mitochondrial Dysfunctions in Rats Treated with High-Fat Diet

Cited by 25 publications

References 34 publications

Dietary fatty acids influence sperm quality and function

Dietary fatty acids influence sperm quality and function

Divergent selection-induced obesity alters the composition and functional pathways of chicken gut microbiota

Obesity and Male Infertility: Role of Fatty Acids in the Modulation of Sperm Energetic Metabolism

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