KPC-3-producing ST167 Escherichia coli from mussels bought at a retail market in Tunisia

Mani, Yosra; Mansour, Wejdène; Mammeri, Hedi; Denamur, Erick; Saras, Estelle; Boujaafar, N.; Bouallègue, Olfa; Madec, Jean‐Yves; Haenni, Marisa

doi:10.1093/jac/dkx124

Cited by 27 publications

(21 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ESBL-producing MDR and XDR E. coli isolates with sequence types ST167 and ST617 and others in the ST10 clonal complex have emerged over the past 5 years as common isolates from nosocomial sources as well as wild animal and domestic animal sources, including dairy and livestock sources, in Germany (45–47), Taiwan (48, 49), Tunisia (50, 51), and the Americas (52). Indeed, ST167 strains carrying carbapenemase activity have become the second most prevalent sequence type, behind only ExPEC ST131, among human clinical ESBL-producing E.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genome Analysis of an Extensively Drug-Resistant Isolate of Avian Sequence Type 167 Escherichia coli Strain Sanji with Novel In Silico Serotype O89b:H9

Zhang

Chi

et al. 2019

mSystems

View full text Add to dashboard Cite

Extensive drug resistance (XDR) is an escalating global problem. Escherichia coli strain Sanji was isolated from an outbreak of pheasant colibacillosis in Fujian province, China, in 2011. This strain has XDR properties, exhibiting sensitivity to carbapenems but no other classes of known antibiotics. Whole-genome sequencing revealed a total of 32 known antibiotic resistance genes, many associated with insertion sequence 26 (IS26) elements. These were found on the Sanji chromosome and 2 of its 6 plasmids, pSJ_255 and pSJ_82. The Sanji chromosome also harbors a type 2 secretion system (T2SS), a type 3 secretion system (T3SS), a type 6 secretion system (T6SS), and several putative prophages. Sanji and other ST167 strains have a previously uncharacterized O-antigen (O89b) that is most closely related to serotype O89 as determined on the basis of analysis of the wzm-wzt genes and in silico serotyping. This O89b-antigen gene cluster was also found in the genomes of a few other pathogenic sequence type 617 (ST617) and ST10 complex strains. A time-scaled phylogeny inferred from comparative single nucleotide variant analysis indicated that development of these O89b-containing lineages emerged about 30 years ago. Comparative sequence analysis revealed that the core genome of Sanji is nearly identical to that of several recently sequenced strains of pathogenic XDR E. coli belonging to the ST167 group. Comparison of the mobile elements among the different ST167 genomes revealed that each genome carries a distinct set of multidrug resistance genes on different types of plasmids, indicating that there are multiple paths toward the emergence of XDR in E. coli. IMPORTANCE E. coli strain Sanji is the first sequenced and analyzed genome of the recently emerged pathogenic XDR strains with sequence type ST167 and novel in silico serotype O89b:H9. Comparison of the genomes of Sanji with other ST167 strains revealed distinct sets of different plasmids, mobile IS elements, and antibiotic resistance genes in each genome, indicating that there exist multiple paths toward achieving XDR. The emergence of these pathogenic ST167 E. coli strains with diverse XDR capabilities highlights the difficulty of preventing or mitigating the development of XDR properties in bacteria and points to the importance of better understanding of the shared underlying virulence mechanisms and physiology of pathogenic bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative Genome Analysis of an Extensively Drug-Resistant Isolate of Avian Sequence Type 167 Escherichia coli Strain Sanji with Novel In Silico Serotype O89b:H9

Zhang

Chi

et al. 2019

mSystems

View full text Add to dashboard Cite

show abstract

“…The first one is the potential unsafety of food linked to contaminated water, preparation, or poor hygiene [ 35 , 36 , 37 , 38 ]. Vegetables and mussels contaminated with Gram-negative carriers of ESBL or KPC-3 carbapenemase in retail markets in North Africa, or imported seafood and raw dog food with the presence of mcr-1 positive E. coli isolates in Norway, have been recently reported, thus highlighting new potential pathways to transfer AMR genes to “low prevalence” countries [ 39 , 40 , 41 , 42 , 43 ].…”

Section: Amr In Animalsmentioning

confidence: 99%

Country Income Is Only One of the Tiles: The Global Journey of Antimicrobial Resistance among Humans, Animals, and Environment

et al. 2020

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is one of the most complex global health challenges today: decades of overuse and misuse in human medicine, animal health, agriculture, and dispersion into the environment have produced the dire consequence of infections to become progressively untreatable. Infection control and prevention (IPC) procedures, the reduction of overuse, and the misuse of antimicrobials in human and veterinary medicine are the cornerstones required to prevent the spreading of resistant bacteria. Purified drinking water and strongly improved sanitation even in remote areas would prevent the pollution from inadequate treatment of industrial, residential, and farm waste, as all these situations are expanding the resistome in the environment. The One Health concept addresses the interconnected relationships between human, animal, and environmental health as a whole: several countries and international agencies have now included a One Health Approach within their action plans to address AMR. Improved antimicrobial usage, coupled with regulation and policy, as well as integrated surveillance, infection control and prevention, along with antimicrobial stewardship, sanitation, and animal husbandry should all be integrated parts of any new action plan targeted to tackle AMR on the Earth. Since AMR is found in bacteria from humans, animals, and in the environment, we briefly summarize herein the current concepts of One Health as a global challenge to enable the continued use of antibiotics.

show abstract

“…21 Moreover, a KPC-3 producing Escherichia coli, belonging to the ST167 clone, has been recently isolated from seafood bought from a Tunisian retail market. 31 Besides, another study reported the emergence of OXA-23 producing Acinetobacter baumannii ST2 in seafood collected from Bizerte lagoon that was contaminated by hospital effluents. 32 This wide spread of OXA-23 producing clone has been recently detected among two fish belonging to the Pagellus acarne species fished in the Mediterranean Sea of Algeria, near Bejaia, which is about 626.3 km away from Bizerte.…”

Section: Carbapenemase Producing Gram-negative Bacteria From Other Ormentioning

confidence: 99%

“…The bla KPC gene was located in a conjugative plasmid belonging to the Inc FII group and inserted in the Tn3-like transposon, identified as Tn4401d and recognized as an active transposon enhancing the rapid spread of KPC encoding gene. 31,37 We cannot be certain that the bla KPC gene is usually harbored by Inc FII plasmid because not all studies determined this. In fact, according to Table 1, only one Escherichia coli isolate was determined to carry the KPC encoding gene in this plasmid replicon type.…”

Section: Genetic Environmentmentioning

confidence: 99%

<p>Carbapenemase Producing Gram-Negative Bacteria in Tunisia: History of Thirteen Years of Challenge</p>

Dziri

Salabi

et al. 2020

IDR

View full text Add to dashboard Cite

The wide spread of multidrug-resistant bacteria, particularly carbapenem-resistant Gram-negative bacteria (CR-GNB), constitutes a major public health threat worldwide, owing to the limited therapeutic options. This review will describe and uncover the Tunisian experience in the challenge against carbapenem resistance. Indeed, we illuminate on the dissemination of CR-GNB in different hospitals, animals, and other natural environments in this country. We resumed the different carbapenemase variants detected from various bacterial species and mapped their regional distribution, basing on Tunisian published data during a period extended from 2006, the date of its first description in Tunisia, to February 2019. We also resumed the different mobile genetic elements implicated in their dissemination. This review shows that the majority of the research reports focused in the north and the coastal cities in spite of the fact that KPC and IMP carbapenemases were uncommonly detected in our country. However, VIM, NDM-1, and OXA-48 enzymes were usually reported with the predominance of OXA-48 among Enterobacteriaceae. Furthermore, OXA-23, OXA-51, and OXA-58 carbapenemases constituted the main mechanism conferring carbapenem resistance among Acinetobacter baumannii in Tunisia. Collaborative efforts and raising awareness of the threat of antibiotic resistance are required in order to minimize the spread of multidrug-resistant bacteria.

show abstract

KPC-3-producing ST167 Escherichia coli from mussels bought at a retail market in Tunisia

Cited by 27 publications

References 10 publications

Comparative Genome Analysis of an Extensively Drug-Resistant Isolate of Avian Sequence Type 167 Escherichia coli Strain Sanji with Novel In Silico Serotype O89b:H9

Comparative Genome Analysis of an Extensively Drug-Resistant Isolate of Avian Sequence Type 167 Escherichia coli Strain Sanji with Novel In Silico Serotype O89b:H9

Country Income Is Only One of the Tiles: The Global Journey of Antimicrobial Resistance among Humans, Animals, and Environment

<p>Carbapenemase Producing Gram-Negative Bacteria in Tunisia: History of Thirteen Years of Challenge</p>

Contact Info

Product

Resources

About