KPC-2 carbapenemase-producing Pseudomonas aeruginosa reaching Germany

Hagemann, Jürgen Benjamin; Pfennigwerth, Niels; Gatermann, Sören; Baum, Heike von; Essig, Andreas

doi:10.1093/jac/dky105

Cited by 31 publications

(30 citation statements)

References 18 publications

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“…Osolates were generally resistant to aztreonam and/or cefepime or ceftazidime, with the exception of one isolate of P. putida, which was resistant to the carbapenems, imipenem and meropenem. Resistance to carbapenem antibiotics has previously been reported in P. aeruginosa (Voor On 't Holt et al, 2014;Hagemann et al, 2018) and has also been described in P. putida isolated from from clinical samples (Lee et al, 2002). The KPC (Klebsiella pneumoniae carbapenemase enzyme) phenotype of this P. putida isolate was confirmed by chromogenic agar inoculation (Chromagar® KPC).…”

Section: Resultssupporting

confidence: 63%

Pseudomonas SP. in uninspected raw goat’s milk in Rio de Janeiro, Brazil

Ramos

Nascimento

2020

Food Sci. Technol

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Although quite controversial from the point of view of food safety, a considerable number of people across different age and socioeconomic groups consume raw goat's milk. Even if refrigerated after milking, raw goat's milk may still be susceptible to the lipolytic and proteolytic actions of psychrotrophic microorganisms, such as Pseudomonas. On this study, Pseudomonas sp. from 21 refrigerated raw goat's milk samples were characterized. All samples were freely commercialized, without any type of inspection, by small producers and markets from different regions of Rio de Janeiro, Brazil. A total of 136 isolates were identified, most of which belonged to the species, P. putida, P. koreensis, P. monteilii, and P. fluorescens. The production of protease and lipase varied according to the incubation temperature. We found that 91.4% of the Pseudomonas isolates were susceptible to all antibiotics tested. None of the isolates exhibited a multidrug-resistance phenotype. None of the raw goat's milk samples contained detectable antibiotic residues, which may explain the very low frequency of resistant bacteria. Although Pseudomonas is not considered a typical food-related pathogen, its presence indicate that the determination of microbiological standards for raw goat's milk and greater control of the commercialization of this product, are necessary.

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Section: Resultssupporting

confidence: 63%

Pseudomonas SP. in uninspected raw goat’s milk in Rio de Janeiro, Brazil

Ramos

Nascimento

2020

Food Sci. Technol

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“…Our MBL‐producing P. aeruginosa isolates were typed into IMP‐1, IMP‐7 or VIM‐1, and those with IMP‐1 were first discovered worldwide in the late 1980s and spread to Japan in 1991 . To date, the isolates with IMP‐1 have mainly spread in Asian regions .…”

Section: Discussionmentioning

confidence: 99%

“…Our MBL-producing P. aeruginosa isolates were typed into IMP-1, IMP-7 or VIM-1, and those with IMP-1 were first discovered worldwide in the late 1980s and spread to Japan in 1991. 4,6 To date, the isolates with IMP-1 have mainly spread in Asian regions. 5,23 P. aeruginosa with VIM-1 or IMP-7 was also first found in 1999 or 2002, respectively, and has been found in Asian regions (isolated in Japan in 2010) and European regions as well.…”

Section: Discussionmentioning

confidence: 99%

“…4,5 Whereas, KPC and OXA types as other types of carbapenemase are rarely detected in P. aeruginosa. 6 Previous Japanese studies have reported that the most prevalent type of MBL-producing P. aeruginosa was the IMP type, particularly the IMP-1 type. 5 Several molecular epidemiological techniques are required in order to monitor whether MBL-producing P. aeruginosa is clonally spread or not.…”

Section: Introductionmentioning

confidence: 99%

“…MBLs found from P. aeruginosa are mainly typed as the IMP type ( bla IMP‐1 and bla IMP‐2 ) and VIM type ( bla VIM‐1 and bla VIM‐2 ) . Whereas, KPC and OXA types as other types of carbapenemase are rarely detected in P. aeruginosa . Previous Japanese studies have reported that the most prevalent type of MBL‐producing P. aeruginosa was the IMP type, particularly the IMP‐1 type …”

Section: Introductionmentioning

confidence: 99%

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Molecular characteristics of carbapenem‐resistant Pseudomonas aeruginosa isolated from urine in Hyogo, Japan

et al. 2018

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Objectives To investigate the molecular characteristics and epidemiology of metallo‐β‐lactamase‐producing Pseudomonas aeruginosa from urine of urinary tract infection patients in Hyogo Prefecture, Japan. Methods Carbapenem‐resistant P. aeruginosa isolated from the urine of 21 urinary tract infection patients in three general hospitals in Hyogo Prefecture (Japan) were collected between 2007 and 2014. Their antibiotic susceptibilities, metallo‐β‐lactamase screening test, metallo‐β‐lactamase gene sequencing, multilocus sequence typing and repetitive‐sequence‐based polymerase chain reaction were determined for epidemiological analyses to investigate the genetic characteristics. Results Out of 21 isolates, 13 (61.9%) were positive for metallo‐β‐lactamase. There were 11 (52.4%) isolates with IMP‐1 in them, one (4.5%) isolate with IMP‐7 and one (4.5%) isolate with VIM‐1. Metallo‐β‐lactamase‐positive isolates were mainly identified as ST235, and metallo‐β‐lactamase‐negative isolates were STs 357, 277, 234, 439 and 639. Repetitive‐sequence‐based polymerase chain reaction showed metallo‐β‐lactamase‐positive isolates were grouped in eight clusters, and ST235 isolates with IMP‐1 from three hospitals belonging to the identical group I, the other ST235 isolates with IMP‐7 and VIM‐1 were from two hospitals belonging to group II. Conclusions Metallo‐β‐lactamase‐positive P. aeruginosa of ST235 isolates with IPM‐1 were mainly identified from the urine of urinary tract infection patients in Hyogo, Japan. A ST235 isolate with VIM‐1 was found for the first time. Further investigation is necessary to follow the spread of metallo‐β‐lactamase‐positive isolates.

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