Molecular characteristics of carbapenem‐resistant Pseudomonas aeruginosa isolated from urine in Hyogo, Japan

Abstract

Objectives To investigate the molecular characteristics and epidemiology of metallo‐β‐lactamase‐producing Pseudomonas aeruginosa from urine of urinary tract infection patients in Hyogo Prefecture, Japan. Methods Carbapenem‐resistant P. aeruginosa isolated from the urine of 21 urinary tract infection patients in three general hospitals in Hyogo Prefecture (Japan) were collected between 2007 and 2014. Their antibiotic susceptibilities, metallo‐β‐lactamase screening test, metallo‐β‐lactamase gene sequencing, multilocus sequence typing and repetitive‐sequence‐based polymerase chain reaction were determined for epidemiological analyses to investigate the genetic characteristics. Results Out of 21 isolates, 13 (61.9%) were positive for metallo‐β‐lactamase. There were 11 (52.4%) isolates with IMP‐1 in them, one (4.5%) isolate with IMP‐7 and one (4.5%) isolate with VIM‐1. Metallo‐β‐lactamase‐positive isolates were mainly identified as ST235, and metallo‐β‐lactamase‐negative isolates were STs 357, 277, 234, 439 and 639. Repetitive‐sequence‐based polymerase chain reaction showed metallo‐β‐lactamase‐positive isolates were grouped in eight clusters, and ST235 isolates with IMP‐1 from three hospitals belonging to the identical group I, the other ST235 isolates with IMP‐7 and VIM‐1 were from two hospitals belonging to group II. Conclusions Metallo‐β‐lactamase‐positive P. aeruginosa of ST235 isolates with IPM‐1 were mainly identified from the urine of urinary tract infection patients in Hyogo, Japan. A ST235 isolate with VIM‐1 was found for the first time. Further investigation is necessary to follow the spread of metallo‐β‐lactamase‐positive isolates.

Characterize carbapenem-resistant Klebsiella pneumoniae isolates for nosocomial pneumonia and their Gram-negative bacteria neighbors in the respiratory tract

“…And also, the differences in the main type of carbapenemases production among resistant bacteria should be considered. The most prevalent carbapenemases production of P. aeruginosa was MBLs [41,42], particularly IMP, which had significantly higher resistance to most antibiotics [41]. Due to evidence of cooperation or competition between bacteria was so few, our understanding about how relationships between different bacterial species occur still remains confusion.…”

Epidemiology, Molecular Characteristics, and Virulence Factors of Carbapenem-Resistant Pseudomonas aeruginosa Isolated from Patients with Urinary Tract Infections

“…An understanding of the epidemiology with molecular characterization of carbapenemase genes is critical to characterize the genetic diversity for confirming clonal diffusion of CRPA and to provide strategies and infection control measures. 20 P. aeruginosa clones such as sequence types (STs) ST235, ST111, ST233, ST244, ST357, ST308, ST175, ST277, ST654, and ST298, so-called “international” or “high risk” clones, have been spread worldwide and are frequently associated with multidrug resistance. 21 However, the molecular epidemiology and distribution of carbapenemases in CRPA may vary between diverse geographical and clinical origins, and studies on the coexistence of multidrug resistance (MDR) with multiple virulence factors of CRPA isolates causing UTIs are limited.…”

Phenotypic and Genotypic within-host Diversity of Pseudomonas Aeruginosa Urinary Isolates

“…Some of them were mainly associated with multiresistance, including the three most predominant worldwide epidemic high-risk clones (ST111, ST175, ST235) 7 . Of note, ST234 and ST235, previously described for P. aeruginosa urinary isolates 11,12 , were identified for some of our isolates. Within-host diversity was then assessed by comparing phenotypes and genotypes of isolates collected from a given sample.…”