Koyun Bronkopnömonilerinde Mannheimia haemolytica’nın İmmunohistokimyasal ve Bakteriyolojik İncelenmesi

Abstract

Mannheimia haemolytica infection is one of the most common etiologic agents of sheep pneumonia almost all over the world. Ovine pneumonic Mannheimiosis is characterized by severe fibrinous pleuropneumonia. Subacute to chronic cases progress to purulent bronchopneumonia and its squeals include abscessation and fibrous pleural adhesions. In the present study, lungs of 8986 sheep were inspected grossly in the Ahvaz abattoir and totally 65 lungs with visible signs of bronchopneumonia were selected for pathological and bacteriological examinations. Mannheimia haemolytica antigens were detected in 63.07% of immunoperoxidase stained tissue sections while 52.30% of the lungs were positive in bacteriological culture. Suppurative, necrotic and fibrinous types of bronchopneumonia were the most abundant lesions and right cranial lobes, specifically their cranial portions, were the most affected areas. McNemar test showed a significant difference between the diagnostic power of immunohistochemistry (IHC) and bacterial culture in detection of M. haemolytica (κ=0.66). Considering IHC as a golden test, sensitivity and specificity of bacterial culture were estimated as 78.05 and 91.67%, respectively. Chi-squared test showed significant correlations between the distribution of the lesions and bacterial isolation (P=0.04), types of lesions and IHC results (P=0.01), and also types of bronchopneumonia and mixed/pure isolation (P=0.008). This study showed the significant role of Mannheimia haemolytica in causing pneumonic lesions of studied sheep. Keywords

Kars İlinde Pnömonili Koyunlarda Mannheimia spp. ve Pasteurella spp.’nin İmmunohistokimyasal ve Moleküler Tanımlanması

“…In tissue sections, positive immunoreactivity against bacterial antigens was observed in the cytoplasm of leucocytes, pneumocytes, bronchial and bronchiolar epithelial cells, epithelial cells fallen off into the lumens of bronchia and bronchioles, around the necrotic areas, and among the oat cells. This immunolocalization pattern was in accordance with the previous investigations [25,[27][28][29] .…”

“…In a similar study performed by Bemani et al [29] in Iran, 63.7% positivity was determined in sheep with bronchopneumonia. In this study, positive immunoreactivity against the bacterial antigens was reported in the surface and cytoplasm of epithelial cells, and the cytoplasm of macrophages, leucocytes, and the bronchial exudate.…”

“…On the other hand PCR was suggested to be more sensitive, specific and faster method compared to the other conventional techniques [8,24] . Immunohistochemical detection of the bacterial agents in sections prepared from paraffin tissue blocks was also suggested to be quite successful [25][26][27][28][29] .…”