Isolation of the various serotypes of Mannheimia haemolytica and preparation of the first vaccine candidate in Iran

Molaee, Hajar; Tahamtan, Yahya; Saeednezhad, Ehlam; Hayati, Masoumeh

doi:10.1007/s11033-022-07890-4

Cited by 2 publications

(2 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…haemolytica was isolated from a nasal swab of Hainan black goat and named Mh-HN0721, which was preserved in our laboratory. Single colonies were then selected from Tryptic Soya Agar (TSA) medium (Qingdao Haibo Biotechnology Co., Ltd., Qingdao, China) for serotyping of Mh-HN0721 (Table 1) [28,29]. The PCR reaction system was 50 µL, consisting of 25 µL Taq Master mix, 2 µL upstream primer, 2 µL downstream primer, and 21 µL ddH 2 O.…”

Section: Bacteria Source and Serotypes Identificationmentioning

confidence: 99%

Immune Cells in the Spleen of Mice Mediate the Inflammatory Response Induced by Mannheimia haemolytica A2 Serotype

Jiao,

Jiang,

Meng

et al. 2024

Animals

View full text Add to dashboard Cite

(1) Background: Mannheimia haemolytica (M. haemolytica) is an opportunistic pathogen and is mainly associated with respiratory diseases in cattle, sheep, and goats. (2) Methods: In this study, a mouse infection model was established using a M. haemolytica strain isolated from goats. Histopathological observations were conducted on various organs of the mice, and bacterial load determination and RNA-seq analysis were specifically performed on the spleens of the mice. (3) Results: The findings of this study suggest that chemokines, potentially present in the spleen of mice following a M. haemolytica challenge, may induce the migration of leukocytes to the spleen and suppress the release of pro-inflammatory factors through a negative feedback regulation mechanism. Additionally, an interesting observation was made regarding the potential of hematopoietic stem/progenitor cells congregating in the spleen to differentiate into immune cells, which could potentially collaborate with leukocytes in their efforts to counteract M. haemolytica invasion. (4) Conclusions: This study revealed the immune regulation mechanism induced by M. haemolytica in the mouse spleen, providing valuable insights into host–pathogen interactions and offering a theoretical basis for the prevention, control, and treatment of mannheimiosis.

show abstract

Section: Bacteria Source and Serotypes Identificationmentioning

confidence: 99%

Immune Cells in the Spleen of Mice Mediate the Inflammatory Response Induced by Mannheimia haemolytica A2 Serotype

Jiao,

Jiang,

Meng

et al. 2024

Animals

View full text Add to dashboard Cite

show abstract

“…Bovine respiratory disease complex (BRDC) is a wellknown disease causing huge economic losses for both cattle feedlot because of high mortality rates, expensive costs of prevention, treatment and low carcass value (Confer 2009;Fulton 2009;Mcvey 2009;Mohiuddin et al 2022). In North American cattle industry, BRD induced were assessed to be about one billion dollars annually (Molaee 2022;Miles 2009). Two members of the Family Pasteurellaceae, namely, Pasteurella multocida and Mannheimia haemolytica serve as a main bacterial causative pathogen as cattle respiratory disease, which are highly accompanied by BRD (Rice et al 2007;Griffin et al 2010;Imran et al 2022;Valeris-Chacin 2022).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Pasteurella Multocida and Mannheimia Haemolytica Infections in Cattle Using Lateral Flow Immunochromatographic Assay

2023

Int J Vet Sci

View full text Add to dashboard Cite

This study was mainly designed to advance and recover the control measures of Bovine respiratory disease (BRD) via accelerating the field identification of some BRD causing pathogens. A simple rapid field test was developed mainly to identify Pasteurella multocida and Mannheimia haemolytica, both are highly associated with BRD. A full identified Pasteurella multocida and Mannheimia haemolytica cultures were earned from Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Egypt. Species-specific bacterial antigen of outer membrane protein of each bacterial strain was prepared separately for M. haemolytica and P. multocida. Specific polyclonal antibodies were prepared by amplifying each one in guinea pigs and rabbits. Pathogens-specific lgG were purified using caprylic acid technique. Pathogens-specific lgG titers were measured by ELISA. The colloidal gold nanoparticles were conjugated to the rabbit mono specific polyclonal lgG antibody and did not conjugate to guinea pig specific polyclonal lgG. The particles were then utilized to develop the Lateral flow kits (LFK) which is a rapid assay for detection for P. multocida and M. haemolytica in clinical samples when Compared to the conventional Bacteriological tests. Results: Percentage of accuracy, specificity and sensitivity of LFK kits recorded 93, 94.4 and 90.4%, respectively, for P. multocida and 91.5, 94.3and 84.7%, respectively, for Mannheimia haemolytica.

show abstract

Isolation of the various serotypes of Mannheimia haemolytica and preparation of the first vaccine candidate in Iran

Cited by 2 publications

References 43 publications

Immune Cells in the Spleen of Mice Mediate the Inflammatory Response Induced by Mannheimia haemolytica A2 Serotype

Immune Cells in the Spleen of Mice Mediate the Inflammatory Response Induced by Mannheimia haemolytica A2 Serotype

Diagnosis of Pasteurella Multocida and Mannheimia Haemolytica Infections in Cattle Using Lateral Flow Immunochromatographic Assay

Contact Info

Product

Resources

About