Korea National Stem Cell Bank

Kim, Jung-Hyun; Jo, Hye‐Yeong; Ha, Hye-Yeong; Kim, Yong-Ou

doi:10.1016/j.scr.2021.102270

Cited by 15 publications

(11 citation statements)

References 6 publications

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“…Here, we established a standard operating protocol for a reproducible method to generate functional liver organoids with the combination of 2D and 3D differentiation strategies. To increase the utility of the organoids, through the Korea National Stem Cell Bank [19], we are providing the starting materials together with the protocol for dissemination. j-organoid.org…”

Section: Discussionmentioning

confidence: 99%

“…This protocol resulted in the generation of mature hepatic organoids that can be applied in suitable hepatic models for drug toxicity, drug screening, and disease modeling. Furthermore, we are making the starting materials (KSCBi005) [19] available for broad distribution; the differentiation capability of this material was confirmed via the Korea National Stem Cell Bank (https:// nih.go.kr/contents.es?mid=a50401110200).…”

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confidence: 99%

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Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells

et al. 2022

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Mature liver organoids are promising cell sources for research to understand the pathology underlying a variety of conditions affecting the liver, including end-stage chronic liver disease. Although several methods exist for the differentiation of mature hepatic organoids derived from human induced pluripotent stem cells (hiPSCs), organoid generation can fail due to various experimental culture conditions. Therefore, we established a standard operating protocol for generating mature and expandable hepatic organoids derived from hiPSCs, and we made the starting materials available to facilitate the wide use of the protocol.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Standard operating protocol of hepatic organoid differentiation from human induced pluripotent stem cells

et al. 2022

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“…hESC H9 (WiCell) was maintained on a Matrigel-coated 35-mm dish using mTeSR 1 (STEMCELL), and the medium was replenished every day. ThehiPSC line CMC-hiPSC-003 was obtained from Korea National Stem Cell Bank ( Kim et al, 2021 ) and maintained on a vitronectin-coated 35 mm dish using E8 media (STEMCELL). When colonies of both H9 or CMC-hiPSC-003 grew to approximately 500 μm in diameter, cellular differentiation was induced using mesoderm differentiation medium (APEL 2 supplemented with 1X insulin-transferrin-selenium-X [Invitrogen] and 100 ng/ml BMP4).…”

Section: Methodsmentioning

confidence: 99%

Single-Cell RNA Sequencing of Human Pluripotent Stem Cell-Derived Macrophages for Quality Control of The Cell Therapy Product

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Gil

et al. 2022

Front. Genet.

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Macrophages exhibit high plasticity to achieve their roles in maintaining tissue homeostasis, innate immunity, tissue repair and regeneration. Therefore, macrophages are being evaluated for cell-based therapeutics against inflammatory disorders and cancer. To overcome the limitation related to expansion of primary macrophages and cell numbers, human pluripotent stem cell (hPSC)-derived macrophages are considered as an alternative source of primary macrophages for clinical application. However, the quality of hPSC-derived macrophages with respect to the biological homogeneity remains still unclear. We previously reported a technique to produce hPSC-derived macrophages referred to as iMACs, which is amenable for scale-up. In this study, we have evaluated the biological homogeneity of the iMACs using a transcriptome dataset of 6,230 iMACs obtained by single-cell RNA sequencing. The dataset provides a valuable genomic profile for understanding the molecular characteristics of hPSC-derived macrophage cells and provide a measurement of transcriptomic homogeneity. Our study highlights the usefulness of single cell RNA-seq data in quality control of the cell-based therapy products.

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“…Xu et al generated HLA-C-retained but HLA-A&B-disrupted immunocompatible iPSCs, 12 lines of which along with HLA-class Ⅱ knockout were suggested to cover> 90% of the global population in terms of immunological compatibility [123] . The Korea National Stem Cell Bank has preserved 22 Good Manufacturing Practice-compliant homozygous HLA-type iPSC lines that cover HLA haplotype compatibility for 51% of the Korean population [124] . Eighteen kinds of quality tests including identity, sterility, consistency, stability, and safety had been performed to ensure the quality of the stocking cell lines.…”

Section: Cell Banking Of Ipscs To Improve Efficiencymentioning

confidence: 99%