Konservasi In Vitro Tanaman Hias Akuatik Bacopa australis dan Alternanthera reineckii Menggunakan Paklobutrazol dan Benzil Adenin

Lestari, Endang Gati; Nugraha, Media Fitri Isma; Yunita, Rossa; Azizi, Alfia Annur Aini

doi:10.21082/blpn.v27n1.2021.p11-20

Cited by 1 publication

(3 citation statements)

References 12 publications

(17 reference statements)

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“…Conservation of aquatic ornamental plants B. australis and Alternatia reinecki using MS media + 0.7 mg/L paclobutrazol showed inhibition until the 6 th on shoot height, number of shoots, number of roots, root length, and number of leaves. In this conservation medium, the culture remains green and looks fresh (Lestari et al 2021). In culture conservation, through in vitro culture, several techniques can be applied, including cryopreservation, simple conservation, and slow growth conservation (Dewi et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Paclobutrazol is an active compound inhibiting the oxidation of kaurene to ent-kaurene as a precursor of the growth regulator of gibberellic acid in the apical meristem, causing inhibition of cell division at the growth point (Negi et al 2017;Bisht et al 2018). In slow-growth conservation, growth and cell division are conditioned to occur very slowly, or metabolism is stopped so that culture development stops and does not change the genetic nature of the plant (Lestari et al 2021). The benefits of in vitro culture conservation include keeping germplasm accessions/collections from becoming extinct (Huang et al 2014) by inhibiting cell growth and division from becoming very slow (Indrayanti et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Formulas media for the propagation of aquatic ornamental plants are still limited, namely B. australis (Nugraha et al 2017;Yunita et al 2018), as well as formula media for in vitro culture conservation. Lestari et al (2021) conducted a conservation study through in vitro culture of B. australis and A. reinecki using MS + paclobutrazol media. So many aquatic plants are currently being developed that research is needed to obtain formula media for in vitro propagation and conservation.…”

Section: Introductionmentioning

confidence: 99%

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The Formula media in vitro Propagation and Conservation of Ludwigia sp.

Lestari¹,

Nugraha²,

Yunita³

2023

J.Tropical Biodiversity Biotechnology

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The aquatic plant "Red Malang” (Ludwigia sp.) has a fairly high economic value as an ornamental aquatic plant, so it has the potential to be developed. The growth of in vitro cultures in culture bottles is high-speed, so it is necessary to find a formula media to inhibit growth so that the frequency of subcultures is reduced. The current research aims to produce a formula media for shoot multiplication and in vitro culture conservation. The research was carried out at the ICABIOGRAD tissue culture laboratory from April 2020 to June 2021. Research activities included plant propagation, conservation, and regeneration after conservation. Plant material was using in the form of a culture collection in the ICABIOGRAD tissue culture laboratory, treatment media for propagation were BA (0; 0.1; 0.3; 0.5; 0.7 and 0.9 mg/L) + thidiazuron (TDZ) (0 and 0.1mg/L). For conservation were MS + BA medium (0 and 0.1 mg/L) + paclobutrazol (0; 0.1; 0.3; 0.5; 0.7 mg/L) and for shoot regeneration after conservation using MS medium without Plant Growth Regulator (PGR). Data analysis using the Anova SAS version 9.0 test program. Further test using DMRT test with alpha level 5%. There was no difference in the mean value between levels of TDZ treatment on the number of shoots and leaves. The difference in the mean value between levels of TDZ treatment was very significant on shoot height, the number of roots, and root length. BA treatment with a concentration of 0.7 mg/L is better because it gives higher results for each observation variable. For conservation, treatment with paclobutrazol 0.5 mg/L inhibited shoot and leaf count, and 0.3 mg/L inhibited shoot formation. Cultures stored for six months grew normally after being regenerated. The highest shoots and the highest number of leaves were obtained from the treatment of paclobutrazol without BA. This study indicated that the propagation media of aquatic plants Ludwigia sp. did not require high concentrations of BA. Cultures could be stored for over six months using paclobutrazol with 0.3-0.6 mg/L.

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Section: Introductionmentioning

confidence: 99%