Komagataeibacter diospyri sp. nov., a novel species of thermotolerant bacterial nanocellulose-producing bacterium

Naloka, Kallayanee; Yukphan, Pattaraporn; Matsutani, Minenosuke; Matsushita, Kazunobu; Theeragool, Gunjana

doi:10.1099/ijsem.0.003745

Cited by 15 publications

(12 citation statements)

References 49 publications

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Section: Introductionmentioning

confidence: 99%

“…One way to overcome this problem relies on the detailed characterization and classification of these strains based on the use of robust and reliable genomic and molecular markers, which are now used in order to ascertain taxonomical classifications at both genus and species levels [25,26]. While recent studies have addressed the genomic characterization of several Komagataeibacter strains and unveiled several of their genetic properties and metabolic features [8,18,[27][28][29][30][31][32][33][34][35][36], most of these works have focused on a reduced number of strains, limiting the general study of (i) Komagataeibacter phylogeny and (ii) the evolution of cellulose synthase genes.…”

Section: Introductionmentioning

confidence: 99%

“…The genus Komagataeibacter currently comprises 18 different independent species: Komagataeibacter cocois [7], Komagataeibacter diospyri [8], Komagataeibacter europaeus [9], Komagataeibacter hansenii [10], Komagataeibacter intermedius [11], Komagataeibacter kakiaceti [12], Komagataeibacter maltaceti [13], Komagataeibacter medellinensis [14], Komagataeibacter melaceti [15], Komagataeibacter melomenusus [15], Komagataeibacter nataicola [16], Komagataeibacter oboediens [17], Komagataeibacter pomaceti [18], Komagataeibacter rhaeticus [19], Komagataeibacter saccharivorans [16], Komagataeibacter sucrofermentans [20], Komagataeibacter swingsii [19] and Komagataeibacter xylinus [21] , which is the type species of the genus. In addition, the Gluconacetobacter entanii species [22] also present similar characteristics to those of Komagataeibacter .…”

Section: Introductionmentioning

confidence: 99%

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Phylogenomic and comparative analyses support the reclassification of several Komagataeibacter species as novel members of the Novacetimonas gen. nov. and bring new insights into the evolution of cellulose synthase genes

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Crespo

Nascimento

2022

International Journal of Systematic and Evolutionary Microbiology

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The genus Komagataeibacter harbours bacteria presenting the ability to produce increased levels of crystalline nanocellulose, as well as strains used in the industrial production of fermented products and beverages. Still, most of the studies of this biotechnologically relevant genus were conducted based on limited phenotypic methodologies and taxonomical classifications. In this work, a detailed analysis of the currently described genus Komagataeibacter was conducted based on phylogenomic analysis, unveiling the phylogenomic relationships within the genus and allowing a detailed phylogenetic analysis of biotechnologically important genes such as those involved in cellulose biosynthesis (bcs genes). Phylogenomic and comparative genomic analysis revealed that several type strains formed an independent genomic group from those of other Komagataeibacter , prompting their reclassification as members of a novel genus, hereby termed Novacetimonas gen. nov. The results support the reclassification of Komagataeibacter hansenii , Komagataeibacter cocois , Komagataeibacter maltaceti and Komagataeibacter pomaceti as novel members of the genus Novacetimonas. The Novacetimonas hansenii species is the proposed representative of the novel genus. Importantly, phylogenetic analysis based on cellulose biosynthesis genes (bcsABCD, bcsAB2XYC2, bcsAB3C3, bcsAB4), showed that the evolutionary history of these genes is closely related to the strain’s phylogenomic/taxonomic classification. Hence, the robust taxonomic classification of these bacteria will allow the better characterization and selection of strains for biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenomic and comparative analyses support the reclassification of several Komagataeibacter species as novel members of the Novacetimonas gen. nov. and bring new insights into the evolution of cellulose synthase genes

Brandão

Crespo

Nascimento

2022

International Journal of Systematic and Evolutionary Microbiology

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“…nov. isolated from apple cider vinegar also indicate that these new novel strains have the ability to utilize ammoniacal nitrogen in Hoyer-Frateur medium and Asai medium with D-glucose, D-mannitol and only K. melaceti can utilize with ethanol in Hoyer-Frateur medium (Maric et al, 2020). Naloka et al (2020) revealed in their study on new novel species of a nano cellulose-producing bacterium with thermotolerant characteristics isolated from persimmon and sapodilla fruits with proposed named K. dispyri sp. nov. Like other cellulose-producing bacteria, K. dispyri can grow without acetic acid and on 30% D-glucose and also able to utilize and form acid from carbon sources such as raffinose and sucrose.…”

Section: Komagataeibacter Spmentioning

confidence: 93%

Employed Bacterial Species and Bacterial Cellulose (BC) Applications: The State of Play

Aziz¹,

Adnan

2022

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Bacterial cellulose (BC) is an uprising bio-polymer produced by various bacterial strains, which is infamous for its prominent biological properties and applications. Receiving ample attention due to those unique properties, various genus and bacterial strains have been used for BC biosynthesis and the studies of its production have been recorded throughout the years. Although a lot of research and implementation has been done on BC, studies in the search for low-cost, effective medium contributing to higher BC yields were still in continuation to this day. This review article overviews the employed bacterial strains and their recent advance, modified, and low-cost medium in the development of BC composites. Special emphasis is placed on the new-novel strains for BC production and BC applications. Compilations of literature were compiled to outline the sources and also findings by previous and recent researchers. It was found that numerous studies have attempted to enhance BC production, which includes the utilization of various bacterial strains to fulfill industrial needs. Hence, this review comprises bacterial genera and species, which are mainly used in the production of BC such as Komagataeibacter, Gluconobacter, Gluconacetobacter, Enterobacter, and Pseudomonas.The recent studies enforced on BC focusing on higher production and the application of BC on an industrial scale will also be reviewed.

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“…The main phenotypic features which differentiate the genus Komagataeibacter from Gluconacetobacter are absence of flagellation, absence of water-soluble brown pigment production when grown on glucose-yeast-calcium-carbonate medium, and absence of 2,5-diketo-D-glucose production from glucose [3,5]. As accessed on 28 May 2020 from the website of List of Prokaryotic names with Standing in Nomenclature (LPSN) [6], the genus Komagataeibacter contains 17 species which were isolated from various sugar and alcohol containing substrates, such as vinegars, fruits, kombucha, coconut milk, nata de coco, and beet juice [7][8][9][10][11][12][13]. The genus Komagataeibacter gained immense interest among researchers due to the massive and efficient production of nanocellulose by members of the species Komagataeibacter xylinus, Komagataeibacter medellinensis, Komagataeibacter hansenii, Komagataeibacter nataicola, Komagataeibacter oboediens, Komagataeibacter rhaeticus, Komagataeibacter saccharivorans and Komagataeibacter pomaceti [14].…”

Section: Introductionmentioning

confidence: 99%

Description of Komagataeibacter melaceti sp. nov. and Komagataeibacter melomenusus sp. nov. Isolated from Apple Cider Vinegar

et al. 2020

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Two novel strains AV382 and AV436 were isolated from a submerged industrial bioreactor for production of apple cider vinegar in Kopivnik (Slovenia). Both strains showed very high (≥98.2%) 16S rRNA gene sequence similarities with Komagataeibacter species, but lower 16S–23S rRNA gene internal transcribed spacer (ITS). The highest similarity of the 16S–23S rRNA gene ITS of AV382 was to Komagataeibacter kakiaceti LMG 26206T (91.6%), of AV436 to Komagataeibacter xylinus LMG 1515T (93.9%). The analysis of genome sequences confirmed that AV382 is the most closely related to K. kakiaceti (ANIb 88.2%) and AV436 to K. xylinus (ANIb 91.6%). Genome to genome distance calculations exhibit for both strains ≤47.3% similarity to all type strains of the genus Komagataeibacter. The strain AV382 can be differentiated from its closest relatives K. kakiaceti and Komagataeibacter saccharivorans by its ability to form 2-keto and 5-keto-D-gluconic acids from glucose, incapability to grow in the presence of 30% glucose, formation of C19:0 cyclo ω8c fatty acid and tolerance of up to 5% acetic acid in the presence of ethanol. The strain AV436 can be differentiated from its closest relatives K. xylinus, Komagataeibacter sucrofermentans, and Komagataeibacter nataicola by its ability to form 5-keto-D-gluconic acid, growth on 1-propanol, efficient synthesis of cellulose, and tolerance to up to 5% acetic acid in the presence ethanol. The major fatty acid of both strains is C18:1ω7c. Based on a combination of phenotypic, chemotaxonomic and phylogenetic features, the strains AV382T and AV436T represent novel species of the genus Komagataeibacter, for which the names Komagataeibactermelaceti sp. nov. and Komagataeibacter melomenusus are proposed, respectively. The type strain of Komagataeibacter melaceti is AV382T (= ZIM B1054T = LMG 31303T = CCM 8958T) and of Komagataeibacter melomenusus AV436T (= ZIM B1056T = LMG 31304T = CCM 8959T).

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Komagataeibacter diospyri sp. nov., a novel species of thermotolerant bacterial nanocellulose-producing bacterium

Cited by 15 publications

References 49 publications

Phylogenomic and comparative analyses support the reclassification of several Komagataeibacter species as novel members of the Novacetimonas gen. nov. and bring new insights into the evolution of cellulose synthase genes

Phylogenomic and comparative analyses support the reclassification of several Komagataeibacter species as novel members of the Novacetimonas gen. nov. and bring new insights into the evolution of cellulose synthase genes

Employed Bacterial Species and Bacterial Cellulose (BC) Applications: The State of Play

Description of Komagataeibacter melaceti sp. nov. and Komagataeibacter melomenusus sp. nov. Isolated from Apple Cider Vinegar

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