Description of Komagataeibacter melaceti sp. nov. and Komagataeibacter melomenusus sp. nov. Isolated from Apple Cider Vinegar

Marič, Leon; Cleenwerck, Ilse; Accetto, Tomaž; Vandamme, Peter; Trček, Janja

doi:10.3390/microorganisms8081178

Cited by 39 publications

(24 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the capacity of ENS9b to metabolize acetic acid was confirmed by the formation of BC pellicles and its absence in the control cultivation (PY medium without 0.35% acetic acid or 3% ethanol, Figure S3). Similar to other Komagataeibacter spp., K. rhaeticus ENS9b showed positive and negative results for catalase and oxidase tests, respectively; did not require acetic acid for growth, oxidized acetate, and lactate in the PY medium and demonstrated acetic acid overoxidation (Figure S4) [49,50].…”

Section: Characterization and Classification Of The Cellulose-producing Isolatementioning

confidence: 99%

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

et al. 2021

View full text Add to dashboard Cite

Komagataeibacter spp. has been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently, studies have demonstrated the capacity of Komagataeibacter spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in Komagataeibacter spp. In this study, we isolated a cellulose-producing bacterium capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from the biodiesel production process. The isolate, affiliated to Komagataeibacter genus, synthesized cellulose in a minimal medium containing glucose (3.3 ± 0.3 g/L), pure glycerol (2.2 ± 0.1 g/L) and crude glycerol (2.1 ± 0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from the central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when K. rhaeticus ENS9b was grown in a minimal medium supplemented with acetate, BC production was not observed. However, in the presence of readily utilizable substrates, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.

show abstract

Section: Characterization and Classification Of The Cellulose-producing Isolatementioning

confidence: 99%

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Both concentrations are considered high and known resistant bacteria are also only affected by similar concentrations (Fernández et al, 2014). Another study also found that Komagataeibacter melaceti and Komagataeibacter melomenusus are resistant towards chloramphenicol as they possess a homologue for chloramphenicol acetyltransferase which inhibits the antibacterial activity (Marič et al, 2020).…”

Section: Resultsmentioning

confidence: 96%

Development of Selective Media for Komagataeibacter intermedius and Dekkera bruxellensis from a Mixed Culture

Pratama

Devanthi

Kho

2021

Nusantara Science and Technology Proceedings

View full text Add to dashboard Cite

Komagataeibacter intermedius and Dekkera bruxellensis are some of the most commonly found bacteria and yeast, respectively, during Kombucha fermentation. The development of selective media for separating both species is essential to make an accurate cell enumeration. This study aimed to develop a selective media for the enumeration of K. intermedius and D. bruxellensis from a mixed culture sample. Hestrin and Schramm (HS) agar and Potato Dextrose Agar (PDA) were chosen as the enumeration media for K. intermedius and D. bruxellensis, respectively. To select for K. intermedius, HS agar was added with various concentrations of cycloheximide (0, 1, and 10 mg/mL), NaCl (2% and 5%), and acetic acid (1%). To select for D. bruxellensis, PDA was added with various concentrations of chloramphenicol (0, 0.3, and 3 μg/mL) and NaCl (2% and 5%). Each culture (~10 6 log CFU/mL) was serially diluted and plated on the respective agar media, followed by incubation at 30°C for 1-6 days. The results showed that PDA containing 2% NaCl could completely suppress K. intermedius growth while permitting complete recovery of D. bruxellensis. However, it took 6 days until visible growth was observed by D. bruxellensis. Moreover, HS with the addition of 1% acetic acid successfully inhibited D.bruxellensis while allowing the complete recovery of K. intermedius. The findings suggested that the two media were suitable for separating K. intermedius and D. bruxellensis on agar media, thus allowing a more accurate cell counting in studies involving the mixed cultures of both species.

show abstract

“…Similar to other Komagataeibacter spp., K. rhaeticus ENS9b showed positive and negative results for catalase and oxidase tests, respectively, did not require acetic acid for growth, oxidized acetate and lactate in PY medium and demonstrated acetic acid overoxidation (Fig. S4) [49,50].…”

Section: Characterization and Classification Of The Cellulose Producing Isolatementioning

confidence: 99%

Characterization of Komagataeibacter Isolate Reveals New Prospects on Waste Stream Valorization for Bacterial Cellulose Production

Cannazza¹,

Rissanen²,

Guizelini³

et al. 2021

Preprint

View full text Add to dashboard Cite

Komagataeibacter spp. have been used for the bioconversion of industrial wastes and lignocellulosic hydrolysates to bacterial cellulose (BC). Recently studies have demonstrated the capacity of Komagataeibacter spp. in the biotransformation of inhibitors found in lignocellulosic hydrolysates, aromatic lignin-derived monomers (LDMs) and acetate. In general, detoxification and BC synthesis from lignocellulosic inhibitors requires a carbon flow from acetyl-coA towards tricarboxylic acid and gluconeogenesis, respectively. However, the related molecular aspects have not yet been identified in Komagataeibacter spp. In this study, we isolated a cellulose producing bacteria capable of synthesizing BC in a minimal medium containing crude glycerol, a by-product from biodiesel production process. The isolate, affiliated to Komagataeibacter genus, synthesized cellulose in minimal medium containing glucose (3.3±0.3 g/L), pure glycerol (2.2±0.1 g/L) and crude glycerol (2.1±0.1 g/L). Genome assembly and annotation identified four copies of bacterial cellulose synthase operon and genes for redirecting the carbon from central metabolic pathway to gluconeogenesis. According to the genome annotations, a BC production route from acetyl-CoA, a central metabolic intermediate, was hypothesized and was validated using acetate. We identified that when K. rhaeticus ENS9b was grown in minimal medium supplemented with acetate, BC production was not observed. However, in presence of readily utilizable substrate, such as spent yeast hydrolysate, acetate supplementation improved BC synthesis.

show abstract

Description of Komagataeibacter melaceti sp. nov. and Komagataeibacter melomenusus sp. nov. Isolated from Apple Cider Vinegar

Cited by 39 publications

References 76 publications

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

Characterization of Komagataeibacter Isolate Reveals New Prospects in Waste Stream Valorization for Bacterial Cellulose Production

Development of Selective Media for Komagataeibacter intermedius and Dekkera bruxellensis from a Mixed Culture

Characterization of Komagataeibacter Isolate Reveals New Prospects on Waste Stream Valorization for Bacterial Cellulose Production

Contact Info

Product

Resources

About