KOH string and Vancomycin susceptibility test as an alternative method to Gram staining

Dash, Chinmaya; Payyappilli, Rajan J

doi:10.18320/jimd/201603.0288

Cited by 13 publications

(13 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The result of Gram test using 3% Potassium Hydroxide (KOH) shows 68.42% Gram-negative group and 31.57% Gram-positive group (See Table 1). The Gram method using Potassium Hydroxide is easier and more practical and provides a better diagnosis than the Gram staining procedure (Dash and Payyappilli 2016).…”

Section: The Isolation and Characterization Of Endophytic Bacteria Frmentioning

confidence: 99%

The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi

et al. 2020

View full text Add to dashboard Cite

Abstract. Sudewi S, Ala A, Baharuddin, Farid M. 2020. The isolation, characterization endophytic bacteria on from roots of local rice plant Kamba in Central Sulawesi, Indonesia. Biodiversitas 21: 1614-1624. The local Kamba rice plants are cultivated by the Bada Valley people for generations by obtaining seeds from previous crops. This rice plant is able to survive even though it is cultivated with traditional cultivation techniques. The ability to survive is suspected because this local rice plant is associated with endophytic bacteria through a variety of mechanisms including being able to produce the IAA hormone and its ability to dissolve phosphate. This study aims to find endophytic bacteria that have the potential to produce IAA and phosphate solvents through morphological and physiological characterization (Gram reaction test with 3% KOH, catalase test, hypersensitivity reaction and hemolysis activity), screening of IAA-producing bacteria qualitative and quantitative, phosphate dissolution activity on Pikovskaya medium, and quantitative using a spectrophotometer. Endophytic bacteria isolates from Gintu give the highest yields in IAA production and phosphate solubility activity, namely RKGU11 and RKGU6, respectively at 4,905 mg L-1 and 10.984 mg L-1 so that it has the potential to be used as a candidate for biofertilizer agents in developing effective sustainable agriculture, respectively, efficient and environmentally friendly.

show abstract

Section: The Isolation and Characterization Of Endophytic Bacteria Frmentioning

confidence: 99%

The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Gram identification is an important and useful technique needed for the categorization of bacteria into Gram-positive or negative groups (Dash and Payyappilli 2016). The KOH technique is used as an alternative and also considered to be more efficient.…”

Section: Gram Identification By Using 4% Potassium Hydroxide (Koh) Mementioning

confidence: 99%

Diversity of endophytic and rhizosphere bacteria from pineapple (Ananas comosus) plant in semi-arid ecosystem

et al. 2020

View full text Add to dashboard Cite

Abstract. Putrie RFW, Aryantha INP, Iriawati, Antonius S. 2020. Diversity of endophytic and rhizosphere bacteria from pineapple (Ananas comosus) plant in semi-arid ecosystem. Biodiversitas 21: 3084-3093. The natural environment majorly influences plant microbiome diversity, in terms of endophytic and rhizobacteria. Pineapple (Ananas comosus L. Merr) has been identified as one of host plants with a capacity to grow in semiarid ecosystems. The intrinsic microbial symbiont composition is recognized in a specific ecological niche. The scientific information available is limited to the inoculants' ability to reduce plant stress, although the diversity in a semiarid ecosystem has not been studied. The aim of this research is to investigate the diversity of endophytic and rhizosphere bacteria obtained from pineapple in a semiarid ecosystem. A total of 117 culturable microbial isolates, dominated by Gram-positive bacteria were obtained from pineapple (88) and rhizosphere (29). The metagenomic analysis showed higher diversity and abundance in rhizospheres compared to the endophytes. Actinobacterium was identified as the most populated colony, while Proteobacterium was more prominent in the host plant. The Bacillus genera were recognized as the dominant symbiont genus. Therefore, the culturable isolates were further developed as candidate inoculants for microbe databases. This is expected to be useful in broadening theoretical knowledge.

show abstract

“…Gram tests were carried out by using a KOH 3% string test and Gram staining test. KOH 3% string test was done according to Ali et al (2016); Dash and Payyappilli (2016), a loopful of a bacterial colony from the culture plate was emulsified over glass slide in suspension of 3% KOH. Gram staining test was done according to Ayuningrum (2017), using gram's staining solution A (crystal violet), solution B (lugol iodine), solution C (alcohol) and solution D (safranin).…”

Section: Gram Testmentioning

confidence: 99%

Exploration culturable bacterial symbionts of sponges from Ternate Islands, Indonesia

et al. 2019

View full text Add to dashboard Cite

Abstract. Trianto A, Radjasa OK, Sabdono A, Muchlissin SI, Afriyanto R, Sulistiowati, Radjasa SK, Crews P, Mccauley E. 2019. Exploration culturable bacterial symbionts of sponges from Ternate Islands, Indonesia. Biodiversitas 20: 776-782. Multidrug resistance, (MDR) bacteria seriously threaten human health which causes a more difficult and extends the treatment period, and increases the risk of death. Sponges-associated bacteria, a well known for their rich in chemical classes and bioactivities, are the prolific producers of bioactive compounds. In order to search new sources of antibacterial compounds, we collected a total of 55 sponges from Ternate Island that provided 324 bacterial isolates. The data showed that sponges-isolates ratio in the anthropogenic area was relatively higher than the other collection sites. The isolates were screened for the antibacterial activity against Klebsiella pneumoniae-RSDK, K. pneumoniae-UI, Pseudomonas aeruginosa A-UI, P. aeruginosa B-UI, Staphylococcus aureus-UI, MRSA-UI, MRPA-UI, Bacillus subtilis-RSDK, B. subtilis-UI, Salmonella typhi-UI, and MDR E. coli. The isolates were able to inhibit 0-7 the pathogenic bacteria on 24 h and 48 h. The most active bacteria were identified as B. clausii, V. chiguensis, B. tropicus, P. marcusii, B. tropicus, V. parahaemolyticus, B. paramycoides, and V. dokdonensis. In conclusion, the sponges in the anthropogenic affected area have higher bacterial symbionts than that of in the pristine area. In this study, the results of isolation of the symbiont bacteria from sponge samples were obtained and morphologically observed for these bacterial isolates. The number of bacteria that were isolated were 133 bacterial isolates and ten isolates including producing active secondary metabolites

show abstract

KOH string and Vancomycin susceptibility test as an alternative method to Gram staining

Abstract: Abstract:Gram staining is a common technique to differentiate Gram positive and Gram negative bacteria.

Cited by 13 publications

References 6 publications

The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi

The isolation, characterization endophytic bacteria on indole-3-acetic acid-producing and phosphate solubilizing from roots of local rice plant Kamba in Bada Valley, Central Sulawesi

Diversity of endophytic and rhizosphere bacteria from pineapple (Ananas comosus) plant in semi-arid ecosystem

Exploration culturable bacterial symbionts of sponges from Ternate Islands, Indonesia

Contact Info

Product

Resources

About