Knots Untie: Molecular Determinants Involved in Knot Formation Induced by Pseudomonas savastanoi in Woody Hosts

Caballo-Ponce, Eloy; Murillo, Jesús; Martínez-Gil, Marta; Moreno-Pérez, Alba; Pintado, Adrián; Ramos, Cayo

doi:10.3389/fpls.2017.01089

Cited by 32 publications

(45 citation statements)

References 144 publications

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“…This gene, along with other genes present in the WHOP region, is responsible for the tness and virulence of P. savastanoi pv. savastanoi in woody olive trees, but not in non-woody olives [45,46]. Nitrilotriacetate monooxygenase is known to catabolize plant-derived aromatic compounds and help bacteria to adapt to woody host tissues [47].…”

Section: Discussionmentioning

confidence: 99%

Pan-Genome-Wide Analysis of Pantoea Ananatis Identified Genes Linked to Pathogenicity in Onion

Agarwal¹,

Choudhary²,

Stice³

et al. 2020

Preprint

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Background: Pantoea ananatis is a member of a Pantoea spp. complex that causes center rot of onion, which significantly affects onion yield and quality. This pathogen does not have typical virulence factors like type II or type III secretion systems but appears to require a biosynthetic gene-cluster, HiVir/PASVIL (located chromosomally), for a phosphonate secondary metabolite, and the onion-virulence regions, OVR (localized on a megaplasmid), for onion pathogenicity and virulence, respectively. Results: We conducted a deep pan-genome-wide association study (pan-GWAS) to predict additional genes associated with pathogenicity in P. ananatis using a panel of diverse strains (n = 81). We utilized a red-onion scale necrosis assay as an indicator of pathogenicity. Based on this assay, we differentiated pathogenic (n = 51) - vs. non-pathogenic (n = 30)-strains phenotypically. Pan-GWAS revealed a large core genome of 3,153 genes and a flexible accessory genome of ≤5,065 genes. Phylogenomic analysis using pan-GWAS and presence and absence variants (PAVs) distinguished red-scale necrosis inducing (pathogenic) strains from non-scale necrosis inducing (non-pathogenic) strains of P. ananatis. The pan-GWAS also predicted 42 genes, including 14 from the previously identified HiVir/PASVIL cluster associated with pathogenicity, and 28 novel genes that were not previously associated with pathogenicity in onion. Of the 28 novel genes identified, eight have annotated functions of site-specific tyrosine kinase, N-acetylmuramoyl-L-alanine amidase, TraR/DksA family transcriptional regulator, and HTH-type transcriptional regulator. The remaining 20 genes are currently hypothetical. Conclusions: This is the first report of using pan-GWAS on P. ananatis for the prediction of novel genes contributing to pathogenicity in onion, which will be utilized for further functional analyses. Pan-genomic differences (using PAVs) differentiated onion pathogenic from non-pathogenic strains of P. ananatis, which has been difficult to achieve using single or multiple gene-based phylogenetic analyses. The pan-genome analysis also allowed us to evaluate the presence and absence of HiVir/PASVIL genes and 11 megaplasmid-borne OVR-A genes regarded as the ‘alt’ cluster that aid in P. ananatis colonization in onion bulbs. We concluded that HiVir/PASVIL genes are associated with pathogenic P. ananatis strains and the alt gene cluster alone is not responsible for pathogenicity on onion. The pan-genome also provides clear evidence of constantly evolving accessory genes in P. ananatis that may contribute to host-range expansion and niche-adaptation.

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Section: Discussionmentioning

confidence: 99%

Pan-Genome-Wide Analysis of Pantoea Ananatis Identified Genes Linked to Pathogenicity in Onion

Agarwal¹,

Choudhary²,

Stice³

et al. 2020

Preprint

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“…P. syringae pv. savastanoi NCPPB 3335 causes tumours in olive (Olea europaea) and is a prominent model for the study of the molecular basis of pathogenicity on woody hosts [34,35]. This strain contains three PFP virulence plasmids pPsv48A (80 kb), pPsv48B (45 kb) and pPsv48C (42 kb) [21].…”

Section: Thementioning

confidence: 99%

Integrity and stability of virulence plasmids fromPseudomonas syringaeare modulated by mobile genetic elements and multiple toxin-antitoxin systems

Bardaji

Añorga

Echeverría

et al. 2018

Preprint

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Background: Virulence plasmids are critically exposed to genetic decay and loss, particularly in Pseudomonas syringae strains because of their high content of mobile genetic elements and their exploitation of environmental niches outside of the plant host. The demonstrated high plasticity and adaptability of P. syringae plasmids, involving the acquisition and loss of large DNA regions, contrasts with their usual high stability and the maintenance of key virulence genes in free living conditions. The identification of plasmid stability determinants and mechanisms will help to understand their evolution and adaptability to agroecosystems as well as to develop more efficient control measures. Results:We show that the three virulence plasmids of P. syringae pv. savastanoi NCPPB 3335 contain diverse functional stability determinants, including three toxin-antitoxin systems (TA) in both pPsv48A and pPsv48C, whereas one of the two replicons of pPsv48C can confer stable inheritance by itself. Loss of pPsv48A increased by two orders of magnitude upon functional inactivation of its TA systems. However, inactivation of the TA systems from pPsv48C did not result in its curing but led to the recovery of diverse deletion derivatives. One type consisted in the deletion of an 8.3 kb fragment, with a frequency of 3.8 ± 0.3 × 10 -3 , by recombination between two copies of MITEPsy2. Likewise, IS801 promoted the occurrence of deletions of variable size by one-ended transposition with a frequency of 5.5 ± 2.1 × 10 -4 , 80 % of which resulted in the loss of virulence gene idi. These deletion derivatives were stably maintained in the population by replication mediated by repJ, which is adjacent to IS801. IS801 also promoted deletions in plasmid pPsv48A, either by recombination or one-ended transposition. In all cases, functional TA systems contributed significantly to reduce the occurrence of plasmid deletions in vivo. Conclusions:Virulence plasmids from P. syringae harbour a diverse array of stability determinants with a variable contribution to plasmid persistence. Additionally, multiple TA systems favour the long-term survival and integrity of virulence plasmids, as well as the maintenance of pathogenicity genes in free-living conditions. This strategy is likely widespread amongst native plasmids of P. syringae and other bacteria.(MINECO), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER).

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“…The species P. savastanoi , which induces knots or excrescences in woody plants, is divided into four pathovars, named for the plant from which they were isolated, for example P. savastanoi pv. savastanoi ( Olea europaea ) …”

Section: Introductionmentioning

confidence: 99%

Correlation between VOC fingerprinting and antimicrobial activity of several essential oils extracted by plant resins against A. tumefaciens and P. savastanoi

Caparrotta

Comparini

Marone

et al. 2019

Flavour & Fragrance J

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The present work aimed to assess the antimicrobial activity of twelve essential oils extracted from plant resins of trees that grow in several tropical and subtropical regions of the world. Eleven essential oils were extracted from resins of plant belonging to the Burseraceae family (the genera Boswellia, Commiphora, Canarium and Bursera); the remaining oil was extracted from a resinous plant belonging to the Fabaceae family (genus Hymenea). The extraction was carried out using a conventional distillation method. The volatile organic compounds (VOCs) of each essential oil were analyzed with PTR‐ToF‐MS (proton transfer reaction time‐of‐flight mass spectrometry) and the antimicrobial activity was evaluated against the phytopathogens Agrobacterium tumefaciens and Pseudomonas savastanoi pv. savastanoi. The essential oils obtained from Boswellia papyrifera, B. dalzielii, B. frereana and B. rivae were the most abundant in terms of signal intensity and VOC number in comparison with the other analyzed essential oils. Furthermore, B. neglecta shared a common volatile profile with the Canarium species, as was demonstrated previously in the literature. Greater bacterial growth inhibition of A. tumefaciens was detected with the essential oils extracted from plants belonging to Boswellia species while less action was observed against P. savastanoi. Finally, a positive correlation was found between terpenes and terpenoid compounds and bacterial growth inhibition, suggesting these essential oils as a new source of bioactive compounds for the prevention, protection and treatment of plant diseases.

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Knots Untie: Molecular Determinants Involved in Knot Formation Induced by Pseudomonas savastanoi in Woody Hosts

Cited by 32 publications

References 144 publications

Pan-Genome-Wide Analysis of Pantoea Ananatis Identified Genes Linked to Pathogenicity in Onion

Pan-Genome-Wide Analysis of Pantoea Ananatis Identified Genes Linked to Pathogenicity in Onion

Integrity and stability of virulence plasmids fromPseudomonas syringaeare modulated by mobile genetic elements and multiple toxin-antitoxin systems

Correlation between VOC fingerprinting and antimicrobial activity of several essential oils extracted by plant resins against A. tumefaciens and P. savastanoi

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