Knockout of miR-21-5p alleviates cartilage matrix degradation by targeting Gdf5 in temporomandibular joint osteoarthritis

Zhang, Aobo; Ma, Shixing; Yuan, Linlin; Wu, Shichao; Liu, Shaopeng; Xiang, Wei; Chen, Lei; Ma, Chuan; Zhao, Hongxia

doi:10.1302/2046-3758.910.bjr-2020-0140.r1

Cited by 19 publications

(17 citation statements)

References 38 publications

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“…80 Researchers suggested that both miR‐101a‐3p and miR21-5p was involved in the cartilage matrix degradation as well as in progression of degenerative changes of TMJ. 81 …”

Section: Role Of Mirnas In Tmd Painmentioning

confidence: 99%

“… Sato et al 71 Growth factors NGF and VEGF Plasma and Synovial Fluid Persistent pain and pain progression in TMD disc and degenerative joint diseases. Chen et al 74 , Ke et al 75 Epigenetic biomarkers (miRNA) Tissue: Synovial fibroblasts and articular cartilage Altered expression of miRNA221–3p, miRNA 140-5p, miR‐101a‐3p, miR21-5p were observed in DJD and Pain Zhang et al 81 , Li et al 79 , Xu et al 78 Neuroimaging biomarkers Structural MRI Decreased trigeminal nerve fiber density, axonal diameter, myelination and GMV correlated well with TMD pain in patients with TMJ synovitis and myofascial pain. Zhang et al 91 , Wilcox et al 87 , He et al 98 , Moayedi et al 88 , Gustin et al 89 , Weissman-Fogel et al 94 , Ichesco et al 92 , Younger et al 90 , Buckner et al 93 Functional MRI Alterations in FC, GMV CBF, MD was observed in different regions of high order cognition, emotion-related regions such as ACC, PCC, MCCC, mPFC, DLPFC, Amygdala, and PAG-raphe system.…”

Section: Introductionmentioning

confidence: 99%

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A comprehensive review on biomarkers associated with painful temporomandibular disorders

Shrivastava

Battaglino

2021

Int J Oral Sci

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Pain of the orofacial region is the primary complaint for which patients seek treatment. Of all the orofacial pain conditions, one condition that possess a significant global health problem is temporomandibular disorder (TMD). Patients with TMD typically frequently complaints of pain as a symptom. TMD can occur due to complex interplay between peripheral and central sensitization, endogenous modulatory pathways, and cortical processing. For diagnosis of TMD pain a descriptive history, clinical assessment, and imaging is needed. However, due to the complex nature of pain an additional step is needed to render a definitive TMD diagnosis. In this review we explicate the role of different biomarkers involved in painful TMD. In painful TMD conditions, the role of biomarkers is still elusive. We believe that the identification of biomarkers associated with painful TMD may stimulate researchers and clinician to understand the mechanism underlying the pathogenesis of TMD and help them in developing newer methods for the diagnosis and management of TMD. Therefore, to understand the potential relationship of biomarkers, and painful TMD we categorize the biomarkers as molecular biomarkers, neuroimaging biomarkers and sensory biomarkers. In addition, we will briefly discuss pain genetics and the role of potential microRNA (miRNA) involved in TMD pain.

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“…80 Researchers suggested that both miR‐101a‐3p and miR21-5p was involved in the cartilage matrix degradation as well as in progression of degenerative changes of TMJ. 81 …”

Section: Role Of Mirnas In Tmd Painmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A comprehensive review on biomarkers associated with painful temporomandibular disorders

Shrivastava

Battaglino

2021

Int J Oral Sci

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“…32,33 miRNAs have been shown to be closely related to the progression and severity of OA. 34,35 Therefore, the regulatory mechanism underlying circSCAPER in OA was further explored, and we confirmed that circSCAPER directly binds to miR-140-3p. miR-140-3p isomiRs are abundantly expressed in cartilage, which is more effective than the original miR-140-3p annotated in miRBase [36][37][38] in human and murine chondrocytes, 39 suggesting the potential function of miR-140-3p in OA.…”

Section: Discussionmentioning

confidence: 60%

CircSCAPER contributes to IL-1β-induced osteoarthritis in vitro via miR-140-3p/EZH2 axis

Luobu

Wang

Jiang

et al. 2022

Bone & Joint Research

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Aims Circular RNA (circRNA) S-phase cyclin A-associated protein in the endoplasmic reticulum (ER) (circSCAPER, ID: hsa_circ_0104595) has been found to be highly expressed in osteoarthritis (OA) patients and has been associated with the severity of OA. Hence, the role and mechanisms underlying circSCAPER in OA were investigated in this study. Methods In vitro cultured human normal chondrocyte C28/I2 was exposed to interleukin (IL)-1β to mimic the microenvironment of OA. The expression of circSCAPER, microRNA (miR)-140-3p, and enhancer of zeste homolog 2 (EZH2) was detected using quantitative real-time polymerase chain reaction and Western blot assays. The extracellular matrix (ECM) degradation, proliferation, and apoptosis of chondrocytes were determined using Western blot, cell counting kit-8, and flow cytometry assays. Targeted relationships were predicted by bioinformatic analysis and verified using dual-luciferase reporter and RNA immunoprecipitation (RIP) assays. The levels of phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway-related protein were detected using Western blot assays. Results CircSCAPER was highly expressed in OA cartilage tissues and IL-1β-induced chondrocytes. Knockdown of circSCAPER reduced IL-1β-evoked ECM degradation, proliferation arrest, and apoptosis enhancement in chondrocytes. Mechanistically, circSCAPER directly bound to miR-140-3p, and miR-140-3p inhibition reversed the effects of circSCAPER knockdown on IL-1β-induced chondrocytes. miR-140-3p was verified to target EZH2, and overexpression of miR-140-3p protected chondrocytes against IL-1β-induced dysfunction via targeting EZH2. Additionally, we confirmed that circSCAPER could regulate EZH2 through sponging miR-140-3p, and the circSCAPER/miR-140-3p/EZH2 axis could activate the PI3K/AKT pathway. Conclusion CircSCAPER promoted IL-1β-evoked ECM degradation, proliferation arrest, and apoptosis enhancement in chondrocytes via regulating miR-140-3p/EZH2 axis, which gained a new insight into the pathogenesis of OA. Cite this article: Bone Joint Res 2022;11(2):61–72.

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“…MiR-21 inhibits the expression of GDF-5 and its overexpression attenuates the progression of OA ( Zhang et al, 2014 ). This finding was validated in the mouse temporomandibular joint osteoarthritis (TMJOA) model, where Zhang et al claimed that knockdown of miR-21-5p reduced cartilage matrix degradation in TMJOA by targeting GDF-5 ( Zhang A. et al, 2020 ). Also in the mouse TMJOA model, researchers discovered that miR-21-5p knockout mice had less temporomandibular joint cartilage destruction than wild-type mice, and in vitro experiments showed that miR-21-5p promotes extracellular matrix degradation and angiogenesis in TMJOA by suppressing the expression of target gene SPRY1, which in turn promotes the development of TMJOA ( Ma et al, 2020 ).…”

Section: The Regulatory Role Of Mir-21 In Oamentioning

confidence: 80%

MicroRNA-21: An Emerging Player in Bone Diseases

Chen

et al. 2021

Front. Pharmacol.

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MicroRNAs (MiRNAs) are small endogenous non-coding RNAs that bind to the 3′-untranslated region of target genes and promote their degradation or inhibit translation, thereby regulating gene expression. MiRNAs are ubiquitous in biology and are involved in many biological processes, playing an important role in a variety of physiological and pathological processes. MiRNA-21 (miR-21) is one of them. In recent years, miR-21 has received a lot of attention from researchers as an emerging player in orthopedic diseases. MiR-21 is closely associated with the occurrence, development, treatment, and prevention of orthopedic diseases through a variety of mechanisms. This review summarizes its effects on osteoblasts, osteoclasts and their relationship with osteoporosis, fracture, osteoarthritis (OA), osteonecrosis, providing a new way of thinking for the diagnosis, treatment and prevention of these bone diseases.

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Knockout of miR-21-5p alleviates cartilage matrix degradation by targeting Gdf5 in temporomandibular joint osteoarthritis

Cited by 19 publications

References 38 publications

A comprehensive review on biomarkers associated with painful temporomandibular disorders

A comprehensive review on biomarkers associated with painful temporomandibular disorders

CircSCAPER contributes to IL-1β-induced osteoarthritis in vitro via miR-140-3p/EZH2 axis

MicroRNA-21: An Emerging Player in Bone Diseases

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