CircSCAPER contributes to IL-1β-induced osteoarthritis in vitro via miR-140-3p/EZH2 axis

Luobu, Zhaxi; Wang, Lei; Jiang, Dahai; Liao, Tao; Luobu, Ciren; Qunpei, Luosong

doi:10.1302/2046-3758.112.bjr-2020-0482.r2

Cited by 17 publications

(12 citation statements)

References 48 publications

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“…This upregulation aggravates IL-1β-induced injury by downregulating IL-1β and reducing miR-142-5p in IL-1β-stimulated chondrocytes [ 138 ]. CircSCAPER promotes IL-1β-induced ECM degradation, proliferation arrest, and apoptosis enhancement in human chondrocytes by regulating the miR-140-3p/EZH2 axis [ 139 ]. The circRNA derived from vacuolar ATPase assembly factor ( VMA21 ) suppresses LPS-induced chondrocyte apoptosis in OA by decreasing the production of mature miR-103 [ 140 ].…”

Section: Ncrnas Involved In Cartilage Homeostasis and Oamentioning

confidence: 99%

Cartilage Homeostasis and Osteoarthritis

Fujii

Liu

Yagasaki

et al. 2022

IJMS

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Healthy limb joints are important for maintaining health and attaining longevity. Endochondral ossification (the replacement of cartilage with bone, occurring during skeletal development) is essential for bone formation, especially in long-axis bones. In contrast to endochondral ossification, chondrocyte populations in articular cartilage persist and maintain joint tissue into adulthood. Articular cartilage, a connective tissue consisting of chondrocytes and their surrounding extracellular matrices, plays an essential role in the mechanical cushioning of joints in postnatal locomotion. Osteoarthritis (OA) pathology relates to disruptions in the balance between anabolic and catabolic signals, that is, the loss of chondrocyte homeostasis due to aging or overuse of cartilages. The onset of OA increases with age, shortening a person’s healthy life expectancy. Although many people with OA experience pain, the mainstay of treatment is symptomatic therapy, and no fundamental treatment has yet been established. To establish regenerative or preventative therapies for cartilage diseases, further understanding of the mechanisms of cartilage development, morphosis, and homeostasis is required. In this review, we describe the general development of cartilage and OA pathology, followed by a discussion on anabolic and catabolic signals in cartilage homeostasis, mainly microRNAs.

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Section: Ncrnas Involved In Cartilage Homeostasis and Oamentioning

confidence: 99%

Cartilage Homeostasis and Osteoarthritis

Fujii

Liu

Yagasaki

et al. 2022

IJMS

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“…Hence, hsa_circ_0027914 might down-regulate miR-140-3p through a competitive endogenous mechanism and lead to upregulation of DEGs in OA. A recent study has also reported that another circRNA (i.e., hsa_circ_0104595) can sponge miR-140-3p, then target EZH2 (enhancer of zeste homolog 2) to induce apoptosis of chondrocytes in OA ( 44 ). Furthermore, when the effect of si-circ-0027914 in reducing apoptosis is taken into consideration, there are reasons to believe that hsa_circ_0027914 plays a vital role in regulating multiple miRNAs and the progression of OA.…”

Section: Discussionmentioning

confidence: 99%

Identification and comprehensive analysis of circRNA–miRNA–mRNA regulatory networks in osteoarthritis

et al. 2023

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Osteoarthritis (OA) is a common orthopedic degenerative disease, leading to high disability in activities of daily living. There remains an urgent need to identify the underlying mechanisms and identify new therapeutic targets in OA diagnosis and treatment. Circular RNAs (circRNAs) play a role in the development of multiple diseases. Many studies have reported that circRNAs regulate microRNAs (miRNAs) through an endogenous competitive mechanism. However, it remains unclear if an interplay between circRNAs, miRNAs, and target genes plays a deeper regulatory role in OA. Four datasets were downloaded from the GEO database, and differentially expressed circRNAs (DECs), differentially expressed miRNAs (DEMs), and differentially expressed genes (DEGs) were identified. Functional annotation and pathway enrichment analysis of DEGs and DECs were carried out to determine the main associated mechanism in OA. A protein–protein network (PPI) was constructed to analyze the function of, and to screen out, hub DEGs in OA. Based on the artificial intelligence prediction of protein crystal structures of two hub DEGs, TOP2A and PLK1, digitoxin and oxytetracycline were found to have the strongest affinity, respectively, with molecular docking. Subsequently, overlapping DEMs and miRNAs targeted by DECs obtained target DEMs (DETMs). Intersection of DEGs and genes targeted by DEMs obtained target DEGs (DETGs). Thus, a circRNA–miRNA–mRNA regulatory network was constructed from 16 circRNAs, 32 miRNAs, and 97 mRNAs. Three hub DECs have the largest number of regulated miRNAs and were verified through in vitro experiments. In addition, the expression level of 16 DECs was validated by RT-PCR. In conclusion, we constructed a circRNA–miRNA–mRNA regulatory network in OA and three new hub DECs, hsa_circ_0027914, hsa_circ_0101125, and hsa_circ_0102564, were identified as novel biomarkers for OA.

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“…All individuals participating were informed of the study and signed informed consent. e protocol of this work was approved by the Ethics Committee of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology [16].…”

Section: Methodsmentioning

confidence: 99%

Circular RNA MELK Promotes Chondrocyte Apoptosis and Inhibits Autophagy in Osteoarthritis by Regulating MYD88/NF‐κB Signaling Axis through MicroRNA‐497‐5p

Zhang

Huang

et al. 2022

Contrast Media & Molecular Imaging

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Osteoarthritis (OA) is a rheumatic disease and its pathogenesis involves the dysregulation of noncoding RNAs. Therefore, the regulatory mechanism of circular RNA MELK (circMELK) was specified in this work. OA human cartilage tissue was collected, and circMELK, miR-497-5p, and myeloid differentiation factor 88 (MYD88) expression were examined. Human chondrocytes were stimulated with interleukin- (IL-) 1β and interfered with vectors altering circMELK, miR-497-5p, and MyD88 expression to observe their effects on cell viability, cell cycle and apoptosis, autophagy, and inflammation. The binding relationship between RNAs was verified. The data presented that OA cartilage tissues presented raised circMELK and MYD88 and inhibited miR-497-5p expression. IL-1β suppressed cell viability, prevented cell cycle, and induced apoptosis, autophagy, and inflammation of chondrocytes. Functionally, IL-1β-induced changes of chondrocytes could be attenuated by suppressing circMELK or overexpressing miR-497-5p. circMELK acted as a sponge of miR-497-5p while miR-497-5p was a regulator of MYD88. MYD88 restricted the effect of overexpressing miR-497-5p on IL-1β-stimulated chondrocytes. MYD88 triggered nuclear factor-kappaB (NF-κB) pathway activation. Shortly, CircMELK promotes chondrocyte apoptosis and inhibits autophagy in OA by regulating MYD88/NF-κB signaling axis through miR-497-5p. Our study proposes a new molecular mechanism for the development of OA.

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CircSCAPER contributes to IL-1β-induced osteoarthritis in vitro via miR-140-3p/EZH2 axis

Cited by 17 publications

References 48 publications

Cartilage Homeostasis and Osteoarthritis

Cartilage Homeostasis and Osteoarthritis

Identification and comprehensive analysis of circRNA–miRNA–mRNA regulatory networks in osteoarthritis

Circular RNA MELK Promotes Chondrocyte Apoptosis and Inhibits Autophagy in Osteoarthritis by Regulating MYD88/NF‐κB Signaling Axis through MicroRNA‐497‐5p

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