Knockdown of TSPAN1 by RNA Silencing and Antisense Technique Inhibits Proliferation and Infiltration of Human Skin Squamous Carcinoma Cells

Abstract

The TSPAN1 gene might play a role in the proliferation of squamous cell carcinoma of the skin and be associated with cancer cell motility, implying a function of the gene in the development of skin cancer.

Tetraspanin 1 is involved in survival, proliferation and carcinogenesis of pancreatic cancer

“…Tspan1, a new member of the tetraspanins group, was found to be elevated in some tumors (12,(23)(24)(25). Recent studies also suggested Tspan1 gene may play a role in the proliferation of cancer cells and be associated with cancer cell motility, implying a function of the gene in the development of various cancer (12,23,26). In this study, our results revealed that Tspan1 immunopositive staining was significantly correlated with the lymph node metastasis, pTNM stages and poor prognosis of PCC.…”

“…The molecule may accept extracellular signals when located on the membrane and carry out functions in the cytoplasm, like other tetraspanins such as CD9, CD82 and CD63 (12,17,18). In the present study, we found that the expression of Tspan1 in PCC tissues was significantly higher than the normal adjacent tissues.…”

“…our results revealed that expression of Tspan1 in PCC cells displayed cytoplasmic patterns, which showed the distribution and functional sites of the Tspan1 molecule in cells (12). The molecule may accept extracellular signals when located on the membrane and carry out functions in the cytoplasm, like other tetraspanins such as CD9, CD82 and CD63 (12,17,18).…”

“…In many human cancers, tumor progression was found to be associated with an altered expression of tetraspanins (22). Tspan1, a new member of the tetraspanins group, was found to be elevated in some tumors (12,(23)(24)(25). Recent studies also suggested Tspan1 gene may play a role in the proliferation of cancer cells and be associated with cancer cell motility, implying a function of the gene in the development of various cancer (12,23,26).…”

“…TEMs of various types are reportedly involved in the regulation of cell growth, migration and invasion of several tumor cell types, both as suppressors or supporting structures (8). Tetraspanin 1 (Tspan1), a member of the transmembrane 4 superfamily of tetraspanins, is overexpressed in high-grade cervical intraepithelial neoplasia (CIN) and terminal carcinomas (8), lung cancer (9), colon cancer (10), breast cancer (11), as well as squamous cell carcinoma (12). However, the precise function of Tspan1 in the context of PCC is not known.…”