Knockdown of Nemo‑like kinase promotes metastasis in non‑small‑cell lung cancer

Shi, Cui; Xu, Liqin; Tang, Zhiyuan; Zhang, Weishuai; Wei, Yu-Lin; Ni, Jun; Zhang, Shuwen; Feng, Jian

doi:10.3892/or.2019.7226

Cited by 5 publications

(4 citation statements)

References 49 publications

(45 reference statements)

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“…They control several transcription factors, take part in a number of signaling pathways, including Notch, MAPK, and Wnt, etc. (Zhang et al, 2015;Shi et al, 2019). In this study, the expression levels of these genes in the visceral mass tissue after nucleation were mostly down-regulated compared with the mantle.…”

Section: Discussionmentioning

confidence: 45%

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Shen,

Chen,

Jia

et al. 2023

Front. Mar. Sci.

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Biomineralization is a widespread phenomenon in marine mollusks and is responsible for the production of shells and pearls. However, the regulatory mechanisms governing the adaptive immune responses in the mollusk mantle and visceral mass during mineralization remain unclear. In this work, we examined the mantle and visceral mass immune responses of Hyriopsis cumingii during pearl culture using high-throughput sequencing techniques. A mantle transcriptome database was established using transcriptome sequencing technology and reference to the major databases. Digital gene expression profiling was used to identify the differentially expressed genes of mantle and visceral mass at different insertion periods. Moreover, quantitative real-time PCR was used to verify the expression of five immune-related genes. Transcriptome sequencing results showed 257,457 unigenes were identified. Digital gene expression profiles showed 1389, 3572, 1888, and 2613 differentially expressed genes (DEGs) in the mantle and visceral mass at 5, 20, 50, and 90 d after insertion, respectively, with the highest number at 20 d and the lowest at 5 d after insertion (q < 0.05). A cluster analysis of the DEGs showed similar clustering and expression features in the mantle to the control group, and at 5, 50 and 90 d, after mantle insertion. The DEGs in the visceral mass showed similar clustering and expression features to the control group and at 5, 20 and 50 d after insertion. We also screened 22 immune-related DEGs in the mantle and visceral mass during the same pearl culture period, including serine/threonine-protein kinase NLK, C-type lectin, and galectin. The greatest number of DEGs was found 90 d after insertion. Compared with the mantle, more immune-related DEGs were down-regulated than up-regulated in the visceral mass during pearl culture, indicating that the immune regulatory mechanisms in the visceral mass and the mantle differ during pearl culture, and that the visceral mass is liable to higher infection and mortality rates. Quantitative real-time PCR results showed that the expression of five immune-related genes was consistent with DGE results. Our findings will further knowledge of the immune systems that are present in the mantle and visceral mass during pearl culture.

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Section: Discussionmentioning

confidence: 45%

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Shen,

Chen,

Jia

et al. 2023

Front. Mar. Sci.

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“…In contrast, it was found to negatively regulate the ternary transcriptional complex in the Notch signaling pathway that is crucial in cell fates of metazoan tissues [ 65 ]. Aberrant expression of NLK has been associated with the initiation or progression of oral squamous cell carcinoma [ 66 ], laryngeal cancer [ 67 ], non-small-cell lung cancer (NSCLC) [ 68 , 69 ], and CRC [ 70 ]. The CAMKK1 gene mediated the upstream activation of AMP-activated protein kinase (AMPK) by phosphorylation of the α-subunit threonine 172 (Thr172) residue.…”

Section: Discussionmentioning

confidence: 99%

Identification of Differentially Methylated CpG Sites in Fibroblasts from Keloid Scars

et al. 2020

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As a part of an abnormal healing process of dermal injuries and irritation, keloid scars arise on the skin as benign fibroproliferative tumors. Although the etiology of keloid scarring remains unsettled, considerable recent evidence suggested that keloidogenesis may be driven by epigenetic changes, particularly, DNA methylation. Therefore, genome-wide scanning of methylated cytosine-phosphoguanine (CpG) sites in extracted DNA from 12 keloid scar fibroblasts (KF) and 12 control skin fibroblasts (CF) (six normal skin fibroblasts and six normotrophic fibroblasts) was conducted using the Illumina Human Methylation 450K BeadChip in two replicates for each sample. Comparing KF and CF used a Linear Models for Microarray Data (Limma) model revealed 100,000 differentially methylated (DM) CpG sites, 20,695 of which were found to be hypomethylated and 79,305 were hypermethylated. The top DM CpG sites were associated with TNKS2, FAM45B, LOC723972, GAS7, RHBDD2 and CAMKK1. Subsequently, the most functionally enriched genes with the top 100 DM CpG sites were significantly (p ≤ 0.05) associated with SH2 domain binding, regulation of transcription, DNA-templated, nucleus, positive regulation of protein targeting to mitochondrion, nucleoplasm, Swr1 complex, histone exchange, and cellular response to organic substance. In addition, NLK, CAMKK1, LPAR2, CASP1, and NHS showed to be the most common regulators in the signaling network analysis. Taken together, these findings shed light on the methylation status of keloids that could be implicated in the underlying mechanism of keloid scars formation and remission.

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“…NLK has been found to regulate multiple signaling pathways and participate in various biological processes [17][18][19] . Abnormal expression of NLK is closely associated with the occurrence and progression of tumors, with high expression in laryngeal cancer, lung cancer, and cervical squamous cell carcinoma [20][21][22] , but with low expression in non-small cell lung cancer, breast cancer, and ovarian cancer [23][24][25] . Currently, there are no reports on the expression of NLK in pancreatic cancer and its relationship with prognosis.…”

Section: Introductionmentioning

confidence: 99%

MSI2 regulates NLK-mediated EMT and PI3K/AKT/mTOR pathway to promote pancreatic cancer progression

Huang,

Sun,

et al. 2024

Preprint

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Background: The incidence of pancreatic cancer is increasing by years, and the 5-year survival rate is very low. Our team have revealed that Musashi2 (MSI2) could promote aggressive behaviors in pancreatic cancer by downregulating Numb and p53. MSI2 also facilitates EMT in pancreatic cancer induced by EGF through the ZEB1-ERK/MAPK signaling pathway. This study aims to further explore the molecular mechanisms of MSI2-regulated downstream pathways in pancreatic cancer. Methods: In vitro and in vivo experiments were conducted to investigate the role and mechanism of MSI2 in promoting malignant behaviors of pancreatic cancer through regulation of NLK. Results: Genes closely related to MSI2 were screened from the GEPIA and TCGA databases. We found that NLK showed the most significant changes in mRNA levels with consistent changes following MSI2 interference and overexpression. The high correlation between MSI2 and NLK was also observed at the protein level. Multivariate analysis revealed that both MSI2 and NLK were independent adverse indicators of survival in pancreatic cancer patients, as well as join together. In vitro, silencing or overexpressing NLK altered cell invasion and migration, by regulating EMT and the PI3K-AKT-mTOR pathway. Silencing MSI2 reduced protein expression in the EMT and PI3K-AKT-mTOR pathways, leading to decreased cell invasion and migration abilities, while these effects could be reversed by overexpression of NLK. In vivo, MSI2 silencing inhibited liver metastasis, which could be reversed by overexpressing NLK. Conclusions: Our findings elucidate the regulatory mechanisms of the MSI2-NLK axis in modulating aggressive behaviors of pancreatic cancer cells, which providing new evidence for therapeutic strategies in pancreatic cancer.

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Knockdown of Nemo‑like kinase promotes metastasis in non‑small‑cell lung cancer

Cited by 5 publications

References 49 publications

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Identification of Differentially Methylated CpG Sites in Fibroblasts from Keloid Scars

MSI2 regulates NLK-mediated EMT and PI3K/AKT/mTOR pathway to promote pancreatic cancer progression

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