Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma

Abstract

Background: LncRNAs play crucial roles in the development of carcinomas. However, the investigation of LINC00662 in Oral squamous cell carcinoma (OSCC) is still elusive. Methods: qRT-PCR assay tested the expression levels of LINC00662, hnRNPC and AK4. With exposure to irradiation, CCK-8, colony formation, flow cytometry and western blot experiments, respectively determined the function of LINC00662 in the radiosensitivity of OSCC cells. Then RIP and western blot assays affirmed the interaction between hnRNPC protein and LINC00662 or AK4. Finally, rescue assays validated the regulation mechanism of LINC00662 in the radioresistance of OSCC. Results: In the present report, LINC00662 was overexpressed in OSCC and its silencing could alleviate radioresistance of OSCC. Furthermore, the interaction between hnRNPC protein and LINC00662 or AK4 was uncovered. Besides, LINC00662 regulated AK4 mRNA stability through binding to hnRNPC protein. To sum up, LINC00662 modulated the radiosensitivity of OSCC cells via hnRNPC-modulated AK4. Conclusion: The molecular mechanism of the LINC00662/hnRNPC/AK4 axis was elucidated in OSCC, which exhibited a promising therapeutic direction for patients with OSCC.

The Vital Roles of LINC00662 in Human Cancers

“…LINC00662/hnRNPC/mRNA for AK4 Chen et al (2020) reported that LINC00662 could interact with both heterogeneous nuclear ribonucleoprotein C (hnRNPC) and adenylate kinase 4 (AK4) in OSCC and that LINC00662 mainly interacted with hnRNPC protein to regulate AK4 mRNA stability, resulting in AK4 protein overexpression. Interestingly, LINC00662 was shown not to be able to interact with hnRNPC directly, and its modulation of OSCC cell radiosensitivity was shown to be through hnRNPC-modulated AK4.…”

“…Among head and neck cancers, oral squamous cell carcinoma (OSCC) is considered highly aggressive and represents the top cause of deaths globally ( Xu et al, 2019 ; Chen et al, 2020 ). Notably, Chen et al (2020) revealed that compared to normal tissues, LINC00662 expression was elevated in OSCC and that blocking its expression ameliorated OSCC radio-resistance.…”

“…Among head and neck cancers, oral squamous cell carcinoma (OSCC) is considered highly aggressive and represents the top cause of deaths globally ( Xu et al, 2019 ; Chen et al, 2020 ). Notably, Chen et al (2020) revealed that compared to normal tissues, LINC00662 expression was elevated in OSCC and that blocking its expression ameliorated OSCC radio-resistance. Similarly, Xu et al (2019) also demonstrated that the overexpression of LINC00662 was closely correlated with the adverse clinical characteristics of tumor size, tumor stage, and lymph node metastasis ascribed to its influences on OSCC cell proliferation and motility.…”

“…-5p/HOXB2 axis esophageal squamous cell carcinomaZhang et al (2020) miR-497-5p/YAP1 axis gastric cancerLiu et al (2018) hnRNPC/AK4 axis oral squamous cell carcinomaChen et al (2020) miR-890/ELK3 axis melanomaXia et al (2020) miR-340-5p/ROCK1 axis acute myeloid leukemiaLiu Y. et al (2019) …”

Suppression of Heterogeneous Nuclear Ribonucleoprotein C Inhibit Hepatocellular Carcinoma Proliferation, Migration, and Invasion via Ras/MAPK Signaling Pathway

“…A known RNA-binding protein, heterogeneous nuclear ribonucleoprotein C (HNRNPC) plays an important part in RNA splicing (24,25), stability (26,27), and expression (28). HNRNPC has been identified as an oncogene, enhancing deterioration in multiple tumor types, including gastric cancer (29), breast cancer (27), esophageal squamous cell carcinoma (26), and oral squamous cell carcinoma (30). According to previous reports, HNRNPC primarily regulates the biological activity of the IFNb signaling pathway (27), p53 gene (31), and AKT signaling pathway (32), which are associated with tumor proliferation, invasion, and metastasis.…”