Knockdown of linc-POU3F3 suppresses the proliferation, apoptosis, and migration resistance of colorectal cancer

Shan, Ti‐Dong; Xu, Jihao; Yu, Tao; Li, Jieyao; Zhao, Linna; Ouyang, Hui; Luo, Su; Lu, Xi‐Ji; Huang, Cuiqing; Lan, Qiu-Shen; Wu, Zhong; Chen, Qi‐Kui

doi:10.18632/oncotarget.5830

Cited by 51 publications

(45 citation statements)

References 50 publications

(48 reference statements)

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“…In the present study, our data showed that ZEB1-AS1 was over-expressed in CRC tissues and positively correlated with tumor histology grade, N grade, and M grade. These results suggested that ZEB1-AS1 might be a potential diagnostic biomarker or therapeutic target in CRC [19][20][21]. To further understood the role of ZEB1-AS1 in CRC, we chose to detect changes in the biological behaviors in CRC cell lines.…”

Section: Discussionmentioning

confidence: 99%

The lncRNA ZEB1-AS1 sponges miR-181a-5p to promote colorectal cancer cell proliferation by regulating Wnt/β-catenin signaling

Shan

Pan

et al. 2018

Cell Cycle

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Long noncoding RNAs (lncRNAs) are important regulators of the biological functions and underlying molecular mechanisms of colorectal cancer (CRC). However, the role of the lncRNA ZEB1-AS1 in CRC is not thoroughly understood. In this study, we found that ZEB1-AS1 was markedly upregulated in CRC. ZEB1-AS1 knockdown significantly suppressed CRC cell proliferation and induced apoptosis, whereas enhanced expression of ZEB1-AS1 had the opposite effect. Bioinformatics analysis identified miR-181a-5p as a candidate target of ZEB1-AS1. Moreover, we found an inverse correlation between ZEB1-AS1 and miR-181a-5p expression in CRC tissue. Inhibition of miR-181a-5p significantly upregulated ZEB1-AS1, whereas overexpression of miR-181a-5p had the opposite effect, suggesting that ZEB1-AS1 is negatively regulated by miR-181a-5p. Using luciferase reporter and RIP assays, we found that miR-181a-5p directly targets ZEB1-AS1. Importantly, ZEB1-AS1 may act as an endogenous 'sponge' to regulate miRNA targets by competing for miR-181a-5p binding. In summary, our findings provide the evidence supporting the role of ZEB1-AS1 as an oncogene in CRC. Our study also demonstrates that miR-181a-5p targets not only protein-coding genes but also the lncRNA ZEB1-AS1.

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Section: Discussionmentioning

confidence: 99%

The lncRNA ZEB1-AS1 sponges miR-181a-5p to promote colorectal cancer cell proliferation by regulating Wnt/β-catenin signaling

Shan

Pan

et al. 2018

Cell Cycle

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“…Long non-coding RNAs (lncRNAs) are a class of non-protein coding RNAs of more than 200 nucleotides, which are broadly distributed in the genome and can modulate gene expression [19–22]. Recent accumulating evidence has demonstrated that lncRNA expression profiles are frequently changed in tumors compared to that of the adjacent non-tumorous tissues in numerous cancers [23–27].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive investigation of a novel differentially expressed lncRNA expression profile signature to assess the survival of patients with colorectal adenocarcinoma

Zeng

Liang

et al. 2017

Oncotarget

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Growing evidence has shown that long non-coding RNAs (lncRNAs) can serve as prospective markers for survival in patients with colorectal adenocarcinoma. However, most studies have explored a limited number of lncRNAs in a small number of cases. The objective of this study is to identify a panel of lncRNA signature that could evaluate the prognosis in colorectal adenocarcinoma based on the data from The Cancer Genome Atlas (TCGA). Altogether, 371 colon adenocarcinoma (COAD) patients with complete clinical data were included in our study as the test cohort. A total of 578 differentially expressed lncRNAs (DELs) were observed, among which 20 lncRNAs closely related to overall survival (OS) in COAD patients were identified using a Cox proportional regression model. A risk score formula was developed to assess the prognostic value of the lncRNA signature in COAD with four lncRNAs (LINC01555, RP11-610P16.1, RP11-108K3.1 and LINC01207), which were identified to possess the most remarkable correlation with OS in COAD patients. COAD patients with a high-risk score had poorer OS than those with a low-risk score. The multivariate Cox regression analyses confirmed that the four-lncRNA signature could function as an independent prognostic indicator for COAD patients, which was largely mirrored in the validating cohort with rectal adenocarcinoma (READ) containing 158 cases. In addition, the correlative genes of LINC01555 and LINC01207 were enriched in the cAMP signaling and mucin type O-Glycan biosynthesis pathways. With further validation in the future, our study indicates that the four-lncRNA signature could serve as an independent biomarker for survival of colorectal adenocarcinoma.

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“…As previously mentioned, linc-POU3F3 was an oncogene that silencing linc-POU3F3 inhibited proliferation and migration, induced apoptosis in CRC. SMAD4, which is involved in TGF-β-induced autophagy, showed an increase because of linc-POU3F3 knockdown [52, 53]. In addition, autophagy related protein, BECLIN1, ATG5, ATG7, and LC3 II, were continually accumulated by linc-POU3F3 knockdown due to interdicted autophagy in CRC [53].…”

Section: The Role Of Lncrnas In Crcmentioning

confidence: 99%

“…SMAD4, which is involved in TGF-β-induced autophagy, showed an increase because of linc-POU3F3 knockdown [52, 53]. In addition, autophagy related protein, BECLIN1, ATG5, ATG7, and LC3 II, were continually accumulated by linc-POU3F3 knockdown due to interdicted autophagy in CRC [53]. This study declared that linc-POU3F3 exerts its functional potential by mediating CRC autophagy.…”

Section: The Role Of Lncrnas In Crcmentioning

confidence: 99%

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The Biological Effect and Clinical Application of Long Noncoding RNAs in Colorectal Cancer

Sun¹,

Wang²,

Chen³

et al. 2018

Cell Physiol Biochem

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Colorectal cancer (CRC) is one of the most common malignancies in the world. Easier recurrence and metastasis is the main cause of mortality in CRC patients, and the markers applied for diagnosis and treatment of CRC is still urgently needed to early diagnose and evaluate therapeutic effect. Long noncoding RNA (lncRNA) is a class of noncoding RNA that the length is more than 200 nucleotides. With the development of sequencing technique about transcriptome, increasing lncRNAs are focused on their function and mechanism related to the nosogenesis and pathology of CRC. Recent studies report that lncRNAs acted as crucial role in CRC and could be as biomarker for CRC diagnosis and treatment. In this review, we display the regulation of lncRNA by interacting with DNA, RNA and protein and highlight the double role of lncRNAs as oncogene or anti-tumor gene involved in Wnt signaling pathway, p53 signaling pathway or others to be an regulator in CRC development. Lastly, we discuss some new finding of lncRNAs, especially lncRNA in exosome, which could be as potential markers for diagnosis and treatment of CRC in future.

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Knockdown of linc-POU3F3 suppresses the proliferation, apoptosis, and migration resistance of colorectal cancer

Cited by 51 publications

References 50 publications

The lncRNA ZEB1-AS1 sponges miR-181a-5p to promote colorectal cancer cell proliferation by regulating Wnt/β-catenin signaling

The lncRNA ZEB1-AS1 sponges miR-181a-5p to promote colorectal cancer cell proliferation by regulating Wnt/β-catenin signaling

Comprehensive investigation of a novel differentially expressed lncRNA expression profile signature to assess the survival of patients with colorectal adenocarcinoma

The Biological Effect and Clinical Application of Long Noncoding RNAs in Colorectal Cancer

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