Knockdown of CYP6CR2 and CYP6DE5 reduces tolerance to host plant allelochemicals in the Chinese white pine beetle Dendroctonus armandi

Liu, Bin; Fu, Danyang; Ning, Hang; Tang, Ming; Chen, Hui

doi:10.1016/j.pestbp.2022.105180

Cited by 12 publications

(13 citation statements)

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“…In addition, NlAhR and NlARNT were induced by pesticides and participated in the NlCarE7 regulation, which was involved in the susceptibility to etofenprox and isoprocarb in N. lugens [ 48 ]. Previous studies have reported that CYP genes in D. armandi were remarkably increased in response to (−)-𝛼-pinene, (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene treatments [ 29 , 34 , 35 ]. Because knockdowns of DaCYP6DF 1 significantly increased D. armandi susceptibility to (−)-𝛽-pinene and (±)-limonene, we supposed that DaAhR and DaARNT regulate the susceptibility by targeting DaCYP6DF 1.…”

Section: Discussionmentioning

confidence: 99%

“…At the 48 h after dsRNA injection, the adults were treated with (−)-𝛼-pinene, (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene as described previously, then they were reared in normal conditions for 48 h and the mortality rates were determined. We selected 12 genes from the three classes of D. armandi detoxifying enzymes: 6 CYP genes from the CYP3 clade, 4 GST genes from epsilon and sigma superfamilies, and 2 carboxylesterases, which are involved in the xenobiotic compounds’ detoxification in D. armandi [ 34 , 35 ]. These detoxification gene expression levels ( P450, CarE, and GST ) after dsRNA injection at 48 h were measured by qRT-PCR.…”

Section: Methodsmentioning

confidence: 99%

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Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi

Liu

Chen

2022

Cells

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The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical substances, and subsequently activates genes transcription involved in various detoxification and physiological functions. However, they have not been identified in Dendroctonus armandi, and their roles in the detoxification metabolism are unclear. In the present study, AhR and ARNT of D. armandi were characterized. Spatiotemporal expression profiling indicated that DaAhR and DaARNT were highly expressed in the adult and larval stages of D. armandi and mainly expressed in the midgut and Malpighian tubules of adults. Additionally, the expression of DaAhR and DaARNT significantly increased after exposure to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene. Silencing DaAhR and DaARNT increased the susceptibility of D. armandi to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene, and the activities of detoxification enzyme were also remarkably reduced. Moreover, DaCYP6DF1 and DaGSTs2 were significantly down-regulated after injections of dsAhR and dsARNT in the male and female adults, with the expression of DaCYP6DF1 decreasing by higher than 70%. The present study revealed that the transcription factors AhR and ARNT of D. armandi were induced by terpenoids and participated in the regulation of DaCYP6DF1 expression, which was associated with D. armandi’s susceptibility to (−)-𝛽-pinene and (±)-limonene. These results may provide a theoretical basis for the integrated control of D. armandi and improve our comprehension of insect toxicology.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi

Liu

Chen

2022

Cells

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“…Pupae were separated into two substages: early pupae and late pupae. We separated the adults into three substages: teneral adults, emerged adults, and attacking adults [ 53 ]. The antennae, foregut, brain, midgut, fat body, hindgut, pheromone gland, Malpighian tubules, and hemolymph of emerged adults were stored at −80 °C.…”

Section: Methodsmentioning

confidence: 99%

Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 CYP4BQ1 Responsible for (+)-α-Pinene Tolerance in Dendroctonus armandi

Liu

Ming

Chen

2022

IJMS

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Bark beetles mainly rely on detoxification enzymes to resist the host tree’s defense against oleoresin terpenes. Cytochrome P450 enzymes (CYPs) play an important role in the detoxification of plant allelochemicals and pesticides in insect. One P450 gene (DaCYP4BQ1) is associated with the response of (+)-α-pinene in Dendroctonus armandi. However, the regulatory mechanism of this P450 gene response to (+)-α-pinene is still unknown. In this study, spatiotemporal expression profiling indicated that CYP4BQ1 was highly expressed in adult and larval stages of D. armandi, and it was predominantly expressed in fat body, midgut, and Malpighian tubules of adults. Moreover, the expression of CYP4BQ1 significantly increased after exposure to (+)-α-pinene, and depletion of it decreased the tolerance of adults to (+)-α-pinene. In addition, (+)-α-pinene treatment induced the expression of the transcription factors cap ‘n’ collar isoform C (CncC) and its binding factor muscle aponeurosis fibromatosis (Maf), elevated the level of hydrogen peroxide (H2O2), and increased the activities of antioxidant enzymes. Silencing CncC suppressed CYP4BQ1 expression and enhanced the susceptibility of beetles to (+)-α-pinene. Similarly, application of the reactive oxygen species (ROS) scavenger N-acetylcysteine reduced the production and accumulation of H2O2, suppressed the expression of CncC, Maf, and CYP4BQ1 and led to decreased tolerance of adults to (+)-α-pinene. In contrast, ingestion of the CncC agonist curcumin elevated CYP4BQ1 expression and enhanced (+)-α-pinene tolerance. The results demonstrate that, in D. armandi, (+)-α-pinene induces CYP4BQ1 via activation of the ROS/CncC signaling pathway.

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“…A recent study showed that the CYP6CR2 and CYP6DE5 genes were highly expressed under the treatment of terpenoids, whereas silencing of these genes significantly reduced the activity of P450 and increased the mortality of adults after exposure to terpenoids. CYP6CR2 expression was considerably higher in larvae and sclerotized adults, with lower expression in starving adult mountain pine beetles (Robert et al, 2013(Robert et al, , 2016Liu et al, 2022). A transcriptome study of the imidacloprid resistance (IR) and isogenic susceptible (IGS) strains of Aphis gossypii revealed that the resistant strain had high gene expression of cytochrome P450 4G15-like (Kim et al, 2015).…”

Section: A11(a) Cytochromes Involved In Detoxificationmentioning

confidence: 99%

Age matters: Life-stage, tissue, and sex-specific gene expression dynamics in Ips typographus (Coleoptera: Curculionidae: Scolytinae)

Naseer

Mogilicherla

Sellamuthu

et al. 2023

Front. For. Glob. Change

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The Eurasian spruce bark beetle (ESBB), Ips typographus, has recently caused catastrophic damage to Norway spruce (Picea abies) forests in Europe, resulting in the loss of more than 100 million cubic meters of wood. Traditional forest management strategies have failed to constrain the growing infestation rate; hence, novel measures must be deployed. A better understanding of ESBB physiology and adaptation to host allelochemicals may provide a platform for future management strategies using molecular tools such as RNA interference. To understand ESBB physiology and adaptation, the current study unraveled the gene expression dynamics of ESBB in different life stages and tissues. We obtained ESBB transcriptomes for different life stages [larvae (L1, L2, and L3), pupa, callow, and sclerotized adult] and male/female tissues (gut, fat body, and head) from callow and sclerotized adult beetles. Differential gene expression analysis (DGE) identified multiple gene families related to detoxification, digestion, resistance, and transport in different life stages and tissues of the beetle. Gene Ontology (GO) enrichment revealed 61 critical metabolic pathways enriched across all DGE comparisons. DGE analysis further pinpointed the differential expression of essential genes involved in detoxification, digestion, transport, and defense in various tissues and life stages. RT-qPCR experiments and enzymatic assays corroborated the findings further. The catalogue of differentially expressed genes identified in ESBB could aid better understanding of ESBB physiology and adaptation to hosts and serve as targets for future RNAi-based ESBB management.

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Knockdown of CYP6CR2 and CYP6DE5 reduces tolerance to host plant allelochemicals in the Chinese white pine beetle Dendroctonus armandi

Cited by 12 publications

References 60 publications

Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi

Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi

Activation of the ROS/CncC Signaling Pathway Regulates Cytochrome P450 CYP4BQ1 Responsible for (+)-α-Pinene Tolerance in Dendroctonus armandi

Age matters: Life-stage, tissue, and sex-specific gene expression dynamics in Ips typographus (Coleoptera: Curculionidae: Scolytinae)

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