Identification and Functional Characterization of the Transcription Factors AhR/ARNT in Dendroctonus armandi

Abstract

The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical substances, and subsequently activates genes transcription involved in various detoxification and physiological functions. However, they have not been identified in Dendroctonus armandi, and their roles in the detoxification metabolism are unclear. In the present study, AhR and ARNT of D. armandi were characterized. Spatiotemporal expression profiling indicated that DaAhR and DaARNT were highly expressed in the adult and larval stages of D. armandi and mainly expressed in the midgut and Malpighian tubules of adults. Additionally, the expression of DaAhR and DaARNT significantly increased after exposure to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene. Silencing DaAhR and DaARNT increased the susceptibility of D. armandi to (−)-𝛽-pinene, (+)-3-carene, and (±)-limonene, and the activities of detoxification enzyme were also remarkably reduced. Moreover, DaCYP6DF1 and DaGSTs2 were significantly down-regulated after injections of dsAhR and dsARNT in the male and female adults, with the expression of DaCYP6DF1 decreasing by higher than 70%. The present study revealed that the transcription factors AhR and ARNT of D. armandi were induced by terpenoids and participated in the regulation of DaCYP6DF1 expression, which was associated with D. armandi’s susceptibility to (−)-𝛽-pinene and (±)-limonene. These results may provide a theoretical basis for the integrated control of D. armandi and improve our comprehension of insect toxicology.

Transcription factor AhR regulates glutathione S-transferases (GSTs) conferring resistance tolambda-cyhalothrin inCydia pomonella

“…The copyright holder for this preprint this version posted January 3, 2023. ; https://doi.org/10.1101/2023.01.03.522531 doi: bioRxiv preprint participated in the regulation of DaCYP6DF1 expression, which was associated with susceptibility to (−)-β-pinene and (±)-limonene [24]. Therefore, clarifying the regulatory relationship between the transcription factor AhR and detoxification enzymes is critical for better understanding resistance mechanisms to insecticides and provides important clues for improving sustainable pest management.…”

“…Knockdown of the expression of the AhR gene decreased the P450 enzyme activity and reduced the tolerance of Nilaparvata lugens to several insecticides [23]. The transcription factor AhR of Dendroctonus armandi was induced by terpenoids and participated in the regulation of DaCYP6DF1 expression, which was associated with susceptibility to (−)-β-pinene and (±) -limonene [24]. Therefore, clarifying the regulatory relationship between the transcription factor AhR and detoxification enzymes is critical for better understanding resistance mechanisms to insecticides and provides important clues for improving sustainable pest management.…”

Transcription Factor AhR Regulates Glutathione S-Transferases Conferring Resistance to lambda-Cyhalothrin in Cydia pomonella

“…Knockdown of the expression of the AhR gene decreased the P450 enzyme activity and reduced the tolerance of fourth-instar nymphs to several insecticides in Nilaparvata lugens . The transcription factor AhR of Dendroctonus armandi was associated with susceptibility to (−)-β-pinene and (±)-limonene by the regulation of DaCYP6DF1 expression . Therefore, elucidating the regulatory relationship between the transcription factor AhR and detoxification enzyme genes is critical for better understanding the resistance mechanisms to insecticides and provides important clues for improving sustainable pest management.…”

“…23 The transcription factor AhR of Dendroctonus armandi was associated with susceptibility to (−)-β-pinene and (±)-limonene by the regulation of DaCYP6DF1 expression. 24 Therefore, elucidating the regulatory relationship between the transcription factor AhR and detoxification enzyme genes is critical for better understanding the resistance mechanisms to insecticides and provides important clues for improving sustainable pest management. However, the transcription factor AhR has not been identified and its regulatory role in the transcription of detoxification genes is unknown in C. pomonella.…”

Transcription Factors AhR and ARNT Regulate the Expression of CYP6SX1 and CYP3828A1 Involved in Insecticide Detoxification in Bradysia odoriphaga

“…35 The transcription factors AhR and ARNT regulated the expression of DaCYP6DF1 associated with Dendroctonus armandi adults susceptibility to (−)-β-pinene and (±)-limonene. 36 Therefore, exploring the transcriptional regulatory network can better understand the molecular regulation mechanism of detoxification genes, which is crucial for resistance management. However, the regulatory role of transcription factors AhR and ARNT in detoxification genes remains unclear in B. odoriphaga.…”