Knockdown of CDCA8 inhibits the proliferation and enhances the apoptosis of bladder cancer cells

Abstract

Bladder cancer is a tumour of the urinary system with high mortality, and there is also a great lack of therapeutic targets in the clinic. Cell division cycle associated 8 (CDCA8), an important component of the vertebrate chromosomal passenger complex, is highly expressed in various tumours and promotes tumour development. However, the role of CDCA8 in bladder cancer is not fully understood. This study aimed to reveal the function of CDCA8 in bladder cancer by determining the relationship between CDCA8 expression and proliferation, metastasis and apoptosis of bladder cancer cells. Firstly, we studied the mRNA expression of CDCA8 through the Gene Expression Omnibus (GEO) and the Cancer Genome Atlas (TCGA) databases and analysed the correlation between CDCA8 expression and prognosis of patients with bladder cancer. We also verified CDCA8 expression in bladder cancer tissues by immunohistochemistry. In addition, CDCA8 expression was inhibited in bladder cancer T24 and 5637 cells, and the effects of CDCA8 on the proliferation, migration and invasion of bladder cancer cell lines were investigated using cell counting kit-8, colony formation, cell cycle, apoptosis, wound healing and Transwell invasion assays. Results showed that CDCA8 was highly expressed in bladder cancer compared with normal tissues, and the high CDCA8 expression was significantly correlated with the poor prognosis of patients. Inhibiting CDCA8 expression inhibited the proliferation, migration and invasion of T24 and 5637 cells and induced the apoptosis of bladder cancer cells. CDCA8 was involved in the regulation of the growth cycle of bladder cancer cells. Bioinformatics-based mechanism analysis revealed that high CDCA8 expression may affect the cell cycle and P53 signalling pathways. In conclusion, our results suggest that CDCA8 is highly expressed in bladder cancer and can promote tumour development. Hence, CDCA8 may serve as an effective therapeutic target for treatment of bladder cancer.

Silencing CDCA8 inhibits the proliferation and invasion of gastric cancer cells and induces apoptosis by blocking the Akt pathway

“…In terms of cell growth, this study revealed, through CCK-8 and colony formation assays, that knockdown of CDCA8 inhibited GC cell viability and proliferation ability. This result is consistent with previous reports that CDCA8 knockdown inhibited the proliferation of skin melanoma cell lines [17], and bladder [18] and breast cancer [19]. Can et al [20] found that the CDCA8 promoter has high activity in several cancer cell lines, as well as in undifferentiated human embryonic stem cells (hESCs) and early mouse embryos, which share the common feature of retaining high proliferative activity.…”

The Higher Expression of CDCA2 Associated with Poor Prognosis in Glioma

“…CDCA4 has been reported to be a potential biomarker for clinical outcome of osteosarcoma patients [ 7 ]. In many several studies, CDCA7 and CDCA8 are demonstrated to be highly expressed in enteritis cancer [ 8 , 9 ]. Their excessive expression is obviously related to CRC invasion depth, lymph gland, tumor node, and distant metastasis.…”

Double-targeting CDCA8 and E2F1 inhibits the growth and migration of malignant glioma

“…And Dai et al recognized CDCA8 as a downstream target of transcriptional activation by transcription factor NF-Y in both human embryonic stem cells and cancer cells, demonstrating the potential regulatory mechanism of CDCA8 21 . Besides, abnormal expression and positive regulatory functions of CDCA8 in clear cell renal cell carcinoma and bladder cancer have also been illuminated 26 , 27 . In our study, we found that CDCA8 promoted glioma cell proliferation by inhibiting cell apoptosis and cell cycle arrest, and enhanced cell migration.…”