Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

Ali, Asfa; Bovilla, Venugopal R.; Mysarla, Danti Kumari; Siripurapu, Prasanthi; Pathak, Rashmi U.; Basu, Bhakti; Mamillapalli, Anitha; Bhattacharya, Santanu

doi:10.1038/s41598-017-00653-3

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…This is consistent with previous literature which shows that actively transcribed genes are associated with the nuclear matrix (65). Since the broad complex was shown to negatively regulate FIBH expression (66), the Br-c gene was analysed for MARs. Though matrix attachment in intergenic regions can protect genes from being silenced, the presence of MARs within genes correlates with gene silencing (69,95).…”

Section: Discussionsupporting

confidence: 90%

“…Previous work from our lab showed that the broad-complex negatively regulates broin gene expression (66).This was further supported by a study by a recent study that showed that the overexpression of BmBr-c Z2 in the transgenic lines of B. mori led to decline in the FIBH, Fib-l and P25 silk proteins. This mechanism was shown to be connected with the juvenile hormone pathway which is linked to the expression of its transcription factors, Bmdimm and BmKr-h1 (67).…”

Section: Dynamic Attachment Of Fibh and Br-c Regions With The Nuclear...supporting

confidence: 64%

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Dynamics of Nuclear Matrix Attachment Regions During 5th Instar Posterior Silk Gland Development in Bombyx Mori

Chunduri

Rajan

Lima

et al. 2022

Preprint

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Chromatin architecture is critical for gene expression during development. Matrix attachment regions (MARs) control and regulate chromatin dynamics. The position of MARs in the genome determines the expression of genes in the organism. In this study, we set out to elucidate how MARs temporally regulate the expression of the fibroin heavy chain (FIBH) gene during development. We addressed this by identifying MARs and studying their distribution and differentiation, in the posterior silk glands of Bombyx mori during 5th instar development. Of the MARs identified on three different days, 7.15% MARs were common to all three, whereas, 1.41%, 19.27% and 52.47% MARs were unique to day 1, day 5, and day 7, respectively highlighting the dynamic nature of the matrix associated DNA. Further, significant changes in the MARs in the vicinity of the FIBH gene were found during different days of 5th instar development which implied their role in the regulation and expression of the FIBH gene.

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Section: Discussionsupporting

confidence: 90%

Section: Dynamic Attachment Of Fibh and Br-c Regions With The Nuclear...supporting

confidence: 64%

Dynamics of Nuclear Matrix Attachment Regions During 5th Instar Posterior Silk Gland Development in Bombyx Mori

Chunduri

Rajan

Lima

et al. 2022

Preprint

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“…According to our previous transcriptomic analysis of the silk glands from molting to inter-molting stages, BmBrC-Zs were significantly overexpressed, but the expression of the fibroin genes at the molting stage was almost absent [11]. Synthetic oligopyrrole carboxamides can downregulate the expression levels of various isoforms of BmBrC gene in the silk gland, which resulted in statistically enhanced cocoon weight, shell weight and silk yield [41]. Therefore, we hypothesized that BmBrC may be involved in the negative regulation of fibroin genes.…”

Section: Introductionmentioning

confidence: 97%

Transgenic Ectopic Overexpression of Broad Complex (BrC-Z2) in the Silk Gland Inhibits the Expression of Silk Fibroin Genes of Bombyx mori

Cong

Tao

Zhang

et al. 2020

Insects

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Bombyx mori silk protein genes are strictly turned on and off in different developmental stages under the hormone periodically change. The broad complex (BrC) is a transcription factor mediating 20-hydroxyecdysone action, which plays important roles during metamorphosis. Here, we observed that two isoforms of BmBrC (BmBrC-Z2 and BmBrC-Z4) exhibited contrasting expression patterns with fibroin genes (FibH, FibL and P25) in the posterior silk gland (PSG), suggesting that BmBrC may negatively regulate fibroin genes. Transgenic lines were constructed to ectopically overexpress BmBrC-Z2 in the PSG. The silk protein genes in the transgenic line were decreased to almost half of that in the wild type. The silk yield was decreased significantly. In addition, the expression levels of regulatory factors (BmKr-h1 and BmDimm) response to juvenile hormone (JH) signal were inhibited significantly. Then exogenous JH in the BmBrC-Z2 overexpressed lines can inhibit the expression of BmBrC-Z2 and activate the expression of silk protein genes and restore the silk yield to the level of the wild type. These results indicated that BmBrC may inhibit fibroin genes by repressing the JH signal pathway, which would assist in deciphering the comprehensive regulation mechanism of silk protein genes.

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“…Combining with population genomics and developmental transcriptomic data of the silk gland, integrated analyses were applied to reveal those yield-related loci. It provides a good opportunity for exploring yield-related genes and molecular mechanisms in domestic and wild silkworms, which might be used for high-yield breeding with genome editing, genetic knockdown and overexpression technologies 29,30 .…”

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confidence: 99%

Genetic and genomic analysis for cocoon yield traits in silkworm

Fang

Zhou

et al. 2020

Sci Rep

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Domestic species provides a powerful model for examining genetic mechanisms in the evolution of yield traits. the domestic silkworm (Bombyx mori) is an important livestock species in sericulture. While the mechanisms controlling cocoon yield are largely unknown. Here, using B. mori and its wild relative B. mandarina as intercross parents, 100 BC 1 individuals were sequenced by restriction site-associated DNA sequencing (RAD-Seq). The linkage map contained 9,632 markers was constructed. We performed high-resolution quantitative trait locus (QTL) mapping for four cocoon yield traits. A total of 11 QTLs were identified, including one yield-enhancing QTL from wild silkworm. By integrating population genomics and transcriptomic analysis with QTLs, some favourable genes were revealed, including 14 domestication-related genes and 71 differentially expressed genes (DEGs) in the fifth-instar larval silk gland transcriptome between B. mori and B. mandarina. the relationships between the expression of two important candidate genes (KWMTBOMO04917 and KWMTBOMO12906) and cocoon yield were supported by quantitative real-time pcR (qpcR). our results provide some new insights into the molecular mechanisms of complex yield traits in silkworm. The combined method might be an efficient approach for identifying putative causal genes in domestic livestock and wild relatives.

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Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

Cited by 4 publications

References 58 publications

Dynamics of Nuclear Matrix Attachment Regions During 5th Instar Posterior Silk Gland Development in Bombyx Mori

Dynamics of Nuclear Matrix Attachment Regions During 5th Instar Posterior Silk Gland Development in Bombyx Mori

Transgenic Ectopic Overexpression of Broad Complex (BrC-Z2) in the Silk Gland Inhibits the Expression of Silk Fibroin Genes of Bombyx mori

Genetic and genomic analysis for cocoon yield traits in silkworm

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