Genetic and genomic analysis for cocoon yield traits in silkworm

Fang, Shou-Min; Zhou, Qi; Yu, Qiuhan; Zhang, Ze

doi:10.1038/s41598-020-62507-9

Cited by 12 publications

(12 citation statements)

References 63 publications

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“…Under long-term artificial selection, B. mori acquired various characteristics suitable for sericulture. For example, the weight of the cocoon shell of B. mori is much higher than that of B. mandarina (Ômura 1950, Li et al 2017, Fang et al 2020). In addition, B. mori moths lost their flying ability due to the degeneration of their flight muscles and a reduction in wing stiffness (Lu et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The issue of lethality is likely to continue to impede the genetic analysis of pigmentation loss and other domestication-associated traits in B. mori. Recently, several candidate genes associated with domestication in B. mori have been identified using quantitative trait locus (QTL) mapping, including silk production (Li et al 2017, Fang et al 2020, larval climbing ability (Wang, Lin, et al 2020), and mimicry (Wang, Lin, et al 2020). In addition, a recent population genetic analysis identified 300 candidate genes as targets of recent selection in B. mori, some of which are likely to be associated with silk production and voltinism (Xiang et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, several candidate genes associated with domestication in B. mori have been identified using quantitative trait locus (QTL) mapping, including silk production (Li et al . 2017, Fang et al . 2020), larval climbing ability (Wang, Lin, et al .…”

Section: Introductionmentioning

confidence: 99%

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Allele-specific knockouts reveal a role for apontic-like in the evolutionary loss of larval pigmentation in the domesticated silkworm, Bombyx mori

Tomihara

Andolfatto

Kiuchi

2022

Preprint

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The domesticated silkworm, Bombyx mori, and its wild progenitor, B. mandarina, are extensively studied as a model case of the evolutionary process of domestication. A conspicuous difference between these species is the dramatic reduction in pigmentation in both larval and adult B. mori. Here we evaluate the efficiency of CRISPR/Cas9-targeted knockouts of pigment-related genes as a tool to understand their potential contributions to domestication-associated pigmentation loss in B. mori. To demonstrate the efficacy of targeted knockouts in B. mandarina, we generated a homozygous CRISPR/Cas9-targeted knockout of yellow-y. In yellow-y knockout mutants, black body color became lighter throughout the larval, pupal and adult stages, confirming a role for this gene in pigment formation. Further, we performed allele-specific CRISPR/Cas9-targeted knockouts of the pigment-related transcription factor, apontic-like (apt-like) in B. mori × B. mandarina F1 hybrid individuals. Knockout of the B. mandarina allele of apt-like in F1 embryos results in depigmented patches on the dorsal integument of larvae, whereas corresponding knockouts of the B. mori allele consistently exhibit normal F1 larval pigmentation. These results demonstrate a contribution of apt-like to the evolution of reduced pigmentation in B. mori. Together, our results demonstrate the feasibility of CRISPR/Cas9-targeted knockouts as a tool for understanding the genetic basis of traits associated with B. mori domestication.Brief abstractBombyx mori and its wild progenitor are an important model for the study of phenotypic evolution associated with domestication. As proof-of-principle, we used CRISPR/Cas9 to generate targeted knockouts of two pigmentation-related genes. By generating a homozygous knockout of yellow-y in B. mandarina, we confirmed this gene”s role in pigment formation. Further, by generating allele-specific knockouts of apontic-like (apt-like) in B. mori × B. mandarina F1 hybrids, we establish that evolution of apt-like contributed to reduced pigmentation during B. mori domestication.Graphical TOC/Abstract

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Allele-specific knockouts reveal a role for apontic-like in the evolutionary loss of larval pigmentation in the domesticated silkworm, Bombyx mori

Tomihara

Andolfatto

Kiuchi

2022

Preprint

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“…In recent years, transcriptome and proteomics methods have been used to screen genes related to CSW, which plays an important role in guiding the study of genes related to silk yield (Ma et al 2014;Wang et al 2014;Fang et al 2015;Li et al 2016;Peng 2016;Luan et al 2018). Fang et al also screened related genes that regulate the silk yield through genomic analysis (Fang et al 2020). However, owing to the uncertainty of omics analysis, researchers are still trying to map the major genes through forward genetics.…”

Section: Introductionmentioning

confidence: 99%

Gene mapping reveals the association between tyrosine protein kinase Abl1 and the silk yield of Bombyxmori

Luan

Zuo

et al. 2021

Animal Genetics

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The Z chromosome of the silkworm contains a major gene that influences silk yield. This major locus on chromosome Z accounts for 35.10% of the phenotypic variance. The location and identification of the gene have been a focus of silkworm genetics research. Unfortunately, identification of this gene has been difficult. We used extreme phenotype subpopulations and selected from a backcross population, BC 1 M, which was obtained using the high-yield strain 872B and the low-yield strain IS-Dazao as parents, for mapping the gene on the chromosome Z. The candidate region was narrowed down to 134 kb at the tip of the chromosome. BmAbl1 in this region correlated with silk gland development by spatiotemporal expression analysis. This gene was differentially expressed in the posterior silk glands of the high-and low-yield strains. In BmAbl1, an insertion-deletion (indel) within the 10th exonic region and an SNP within the 6th intronic region were detected and shown to be associated with cocoon shell weight in 84 Bombyx mori strains with different yields. Nucleotide diversity analysis of BmAbl1 and its 50 kb flanking regions indicated that BmAbl1 has experienced strong artificial selection during silkworm domestication. This study is the first to identify the genes controlling silk yield in the major QTL of the Z chromosome using forward genetics.

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“…Research shown that silkworms may have been initially domesticated in China as tri-moulting lines, then subjected to independent spreads along the Silk Road that gave rise to the development of most local strains, and further improved for modern silk production in Japan and China, having descended from diverse ancestral sources (Lu et al, 2002;Pan et al, 2008;Sun et al, 2012;Xia et al, 2009;Xiang et al, 2018). Without artificial selection and live in the wild, wild silkworm preserved abundant genetic diversity, and shown significant differences in cocoon quality, disease resistance, stress tolerance, growth and development when compared with domestic silkworm, so it could be germplasm resources of genetic improvement of domestic silkworm (Fang et al, 2020;Fang et al, 2015;Xiang et al, 2013;Zhang et al, 2015). The origin and domestication of Bombyx mori and Bombyx mandarina are basic scientific issues in sericulture, and also involve in the inheritance of the silk culture.…”

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confidence: 99%

Genome Survey and Mitochondrial Genome Analysis of Wild Silkworm, <i>Bombyx mandarina</i>

Ma¹,

Wang²,

Qu³

2020

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Aimed to lay references for the whole genome sequencing and provide basic data for the genetic relationships between Bombyx mandarina and Bombyx mori, the genome of Bombyx mandarina is investigated and the mitochondrial genome is analyzed. Using the Illumina NovaSeq pair-end sequencing platform, a female Bombyx mandarina was sequenced, the genome size, heterozygosity and GC contend have been estimated based on the genomic survey analysis, and the genome of wild silkworm has been pre-assembled. Using the sequencing data, the mitochondrial genome sequences in the genome were extracted and assembled directly. As results, 25.8 GB clean data obtained, the estimated genome size of Bombyx mandarina is 456.5 Mb, the heterozygosity rate is 1.94%. After preliminary assembly, the scaffold N50 is 1 792 bp, scaffold number is 737055, contig N50 is 587 bp, contig number is 1477268. As assembly and annotation, the mitochondrial genome of wild silkworm is 15 662 bp, a total of 37 genes were arranged in the mitochondrial genome, which did not have a gene rearrangement. According the phylogenetic tree of mitochondrial genome, wild silkworm could be divided into north or south group according to the geographical source. Subgroup of Wild silkworm from the North China has the closest genetical relationship with the domestic silkworm. Since the genome of Qin-ba wild silkworm belong to the complex genome, integrating the second-, the third-generation sequencing and Hi-C technology could be helpful to obtain high quality genomic of Bombyx mandarina. This study also supports the contention that domestic of silkworm descended from the northern of China, and implied that the Qin-ba mountain area could be one of the original regions of domestic silkworm.

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Genetic and genomic analysis for cocoon yield traits in silkworm

Cited by 12 publications

References 63 publications

Allele-specific knockouts reveal a role for apontic-like in the evolutionary loss of larval pigmentation in the domesticated silkworm, Bombyx mori

Allele-specific knockouts reveal a role for apontic-like in the evolutionary loss of larval pigmentation in the domesticated silkworm, Bombyx mori

Gene mapping reveals the association between tyrosine protein kinase Abl1 and the silk yield of Bombyxmori

Genome Survey and Mitochondrial Genome Analysis of Wild Silkworm, <i>Bombyx mandarina</i>

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