Gene mapping reveals the association between tyrosine protein kinase Abl1 and the silk yield of Bombyxmori

Abstract

The Z chromosome of the silkworm contains a major gene that influences silk yield. This major locus on chromosome Z accounts for 35.10% of the phenotypic variance. The location and identification of the gene have been a focus of silkworm genetics research. Unfortunately, identification of this gene has been difficult. We used extreme phenotype subpopulations and selected from a backcross population, BC 1 M, which was obtained using the high-yield strain 872B and the low-yield strain IS-Dazao as parents, for mapping the gene on the chromosome Z. The candidate region was narrowed down to 134 kb at the tip of the chromosome. BmAbl1 in this region correlated with silk gland development by spatiotemporal expression analysis. This gene was differentially expressed in the posterior silk glands of the high-and low-yield strains. In BmAbl1, an insertion-deletion (indel) within the 10th exonic region and an SNP within the 6th intronic region were detected and shown to be associated with cocoon shell weight in 84 Bombyx mori strains with different yields. Nucleotide diversity analysis of BmAbl1 and its 50 kb flanking regions indicated that BmAbl1 has experienced strong artificial selection during silkworm domestication. This study is the first to identify the genes controlling silk yield in the major QTL of the Z chromosome using forward genetics.

Comparative analysis of quantitative phosphoproteomics between two tilapias (Oreochromis niloticus and Oreochromis aureus) under low-temperature stress

“…The proteins involved in these BP terms included SH3, multiple ankyrin repeat domains protein 3 and tyrosine-protein kinase ABL1 (see Table S2 ). The function of these proteins is mainly related to nerve development, cell division and growth ( Kim et al, 2020 ; Luan et al, 2021 ). Together, our results indicated that the physiological processes involved in the low temperature tolerance acted differently in the two tilapias.…”

BmAbl1 Regulates Silk Protein Synthesis via Glutathione Metabolism in Bombyx mori

“…BmAbl1 is one of the potential targets in candidate regions. A recent report suggested that BmAbl1 had a strong correlation with cocoon shell weight [9,10]. However, how BmAbl1 affects the synthesis of silk protein is still unknown.…”

“…Insects 2022, 13, 967 2 of 13 Within this QTL region, the tyrosine protein kinase, BmAbl1, is considered a potential target gene affecting CSW [9,10].…”

A Cluster of Genes at the Terminal Region of Chromosome 1 Contributes to Silk Yield in Bombyx Mori.

“…In the previous work, our research group used linkage analysis to circumvent the effects of gender in order to locate a quantitative trait loci (QTL) at 0-134k of the 1st chromosome (Chr1) and found that BmAbl1 (BMgn002070) was signi cantly related to the CSW in this region [11]. In the course of this research, we preliminarily found that the mapping region seemed to have been selected as a whole unit.…”

“…However, because the silkworm has complex genetic characteristics such as female complete linkage and other factors that could affect yield-related indicators, it is di cult to formulate suitable mapping materials and methods in forward genetic studies, making it di cult to research the genetic basis of silk yield. Therefore, over the past 100 years, only a few major genes have been detected, including BmGlcNase1 and BmAbl1 [11,12]. Since the inheritance of silk yield traits is dominated by additive effects [13] and the few identi ed genes have limited effects on molecular breeding, it is necessary to identify additional silk yield controlling genes.…”