klumpfuss regulates cell death in the Drosophila retina

Rusconi, Jamie C.; Fink, Jill L.; Cagan, Ross L.

doi:10.1016/j.mod.2004.04.008

Cited by 29 publications

(29 citation statements)

References 39 publications

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“…Many genes involved in the apoptotic process have been identified, for example, reaper, Dronc, DmPpt1, klumpfuss. Overexpression of these genes induced small eyes and black ommatidia 25,28,38,39 . Therefore, we also suggest that the Ten-a protein might be another protein involved in the cell death mechanism of the eye.…”

Section: Ten-a Ten-a Ten-a Ten-a Ten-a Is Required For Commissure Formentioning

confidence: 99%

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Ngernsiri¹,

Fascetti²,

Wisorum³

et al. 2005

ScienceAsia

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ten-a is one of the two Drosophila genes whose products belong to the Ten M protein family. The Ten-a protein is a type II transmembrane protein. This protein was found to be mainly expressed in the CNS of the embryo and in the compound eye of the pupa. No report about the function of ten-a has been published to date. Here, we showed, in a preliminary report, that ten-a has a function in the development of the CNS and in the compound eye. To study the function of ten-a during CNS development, doublestranded RNA interference (dsRNAi) experiments were performed. We found that embryos without Ten-a protein have defects in the commissures, suggesting that ten-a is likely to be involved in commissure formation. Furthermore, to study the function of ten-a in the eye, the Gal4/UAS system was used to overexpress ten-a at different stages of eye development. We found that extra amounts of Ten-a can interfere with the development of the eye. Flies overexpressing ten-a showed eye defects such as small and rough eyes, and black ommatidia. The results indicated that ten-a may be a novel gene involved in eye morphogenesis.

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Section: Ten-a Ten-a Ten-a Ten-a Ten-a Is Required For Commissure Formentioning

confidence: 99%

“…The system has been used successfully to investigate the function of many genes 25,28,37,38 . With this system, a wild-type tena transgene was overexpressed in the eye by using three different Gal4 driver lines.…”

Section: Ten-a Ten-a Ten-a Ten-a Ten-a Is Required For Commissure Formentioning

confidence: 99%

Untitled

Ngernsiri¹,

Fascetti²,

Wisorum³

et al. 2005

ScienceAsia

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“…identified WT-1 binding sites upstream of evenskipped (eve) and showed that these sites are regulated by klu (McDonald et al, 2003). We have previously shown that klu is both necessary and sufficient for developmentally-regulated retinal apoptosis through regulation of dEGFR/Ras pathway activity (Rusconi et al, 2004). In addition, our previous work has shown that klu is the first and to date only molecule identified that is differentially expressed in the interommatidial cells or secondary and tertiary pigment cells, i.e., the cells that choose to live or die, prior to activation of apoptosis in the developing retina (Rusconi et al, 2004).…”

Section: Abstract: Drosophila Retina Btb/poz Domain Mesoderm Apomentioning

confidence: 99%

“…In situ hybridization of retina was performed similarly to Rusconi, Fink and Cagan (Rusconi et al, 2004). The one exception being that probes were labeled with fluorescence eliminating the need for secondary antibodies to visualize localization.…”

Section: In Situ Hybridization and Antibody Staining Of Larval And Pumentioning

confidence: 99%

Drosophila Mrityu encodes a BTB/POZ domain-containing protein and is expressed dynamically during development

Rusconi¹,

Challa²

2007

Int. J. Dev. Biol.

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We report the identification and characterization of expression of a new gene in Drosophila, which we have named Mrityu (Mri). Mri was initially isolated in a microarray screen to identify molecules regulated by the transcription factor klumpfuss during retinal apoptosis. The amino acid sequence of Mri contains a BTB/POZ domain with homologues across the animal kingdom. Mri transcripts are present at every developmental stage as assayed by RT-PCR. We show expression of Mri transcripts in the female germline, confined to the nurse cells, beginning at stage 7/8. During embryonic development Mri is uniformly expressed early and then is refined to the gut and mesoderm primordia while expression decreases in the ectoderm. In the retina Mri is again expressed uniformly early, i.e., in the third instar larva and becomes more refined during pupal development where the transcripts is dynamically expressed in the cone cells and primary, secondary and tertiary pigment cells.

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“…A transcription factor, Klumpfuss, is differentially expressed in IOCs during pupal stages when selection occurs. Loss of function of klumpfuss specifically in IOCs is associated with extra pigment cells (Rusconi et al, 2004). Genetic and molecular analysis indicates that Klumpfuss represses EGFR signaling in IOCs (Wildonger et al, 2005).…”

Section: Introductionmentioning

confidence: 99%