Drosophila Mrityu encodes a BTB/POZ domain-containing protein and is expressed dynamically during development

Rusconi, Jamie C.; Challa, Uma

doi:10.1387/ijdb.062233jr

Cited by 3 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S3). One candidate heart gene tested was CG1216 (Mrityu), a mesoderm-expressed BTB-POZ domain containing protein (Rusconi and Challa, 2007). Cardiac knock-down of CG1216 resulted in a significant increase in systolic diameter.…”

Section: Resultsmentioning

confidence: 99%

A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function

Neely

Kuba

Cammarato

et al. 2010

Cell

194

225

View full text Add to dashboard Cite

SUMMARY Heart diseases are the most common causes of morbidity and death in humans. Using cardiac-specific RNAi-silencing in Drosophila, we knocked-down 7061 evolutionarily conserved genes under conditions of stress. We present a first global road-map of pathways potentially playing conserved roles in the cardiovascular system. One critical pathway identified was the CCR4-Not complex implicated in transcriptional and post-transcriptional regulatory mechanisms. Silencing of the CCR4-Not components in adult Drosophila resulted in myofibrillar disarray and dilated cardiomyopathy. Heterozygous not3 knockout mice showed spontaneous impairment of cardiac contractility and increased susceptibility to heart failure. These heart defects were reversed via inhibition of HDACs suggesting a mechanistic link to epigenetic chromatin remodeling. In humans, we show that a common NOT3 SNP correlates with altered cardiac QT intervals, a known cause of lethal arrhythmias. Thus, our functional genome-wide screen in Drosophila can identify candidates that directly translate into conserved mammalian genes involved in heart function.

show abstract

Section: Resultsmentioning

confidence: 99%

A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function

Neely

Kuba

Cammarato

et al. 2010

Cell

194

225

View full text Add to dashboard Cite

show abstract

“…The mri gene encodes a protein that is structurally similar to the tetramerization domain of voltage activated potassium channels [39]. NMDA receptors are well-known ionotropic glutamate receptors that form heteromeric channel complexes comprised of essential Nmdar1 subunits and Nmdar2 subunits [40].…”

Section: Resultsmentioning

confidence: 99%

Dendritic Filopodia, Ripped Pocket, NOMPC, and NMDARs Contribute to the Sense of Touch in Drosophila Larvae

2012

View full text Add to dashboard Cite

Summary Background Among the Aristotelian senses, the subcellular and molecular mechanisms involved in the sense of touch are the most poorly understood. Results We demonstrate that specialized sensory neurons, the class II and class III multidendritic (md) neurons, are gentle touch sensors of Drosophila larvae. Genetic silencing of these cells significantly impairs gentle touch responses, optogenetic activation of these cells triggers behavioral touch-like responses, and optical recordings from these neurons show that they respond to force. The class III neurons possess highly dynamic dendritic protrusions rich in F-actin. Genetic manipulations that alter actin dynamics indicate that the actin-rich protrusions (termed sensory filopodia) on the class III neurons are required for behavioral sensitivity to gentle touch. Through a genome-wide RNAi screen of ion channels, we identified Ripped Pocket (rpk), No Mechanoreceptor Potential C (nompC), and NMDA Receptors 1 and 2 (Nmdars) as playing critical roles in both behavioral responses to touch and in the formation of the actin-rich sensory filopodia. Consistent with this requirement, reporters for rpk and nompC show expression in the class III neurons. A genetic null allele of rpk confirms its critical role in touch responses. Conclusions Output from class II and III md neurons of the Drosophila larvae is necessary and sufficient for eliciting behavioral touch responses. These cells show physiological responses to force. Actin-rich organelles in the Class III neurons are required for gentle touch detection. Ion channels in several force-sensing gene families are required in these cells, both for behavioral sensitivity to touch, and for the formation of the actin-rich sensory filopodia.

show abstract

“…Interestingly, the observation that genes encoding a putative BTB/ POZ domain containing protein are expressed in the nurse cells is not new. The Drosophila Mrityu, that has been demonstrated to participate in retinal apoptosis, is expressed throughout all stages of Drosophila development and in the nurse cells during oogenesis (Rusconi and Challa, 2007).…”

Section: Discussionmentioning

confidence: 99%

Expression study of an α-l-fucosidase gene in the Drosophilidae family

Pasini

Intra

Pavesi

2008

Gene

View full text Add to dashboard Cite

Drosophila Mrityu encodes a BTB/POZ domain-containing protein and is expressed dynamically during development

Cited by 3 publications

References 21 publications

A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function

A Global In Vivo Drosophila RNAi Screen Identifies NOT3 as a Conserved Regulator of Heart Function

Dendritic Filopodia, Ripped Pocket, NOMPC, and NMDARs Contribute to the Sense of Touch in Drosophila Larvae

Expression study of an α-l-fucosidase gene in the Drosophilidae family

Contact Info

Product

Resources

About