Klotho upregulation contributes to the neuroprotection of ligustilide against cerebral ischemic injury in mice

Long, Fangyi; Shi, Meng-Qi; Zhou, Hongjing; Liu, Dongling; Sang, Na; Du, Jun-Rong

doi:10.1016/j.ejphar.2017.12.019

Cited by 29 publications

(19 citation statements)

References 29 publications

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“…Various signalling pathways (Lang et al, 2020), including Wnt/β‐catenin, Notch, PI3K‐Akt and ERK1/2, participate in NSCs proliferation or differentiation. In addition, studies have demonstrated that LIG is involved in the regulation of ERK phosphorylation (Wu et al, 2011), Toll‐like receptor 4 (TLR4) (Qian et al, 2016), Akt and FoxO1 phosphorylation (Long et al, 2018). Thus, we further explored the expression level of these proteins in isolated H‐NSCs cultured with or without LIG treatment (72 h) in vitro.…”

Section: Resultsmentioning

confidence: 99%

Ligustilide enhances hippocampal neural stem cells activation to restore cognitive function in the context of postoperative cognitive dysfunction

Sun

Zhou

et al. 2021

Eur J of Neuroscience

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Ligustilide exerts potential neuroprotective effects against various cerebral ischaemic insults and neurodegenerative disorders. However, the function and mechanisms of LIG‐mediated hippocampal neural stem cells (H‐NSCs) activation as well as cognitive recovery in the context of post‐operative cognitive dysfunction (POCD) remain elusive and need to be explored. Mice were subjected to transient global cerebral ischaemia and reperfusion (tGCI/R) injury and treated with LIG (80 mg/kg) or vehicle for 1 month. Morris water maze test and western blot were employed to assess cognitive function. Nissl staining and immunofluorescence (IF) staining were used to detect H‐NSCs proliferation and neurogenesis in hippocampus. Subsequently, primary H‐NSCs were treated with LIG, and the level of H‐NSCs proliferation and neuronal‐differentiation was examined by IF staining for Edu and β‐Tubulin III. The protein levels of ERK1/2, β‐catenin, NICD, TLR4, Akt and FoxO1 were examined using western blotting. Finally, pretreatment with the ERK agonist SCH772984 was performed to observe the change in ERK expression. LIG treatment promoted H‐NSCs proliferation and neurogenesis, increased the number of neurons in the hippocampal subfields, and ultimately reversed cognitive impairment in tGCI/R injury. Furthermore, LIG also promoted primary H‐NSCs proliferation and neuronal‐differentiation, as well as ERK1/2 phosphorylation. Pretreatment with SCH772984 effectively reversed the ability of LIG to induce ERK1/2 phosphorylation and promote H‐NSCs proliferation and neuronal‐differentiation. LIG can promote cognitive recovery after tGCI/R injury by activating ERK1/2 in H‐NSCs to promote their proliferation and neurogenesis in the hippocampus. Therefore, LIG has potential for use in the prevention and/or treatment of POCD.

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Section: Resultsmentioning

confidence: 99%

Ligustilide enhances hippocampal neural stem cells activation to restore cognitive function in the context of postoperative cognitive dysfunction

Sun

Zhou

et al. 2021

Eur J of Neuroscience

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“…Studies have indicated that LIG may significantly improve blood circulation, protect against nerve damage and attenuate painful behavior (11,(14)(15)(16)(17). The neuroprotective effects of LIG have also been reported in several animal models of cerebral ischemia, indicating that LIG may reduce the infarct size in the ischemic area, decrease edema in the brain and improve neurobehavioral defects (18). Furthermore, LIG is thought to protect against hypoperfusion-induced nerve damage in the cerebral cortex, inhibit cortical neuron apoptosis and maintain the integrity of the neuronal structure (19).…”

Section: Introductionmentioning

confidence: 92%

Antidepressant‑like effects of Z‑ligustilide on chronic unpredictable mild stress‑induced depression in rats

Zhang

Huang

et al. 2021

Exp Ther Med

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“…In clinical practice, Ligusticum chuanxiong is widely used to treat thromboembolism heart diseases [4] , headache [5,6] , wind-damp [7] . Recent studies have found that the active metabolites of Ligusticum chuanxiong including ligustrazine [8] , ferulic acid [9] , ligustilide [10] , polysaccharide and the others [11] . Ferulic acid, a kind of phenolic acid, is one of the most important active ingredients in Ligusticum chuanxiong [12] .…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Comparison of Rhizome and Leaf of Rhizome of Ligusticum Chuanxiong on the Effects of Active Metabolites

Hao

cai

et al. 2021

Preprint

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BackgroundThe rhizome of Ligusticum chuanxiong is a well-known traditional Chinese medicine. The major bioactive compounds found in Ligusticum chuanxiong are Ferulic Acid with 4- hydroxyl -3- methoxybenzene acrylic acid. However, the epigenetic mechanisms and biosynthetic pathways of active metabolites based on the transcriptomic analysis of Ligusticum chuanxiong rhizome have remained unclear. To clarify the key genes in rhizome formation and active metabolite synthesis pathways of Rhizome of Ligusticum chuanxiong, we focus on the change between the investigate the rhizome and leaf of Ligusticum chuanxiong through transcriptomic analysis.MethodsThe RNA extracted from rhizomes and leaves of Ligusticum chuanxiong were sequenced using the Illumina platform, yielding 295,725 unique transcripts with N50 of 778 bp. The transcripts against annotation databases including GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) to identify differentially expressed genes (DEGs).Resultsystematic analysis of transcriptome profiles revealed that a total of 1707 differentially expressed unigenes were found in the two tissues, among which 1246 were down-regulated and 462 were up-regulated. Remarkably, some DEGs are enriched in the biosynthetic pathway of phenylalanine ammonia lyase, one of the key enzymes in the pathway of ferulic acid synthesis.ConclusionsThrough the transcriptome analysis, we have identified the regulatory mechanisms and epigenetic influences in the secondary metabolic biosynthetic pathway of Ligusticum chuanxiong. Among them, DEGs analysis revealed ferulic acid and other secondary metabolites are some of the major products in the biosynthetic pathway of Ligusticum chuanxiong.

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Klotho upregulation contributes to the neuroprotection of ligustilide against cerebral ischemic injury in mice

Cited by 29 publications

References 29 publications

Ligustilide enhances hippocampal neural stem cells activation to restore cognitive function in the context of postoperative cognitive dysfunction

Ligustilide enhances hippocampal neural stem cells activation to restore cognitive function in the context of postoperative cognitive dysfunction

Antidepressant‑like effects of Z‑ligustilide on chronic unpredictable mild stress‑induced depression in rats

Transcriptome Comparison of Rhizome and Leaf of Rhizome of Ligusticum Chuanxiong on the Effects of Active Metabolites

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