Klebsiella to Salmonella gene transfer within rumen protozoa: Implications for antibiotic resistance and rumen defaunation☆

McCuddin, Zoe P.; Carlson, Steve A.; Rasmussen, Mark A.; Franklin, Sharon K.

doi:10.1016/j.vetmic.2005.12.004

Cited by 53 publications

(37 citation statements)

References 35 publications

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“…enterica. Serovars of the enterica sub species can be divided into three groups depending upon their ability to infect a wide variety of hosts: Serovars which have a broad host range also called as unrestricted serovars as these infect nearly all animals and pose a greater zoonotic potential than their other counterparts (Salmonella Typhimurium and Salmonella Enteritidis) (Bäumler et al, 1998), Serovars which accidentally infect hosts other than their most adapted or preferred (Salmonella Dublin and Salmonella Choleraesuis) (McCuddin et al, 2006), and serovars which are restricted to one specific host only (Salmonella Typhi and Salmonella Gallinarum) (Uzzau et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification and antimicrobial susceptibility profiles of <i>Salmonella</i> isolates from dairy farms in and around Modjo town, Ethiopia

Abunna¹,

Ashenafi²,

Beyene³

et al. 2017

Ethiop. Vet. J.

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Foodborne bacterial diseases are a serious challenge to human and animal health. Salmonella is a zoonotic foodborne pathogen and the etiologic agent of salmonellosis. A cross-sectional study was conducted from January 2016 to April 2016 on small scale and large scale dairy farms in and around Modjo town, Ethiopia. The main objectives of the study were to isolate and identify Salmonella from lactating cows, personnel's' and equipment at farms and to determine the in vitro antimicrobial resistance profiles of the isolates. A total of 266 samples consisting of fresh cow milk, fecal sample, pooled milkers' hand swab, pooled bucket swab, tank swab, and tank milk were collected from 21 dairy farms (n=20 smallholders, n=1 large scale farm). The samples were examined for the presence of Salmonella following standard techniques and procedures outlined by the International Organization for Standardization. Kibry-Bauer disk diffusion test was used for the antimicrobial susceptibility testing. Salmonella was isolated from 28/266 (10.5%) of the total samples. Out of the 28 Salmonella isolates, 18 (64.3%), 3 (10.7%) and 7(25%) were from lactating cows, personnel's', and equipment, respectively. Out of the 28 isolates subjected to antimicrobial susceptibility testing, all isolates were resistant to at least one or more antimicrobials tested. Accordingly, 96.4% (27/28), 82.1% (23/28) and 75.0% (21/28) isolates were resistant to tetracycline, kanamycin and nalidixic acid, respectively. Multiple drug resistance (resistance to two or more antimicrobials) was detected in 27(96.4%) of the isolates. Multiple antimicrobial resistance was observed in 100% (18/18), 7.4% (2/23) and 100% (7/7) of isolates obtained from lactating cows, personnels', and equipment, respectively. High proportion of multiple antimicrobial resistant isolates (96.4%) in 93Fufa Abunna et al., Ethiop. Vet. J., 2017, 21 (2), 92-108 the dairy farms alerts concern for animal and public health as these drugs is used widely for treatment and prophylaxis in animals and humans.

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Section: Introductionmentioning

confidence: 99%

Isolation, identification and antimicrobial susceptibility profiles of <i>Salmonella</i> isolates from dairy farms in and around Modjo town, Ethiopia

Abunna¹,

Ashenafi²,

Beyene³

et al. 2017

Ethiop. Vet. J.

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“…Approximately 10 9 CFUs of both S. enterica serotype Typhimurium strain TH11 (Carlson et al, 1999) and K. pneumoniae TCR2003 (McCuddin et al, 2006) were coincubated with approximately 10 5 RPz in sealed glass tubes overnight on a rocker at 37 8C. At the end of the incubation period, extracellular Salmonella were killed using 300 mg/ml Table 1 Summary of microbes used in this study.…”

Section: Conjugation Frequency Assaymentioning

confidence: 99%

“…Salmonella enterica serotype Typhimurium DT104 strain 98-420 SGI1-bearing strain capable of exhibiting hypervirulence following exposure to RPz; florfenicol MIC equals 64 mg/ml Rasmussen et al (2005) Salmonella enterica serotype Typhimurium DT104 strain TH11 SGI1-free isostrain of DT104 incapable of hyperinvasion Carlson et al (1999) Klebsiella pneumoniae TCR2003 Isolated from turtle feces in Iowa; bears a ceftiofur resistance plasmid; ceftiofur MIC equals 64 mg/ml McCuddin et al (2006) florfenicol. RPz were then lysed using a bead beater for 60 s. The lysate was pelleted and resuspended in 200 ml Lennox L broth.…”

Section: Microbe Relevant Characteristics Referencementioning

confidence: 99%

“…Recent studies revealed that ciliated rumen protozoa (RPz), commensal microbes inhabiting the forestomach of ruminants, provide a venue for conjugal transfer where Salmonella can acquire plasmid-borne antibiotic resistance genes (McCuddin et al, 2006). Whereas many other bacteria are digested by RPz, Salmonella is an intracellular pathogen which is able to live, multiply, and accept conjugative plasmids within these microbes (McCuddin et al, 2006;Rasmussen et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Whereas many other bacteria are digested by RPz, Salmonella is an intracellular pathogen which is able to live, multiply, and accept conjugative plasmids within these microbes (McCuddin et al, 2006;Rasmussen et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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