Klebsiella pneumoniae: an increasing threat to public health

Effah, Clement Yaw; Sun, Tongwen; Liu, Shaohua; Wu, Yongjun

doi:10.1186/s12941-019-0343-8

Cited by 328 publications

(275 citation statements)

References 53 publications

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“…Another example of the opportunistic pathogens causing a broad spectrum of diseases and showing increasingly frequent acquisition of resistance to antibiotics is Klebsiella pneumoniae [ 20 ]. Hameed et al [ 90 ] tested chemically synthesized ZnO NPs doped with Nd against K. pneumoniae —the minimal inhibitory concentration was found to be 1000 μg/mL.…”

Section: Resultsmentioning

confidence: 99%

“…Currently, antibiotic resistance among pathogenic bacteria is a real problem for humanity [ 17 , 18 , 19 ]. The increasing problem of drug-resistance among many pathogens, e.g., Staphylococcus aureus , Pseudomonas aeruginosa, Klebsiella pneumonia or Escherichia coli, requires the development of novel antiseptics [ 20 , 21 , 22 , 23 ]. One of the prospective alternatives to antibiotics is, as proposed in our study, the post-cultured synthesis of zinc oxide nanocomposites by lactic acid bacteria isolated from whey.…”

Section: Introductionmentioning

confidence: 99%

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Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

et al. 2020

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This research presents, for the first time, the potential of the Lactobacillus paracasei LC20 isolated from sweet whey as a novel, effective and accessible source for post-cultured ZnO nanocomposites synthesis. The obtained nanocomposites were subjected to comprehensive characterization by a broad spectrum of instrumental techniques. Results of spectroscopic and microscopic analysis confirmed the hexagonal crystalline structure of ZnO in the nanometer size. The dispersion stability of the obtained nanocomposites was determined based on the zeta potential (ZP) measurements—the average ZP value was found to be −29.15 ± 1.05 mV in the 7–9 pH range. The ZnO nanocomposites (NCs) demonstrated thermal stability up to 130 °C based on the results of thermogravimetric TGA/DTG) analysis. The organic deposit on the nanoparticle surface was recorded by spectroscopic analysis in the infrared range (FT-IR). Results of the spectrometric study exhibited nanostructure-assisted laser desorption/ionization effects and also pointed out the presence of organic deposits and, what is more, allowed us to identify the specific amino acids and peptides present on the ZnO NCs surfaces. In this context, mass spectrometry (MS) data confirmed the nano-ZnO formation mechanism. Moreover, fluorescence data showed an increase in fluorescence signal in the presence of nanocomposites designed for potential use as, e.g., biosensors. Despite ZnO NCs’ luminescent properties, they can also act as promising antiseptic agents against clinically relevant pathogens. Therefore, a pilot study on the antibacterial activity of biologically synthesized ZnO NCs was carried out against four strains (Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa) by using MIC (minimal inhibitory concentration). Additionally, the colony forming units (CFU) assay was performed and quantified for all bacterial cells as the percentage of viable cells in comparison to a control sample (untreated culture) The nanocomposites were effective among three pathogens with MIC values in the range of 86.25–172.5 μg/mL and showed potential as a new type of, e.g., medical path or ointment formulation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

et al. 2020

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“…pneumoniae outbreaks. The emergence of K. pneumonia carrying carbapenemases and their worldwide dissemination represent significant public health crisis 26 . As carbapenem resistance bla KPC-2 and bla NDM-1 are the most widely reported among CRKP worldwide, we used genomic and phylogenetic approaches to analyze CRKP isolated from hospital in China and uncover the distribution of these two genes in global genomes from GeneBank of NCBI.…”

Section: K Pneumoniae Is Responsible For Human Infections and Patienmentioning

confidence: 99%

Prevalence and global distribution ofbla_KPC-2andbla_NDM-1genes inKlebsiella Pneumoniae

Zhang

et al. 2020

Preprint

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Carbapene-resistant Klebsiella pneumoniae infections have caused a major concern and posed a global health threat to public. bla KPC-2 and bla NDM-1 genes are the most widely reported of carbapenem resistance genes in K. pneumoniae. In this study, we investigated phylogenetic relationships of carbapene-resistant K. pneumoniae from a tertiary hospital between 2013 and 2018 in China and analyzed the global epidemiology and distribution of bla KPC-2 and bla NDM-1 gene in K.pneumoniae based on 1579 NGS genomes. We found that 19 carbapene-resistant K. pneumoniae isolated were divided into five lineages and all had high genotypic and phenotypic resistance. Two lineages (mostly ST11 and ST25) were the major type detected carrying bla KPC-2 and bla NDM-1 gene, respectively. Among global genomes data, 147 known ST types have been identified and ST11 and ST258 were the globally prevalent clones. Genetic environment analysis showed that the ISKpn27-bla KPC-2 -ISKpn6 and bla NDM-1 -ble-trpf-nagA may be the core structure in the horizontal transfer of bla KPC-2 and bla NDM-1, respectively.In addition, DNA transferase (hin) may be involved in the horizontal transfer or the expression of bla NDM-1 . This study sheds some light on the genetic environment of bla KPC-2 and bla NDM-1 and should foster further studies about the mechanism of carbapene-resistant K. pneumoniae dissemination.

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“…[2] A meta-analysis published in 2020 confirmed that the trend in the isolation rate of K. pneumoniae is increasing and there is a rapid global emergence of strains of this organism resistant to almost all antibiotics, including carbapenems. [3] The main resistance mechanism responsible for antibiotic resistance is the production of Klebsiella pneumoniae carbapenemases (KPCs), βlactamases belonging to class A. β-lactamases form an acylenzyme adduct with the antibiotic, which is then easily hydrolyzed by a water molecule; thereby, the drug is made ineffective (Scheme 1).…”

Section: Introductionmentioning

confidence: 99%

α‐Triazolylboronic Acids: A Promising Scaffold for Effective Inhibitors of KPCs

et al. 2020

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Boronic acids are known reversible covalent inhibitors of serine β‐lactamases. The selectivity and high potency of specific boronates bearing an amide side chain that mimics the β‐lactam's amide side chain have been advanced in several studies. Herein, we describe a new class of boronic acids in which the amide group is replaced by a bioisostere triazole. The boronic acids were obtained in a two‐step synthesis that relies on the solid and versatile copper‐catalyzed azide–alkyne cycloaddition (CuAAC) followed by boronate deprotection. All of the compounds show very good inhibition of the Klebsiella pneumoniae carbapenemase KPC‐2, with Ki values ranging from 1 nM to 1 μM, and most of them are able to restore cefepime activity against K. pneumoniae harboring blaKPC‐2. In particular, compound 1 e, bearing a sulfonamide substituted by a thiophene ring, proved to be an excellent KPC‐2 inhibitor (Ki=30 nM); it restored cefepime susceptibility in KPC‐Kpn cells (MIC=0.5 μg/mL) with values similar to that of vaborbactam (Ki=20 nM, MIC in KPC‐Kpn 0.5 μg/mL). Our findings suggest that α‐triazolylboronates might represent an effective scaffold for the treatment of KPC‐mediated infections.

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Klebsiella pneumoniae: an increasing threat to public health

Cited by 328 publications

References 53 publications

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

Prevalence and global distribution ofbla_KPC-2andbla_NDM-1genes inKlebsiella Pneumoniae

α‐Triazolylboronic Acids: A Promising Scaffold for Effective Inhibitors of KPCs

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Klebsiella pneumoniae: an increasing threat to public health

Cited by 328 publications

References 53 publications

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

Zinc Oxide Nanocomposites—Extracellular Synthesis, Physicochemical Characterization and Antibacterial Potential

Prevalence and global distribution ofblaKPC-2andblaNDM-1genes inKlebsiella Pneumoniae

α‐Triazolylboronic Acids: A Promising Scaffold for Effective Inhibitors of KPCs

Contact Info

Product

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Prevalence and global distribution ofbla_KPC-2andbla_NDM-1genes inKlebsiella Pneumoniae