Prevalence and global distribution ofbla_KPC-2andbla_NDM-1genes inKlebsiella Pneumoniae

Abstract

Carbapene-resistant Klebsiella pneumoniae infections have caused a major concern and posed a global health threat to public. bla KPC-2 and bla NDM-1 genes are the most widely reported of carbapenem resistance genes in K. pneumoniae. In this study, we investigated phylogenetic relationships of carbapene-resistant K. pneumoniae from a tertiary hospital between 2013 and 2018 in China and analyzed the global epidemiology and distribution of bla KPC-2 and bla NDM-1 gene in K.pneumoniae based on 1579 NGS genomes. We found that 19 carbapene-resistant K. pneumoniae isolated were divided into five lineages and all had high genotypic and phenotypic resistance. Two lineages (mostly ST11 and ST25) were the major type detected carrying bla KPC-2 and bla NDM-1 gene, respectively. Among global genomes data, 147 known ST types have been identified and ST11 and ST258 were the globally prevalent clones. Genetic environment analysis showed that the ISKpn27-bla KPC-2 -ISKpn6 and bla NDM-1 -ble-trpf-nagA may be the core structure in the horizontal transfer of bla KPC-2 and bla NDM-1, respectively.In addition, DNA transferase (hin) may be involved in the horizontal transfer or the expression of bla NDM-1 . This study sheds some light on the genetic environment of bla KPC-2 and bla NDM-1 and should foster further studies about the mechanism of carbapene-resistant K. pneumoniae dissemination.

Caracterización de aislamientos de enterobacterias doble productores de carbapenemasas en un hospital universitario

“…La emergencia de aislamientos de Kpn-KPC+NDM+ evidencia la capacidad de K. pneumoniae de producir múltiples β-lactamasas con el mismo perfil de hidrolisis. Estos aislados que habían sido descriptos previamente en diversos países (11,12,13) fueron detectados en nuestro medio en forma simultánea en un gran número de centros asistenciales. En el Hospital de Clínicas fueron detectados por primera vez en agosto de 2020, triplicándose su presencia en 2022.…”

Hypervirulent carbapenem-resistant Klebsiella pneumoniae causing highly fatal meningitis in southeastern China

“…WHO classified carbapenem-resistant K. pneumoniae (CRKP) as a critical priority tier of pathogens and the highest priority in novel antimicrobial development in 2017 (8). In China, KPC is the most prevalent genetic mechanism of carbapenem resistance in K. pneumoniae species, and it is frequently coupled with other forms of β-lactamases (e.g., ESBLs) (7,9,28,29). In this study, we discovered the co-existence of KPC gene and ESBLs genes.…”

Risk Factors and Molecular Mechanism of Polymyxin B Resistance in Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Fujian, China

“… 6 Among these, bla KPC-2 and bla NDM-1 are the most common carbapenem enzyme genes that cause outbreaks in China. 7 The bla KPC gene was first discovered in the United States in 1996, 8 it had a long history and was highly contagious. According to the China Antimicrobial Surveillance Network (CHINET), the resistance rate of K. pneumoniae to carbapenems increased rapidly from 2.9% in 2005 to more than 25.3% in 2019, 9 which means that the resistance mechanism of K. pneumoniae to carbapenems should be given serious attention.…”

“…6 Among these, bla KPC-2 and bla NDM-1 are the most common carbapenem enzyme genes that cause outbreaks in China. 7 The bla KPC gene was first discovered in the United States in 1996, 8 it had a long history and tobramycin, gentamicin, ciprofloxacin, levofloxacin, and nitrofurantoin. Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 were used as quality control standards.…”