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Abstract

The influence of culture method (free-floating cells in liquid nutrient broth or bacteria attached to agar surface on solid agarized medium of the same formulation) and bacterial age on the composition of free lipids in Yersinia pseudotuberculosis (O:Ib serovar, strain KS 3058) grown in the cold (5 degrees C) has been investigated. The specific growth rate of the bacteria on solid medium was about threefold less than that in liquid medium. The qualitative composition of phospholipids and fatty acids only slightly depended on the bacterial culture method. At the same time, the colonially growing cultures contained somewhat more total lipids, they synthesized more phospholipids, in the linear growth phase they contained more lysophosphatides, and they had higher fatty acid unsaturation index and higher pathogenic potential than their "planktonic" counterparts grown in otherwise identical conditions. The bacterial growth phase influenced the amount of 3-hydroxytetradecanoic acid and, indirectly, that of lipopolysaccharide. The dynamics of changes in the amount of this acid with bacterial age was opposite in the surface and broth cultures.

10.1007/s10541-008-2004-y

“…Earlier we showed [12] that the qualitative composi tion of phospholipids and fatty acids of pseudotuberculo sis bacteria only slightly depends upon cultivation approach. However, the quantity of free lipids and relative content of phospholipids and neutral lipids (NL) in lipid extracts of Y. pseudotuberculosis varied with culture age and growth medium used (on nutrient broth (NB) or nutrient agar (NA)) [12].…”

“…This tendency was clearest for changes in the lysophosphatidylethanolamine (LPE) content. Adherent bacteria in linear and early stationary phase had an anomaly high level of LPE (35.9 and 18.2%, respec tively) compared to free living microorganisms (6.1 and 5.5%, respectively) [12].…”

“…However, the quantity of free lipids and relative content of phospholipids and neutral lipids (NL) in lipid extracts of Y. pseudotuberculosis varied with culture age and growth medium used (on nutrient broth (NB) or nutrient agar (NA)) [12]. Phospholipid content changed and the character of its alteration during cell aging showed different tendencies for suspension and colonial cultures.…”

Optimization of the Conditions for Cultivation of Yersinia pseudotuberculosis in the Process of Obtaining Cell Mass

“…внесение в питательную среду ряда веществ, в частности углеродного субстрата, также позволяет увеличить выход клеточной массы. так, согласно результатам ряда авторов, использование галактозы при культивировании штаммов Y. pseudotuberculosis способствует активному приросту клеточной массы [6]. исходя из вышесказанного, актуальным является подбор оптимальных сред и условий культивирования с целью увеличения выхода клеточной массы Y. pseudotuberculosis.…”