The impact of abiotic factors (temperature and glucose) on physicochemical properties of lipids from Yersinia pseudotuberculosis

Бахолдина, С. И.; Sanina, N. M.; Красикова, И. Н.; Popova, Olga; Соловьева, Т. Ф.

doi:10.1016/j.biochi.2004.10.011

Cited by 22 publications

(16 citation statements)

References 34 publications

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“…A number of related observations have been reported previously. Besides alteration of the fatty acid composition, adaptation to temperature also results in changes in saturation and branching (4,37,54). Bacillus cereus strains respond to a decrease in growth temperature from 37 to 15°C with an increase in the ratio of anteiso-branched fatty acids to iso-branched fatty acids, as well as in the ratio of unsaturated fatty acids to saturated fatty acids (24).…”

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confidence: 99%

Osmolality, Temperature, and Membrane Lipid Composition Modulate the Activity of Betaine Transporter BetP in Corynebacterium glutamicum

et al. 2007

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The gram-positive soil bacterium Corynebacterium glutamicum, a major amino acid-producing microorganism in biotechnology, is equipped with several osmoregulated uptake systems for compatible solutes, which is relevant for the physiological response to osmotic stress. The most significant carrier, BetP, is instantly activated in response to an increasing cytoplasmic K ؉ concentration. Importantly, it is also activated by chill stress independent of osmotic stress. We show that the activation of BetP by both osmotic stress and chill stress is altered in C. glutamicum cells grown at and adapted to low temperatures. BetP from cold-adapted cells is less sensitive to osmotic stress. In order to become susceptible for chill activation, cold-adapted cells in addition needed a certain amount of osmotic stimulation, indicating that there is cross talk of these two types of stimuli at the level of BetP activity. We further correlated the change in BetP regulation properties in cells grown at different temperatures to changes in the lipid composition of the plasma membrane. For this purpose, the glycerophospholipidome of C. glutamicum grown at different temperatures was analyzed by mass spectrometry using quantitative multiple precursor ion scanning. The molecular composition of glycerophospholipids was strongly affected by the growth temperature. The modulating influence of membrane lipid composition on BetP function was further corroborated by studying the influence of artificial modulation of membrane dynamics by local anesthetics and the lack of a possible influence of internally accumulated betaine on BetP activity.

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Osmolality, Temperature, and Membrane Lipid Composition Modulate the Activity of Betaine Transporter BetP in Corynebacterium glutamicum

et al. 2007

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“…At the low LPE level in cells growing in glucose-free medium, PBEH had no effect on the MIC of ampicillin acting on Y. pseudotuberculosis cells. So, PBEH could promote the antibiotic effect on bacterial cells due to inhibition of phospholipase A, whose activity, on the contrary, is strengthened in glucose-grown cells [Bakholdina et al, 2004]. However, recent computational studies have revealed yet another possible mechanism of action of plant polyphenols, leading to a decrease in lysophospholipid production.…”

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confidence: 99%

“…As an ectothermic organism, Y. pseudotuberculosis changes its own lipid profile depending on the cultivation conditions. In particular, the availability of glucose in nutrition broth, increased temperature, anaerobic conditions, or phenol biocide result in the accumulation of a remarkable amount of lysophosphatidylethanolamine (LPE) mainly in the OM [Bakholdina et al, 2004;Sanina et al, 2013;Davydova et al, 2016]. These adaptive changes significantly influence the conformation of the major OM protein OmpF that may hinder transport of the various lowmolecular substances, including β-lactam antibiotics, through the OM [Sanina et al, 2013].…”

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confidence: 99%

“…To manipulate the level of LPE in membranes of Y. pseudotuberculosis, we used the effect of the glucose added to the cultural medium that stimulate the formation of LPE [Bakholdina et al, 2004], and polyphenol extract from buckwheat husks (PEBH), which can inhibit phospholipase A [Fei et al, 2018] and, accordingly, reduce the content of LPE in bacterial cells.…”

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confidence: 99%

“…Phospholipids were analyzed by thin-layer chromatography on silica gel plates as previously described [Bakholdina et al, 2004]. PBEH was prepared by the original method [Reutov et al, 2018].…”

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Relationship between Adaptive Changing of Lysophosphatidylethanolamine Content in the Bacterial Envelope and Ampicillin Sensitivity of <b><i>Yersinia pseudotuberculosis</i></b>

et al. 2018

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The low permeability of porin channels is the possible reason for Gram-negative bacterial resistance to antibiotics. The adaptive accumulation of lysophosphatidylethanolamine (LPE) in Yersinia pseudotuberculosis induces conformational changes of OmpF porin that may hinder the transport of antibiotics through this channel. The present study was aimed to test whether the changes in LPE content affect the resistance of bacteria to ampicillin. The addition of glucose to the culture medium was shown to simultaneously increase the level of LPE and minimum inhibitory concentration (MIC) for ampicillin of Y. pseudotuberculosis cells 6- and 2-fold, respectively. However, the coadministration of glucose and polyphenol extract from buckwheat husks reduced the content of LPE 2-fold and restored MIC to the control value. Thus, PBEH can be used as antibiotic adjuvant to improve an antibiotic’s ability to cross the outer membrane. The present work demonstrated: (i) the role of adaptive changes in the lipid composition of Y. pseudotuberculosis in the development of antibiotic resistance, and (ii) the promising use of PBEH in combination therapy to increase the susceptibility of Gram-negative bacteria to the conventional β-lactam antibiotics, probably attenuating in vivo a previously demonstrated effect of LPE on the conformation and function of the OmpF channel.

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Competition between cis, trans and Cyclopropane Fatty Acid Formation and its Impact on Membrane Fluidity

Loffhagen¹,

Härtig²,

Geyer³

et al. 2007

Engineering in Life Sciences

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The impact of cis, trans and cyclopropane fatty acids on membrane fluidity was investigated using batch‐grown Pseudomonas putida P8 and Comamonas testosteroni ATCC 17454. A major difference observed between the two investigated strains is the absence of the ability to synthesize trans‐unsaturated fatty acids in Comamonas. When grown exponentially at 30 °C, a shift to 35 °C increased the trans/cis ratios of the fatty acids of P. putida P8 from 0 to 0.81 and 0 to 1.07, in lipid extracts and cell hydrolyzates, respectively. After prolonged growth followed by nutrient deprivation for 48 h, both at 30 °C, trans fatty acids were absent, but the cyclo/cis ratios rose from 0.1 to 1.55 in lipid extracts, and from 0.1 to 1.54 in cell hydrolyzates. C. testosteroni ATCC 17454 contained no cyclo fatty acids when harvested in the exponential phase after 6 h, whereas after 72 h cultivation, the cyclo/cis ratios rose to 0.49 and 0.47, in lipid extracts and cell hydrolyzates, respectively. Trans fatty acids were never observed in this strain. Increased cyclo/cis and trans/cis ratios correlated with decreased fluidity measured by the fluorescence anisotropy of 1,6‐diphenyl‐1,3,5‐hexatriene (DPH) intercalated in the bilayers of liposomes and by Fourier Transform Infrared (FTIR) spectroscopy of lipids prepared from the cells. The specific effect of cyclopropane fatty acids on membrane fluidity was much smaller than that of trans fatty acids. FTIR‐measurements of intact cells of P. putida P8 confirmed the high potency of trans fatty acids to decrease the fluidity. In cells with induced cyclopropane fatty acid synthesis, the membranes remained more fluidized, indicating the lower importance of these fatty acids for homeoviscosis.

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The impact of abiotic factors (temperature and glucose) on physicochemical properties of lipids from Yersinia pseudotuberculosis

Cited by 22 publications

References 34 publications

Osmolality, Temperature, and Membrane Lipid Composition Modulate the Activity of Betaine Transporter BetP in Corynebacterium glutamicum

Osmolality, Temperature, and Membrane Lipid Composition Modulate the Activity of Betaine Transporter BetP in Corynebacterium glutamicum

Relationship between Adaptive Changing of Lysophosphatidylethanolamine Content in the Bacterial Envelope and Ampicillin Sensitivity of <b><i>Yersinia pseudotuberculosis</i></b>

Competition between cis, trans and Cyclopropane Fatty Acid Formation and its Impact on Membrane Fluidity

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