Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA

Drasbek, Mette; Nielsen, Peter Kresten; Persson, Kenneth; Birkelund, Svend; Christiansen, Gunna

doi:10.1186/1471-2180-4-7

Cited by 25 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ELISA for Campylobacter jejuni , Salmonella enteritidis and human HSP60 were performed as described [22]. ELISA plates were coated with 4 µg/ml human HSP60, C. jejuni HSP60 or S. enteritidis HSP60.…”

Section: Methodsmentioning

confidence: 99%

Increased Levels of IgG Antibodies against Human HSP60 in Patients with Spondyloarthritis

et al. 2013

Self Cite

View full text Add to dashboard Cite

Spondyloarthritis (SpA) comprises a heterogeneous group of inflammatory diseases, with strong association to human leukocyte antigen (HLA)-B27. A triggering bacterial infection has been considered as the cause of SpA, and bacterial heat shock protein (HSP) seems to be a strong T cell antigen. Since bacterial and human HSP60, also named HSPD1, are highly homologous, cross-reactivity has been suggested in disease initiation. In this study, levels of antibodies against bacterial and human HSP60 were analysed in SpA patients and healthy controls, and the association between such antibodies and disease severity in relation to HLA-B27 was evaluated.Serum samples from 82 patients and 50 controls were analysed by enzyme-linked immunosorbent assay (ELISA) for immunoglobulin (Ig)G1, IgG2, IgG3 and IgG4 antibodies against human HSP60 and HSP60 from Chlamydia trachomatis, Salmonella enteritidis and Campylobacter jejuni. Disease severity was assessed by the clinical scorings Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), Bath Ankylosing Spondylitis Functional Index (BASFI) and Bath Ankylosing Spondylitis Metrology Index (BASMI).Levels of IgG1 and IgG3 antibodies against human HSP60, but not antibodies against bacterial HSP60, were elevated in the SpA group compared with the control group. Association between IgG3 antibodies against human HSP60 and BASMI was shown in HLA-B27+ patients. Only weak correlation between antibodies against bacterial and human HSP60 was seen, and there was no indication of cross-reaction.These results suggest that antibodies against human HSP60 is associated with SpA, however, the theory that antibodies against human HSP60 is a specific part of the aetiology, through cross-reaction to bacterial HSP60, cannot be supported by results from this study. We suggest that the association between elevated levels of antibodies against human HSP60 and disease may reflect a general activation of the immune system and an increased expression of human HSP60 in the synovium of patients with SpA.

show abstract

Section: Methodsmentioning

confidence: 99%

Increased Levels of IgG Antibodies against Human HSP60 in Patients with Spondyloarthritis

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Prior to the advent of antibiotics and vaccination, it was a leading cause of mortality ( 1 , 5 ). At present, the most common bacterial causes of pneumonia are the so-called atypical bacteria, Mycoplasma pneumoniae and Chlamydophila pneumonia ( 7 ). Legionella pneumophila is also considered atypical; however, it is less common ( 1 , 2 , 6 ).…”

Section: Discussionmentioning

confidence: 99%

“…The pathogenic process of all types of pneumonia is able to cause acute or chronic inflammation in the mediastinal lymph nodes, lymph node hyperemia edema, hyperplasia of lymphocytes and macrophages, and infiltration of neutrophils, monocytes, and plasma cells ( 7 , 10 ). In addition, the lymph nodes may experience necrosis and granuloma formation ( 7 , 11 ).…”

Section: Discussionmentioning

confidence: 99%

Adult community-acquired pneumonia with unusually enlarged mediastinal lymph nodes: A case report

Zhang

Qiu

Tang

et al. 2017

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

Adult community-acquired pneumonia (ACAP) is the most prevalent pulmonary infectious disease that may be asymptomatic or have varying clinical presentations. Patients with ACAP often present with enlarged mediastinal lymph nodes on their chest computed tomography images. However, large irregular swollen lymph nodes are rarely reported in ACAP, and may therefore be confused with enlarged lymph node masses. In the present case report, the patient presented with lymph node masses, which were ameliorated to their normal size following antimicrobial treatment. The patient was 24 years old and otherwise healthy, which led to a pronounced and excessive immune response to pneumonia in the lymph nodes. Atypical pneumonia is difficult to diagnose based on imaging features. The present case report demonstrates that patients with pneumonia may present with unusually enlarged mediastinal lymph nodes, which are most likely, a result of a strong immune response to pneumonia.

show abstract

“…Antigenic properties of recombinant proteins P116 and P30 have also been shown [15,18,19]. A combination of frequently recognized antigens could be useful for diagnostic purposes.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, many studies have reported great interest in using a recombinant protein corresponding to the C-terminal portion of the P1 adhesin, which has been described as the immunodominant antigen in M. pneumoniae [ 2 , 13 - 17 ]. Antigenic properties of recombinant proteins P116 and P30 have also been shown [ 15 , 18 , 19 ]. A combination of frequently recognized antigens could be useful for diagnostic purposes.…”

Section: Introductionmentioning

confidence: 99%

Identification, expression and serological evaluation of the recombinant ATP synthase beta subunit of Mycoplasma pneumoniae

et al. 2010

View full text Add to dashboard Cite

BackgroundMycoplasma pneumoniae is responsible for acute respiratory tract infections (RTIs) common in children and young adults. As M. pneumoniae is innately resistant to β-lactams antibiotics usually given as the first-line treatment for RTIs, specific and early diagnosis is important in order to select the right treatment. Serology is the most used diagnostic method for M. pneumoniae infections.ResultsIn this study, we identified the M. pneumoniae ATP synthase beta subunit (AtpD) by serologic proteome analysis and evaluated its usefulness in the development of a serological assay. We successfully expressed and purified recombinant AtpD (rAtpD) protein, which was recognised by serum samples from M. pneumoniae-infected patient in immunoblots. The performance of the recombinant protein rAtpD was studied using a panel of serum samples from 103 infected patients and 86 healthy blood donors in an in-house IgM, IgA and IgG enzyme-linked immunosorbent assay (ELISA). The results of this assay were then compared with those of an in-house ELISA with a recombinant C-terminal fragment of the P1 adhesin (rP1-C) and of the commercial Ani Labsystems ELISA kit using an adhesin P1-enriched whole-cell extract. Performances of the rAtpD and rP1-C antigen combination were further assessed by binary logistic regression analysis. We showed that combination of rAtpD and rP1-C discriminated maximally between the patients infected with M. pneumoniae (children and adults) and the healthy subjects for the IgM class, performing better than the single recombinant antigens or the commercial whole-cell extract.ConclusionThese results suggest that AtpD can be used as an antigen for the immunodiagnosis of early and acute M. pneumoniae infection in association with adhesin P1, providing an excellent starting point for the development of point-of-care diagnostic assays.

show abstract

Immune response to Mycoplasma pneumoniae P1 and P116 in patients with atypical pneumonia analyzed by ELISA

Cited by 25 publications

References 26 publications

Increased Levels of IgG Antibodies against Human HSP60 in Patients with Spondyloarthritis

Increased Levels of IgG Antibodies against Human HSP60 in Patients with Spondyloarthritis

Adult community-acquired pneumonia with unusually enlarged mediastinal lymph nodes: A case report

Identification, expression and serological evaluation of the recombinant ATP synthase beta subunit of Mycoplasma pneumoniae

Contact Info

Product

Resources

About