Kinetin Inhibits Protein Oxidation and Glycoxidation in Vitro

Journal of Plant Interactions

Dey

et al. 2014

Salvinia natans L. response to hydrogen peroxide (H 2 O 2 ) induced oxidative stress through physiological activities was evaluated. The plants were incubated with varying concentrations (0, 50, 100 µM) of H 2 O 2 and 100 µM of H 2 O 2 supplemented with 1 mM putrescine (Put) in hydroponic culture. This is observed with the decline in proline content and its biosynthetic enzymes viz. γ-glutamyl kinase and γ-glutamyl phosphate reductase activity. Protein carbamylated derivative by protein oxidation was another trait for oxidative damages by H 2 O 2 . The antioxidative enzymes like guaiacol peroxidase (GPX), glutathione reductase (GR), and catalase (CAT) recorded to express through in-gel staining with the H 2 O 2 exposure. On nuclear level, plants were sensitive to H 2 O 2 where the DNA disintegration was studied with comet assay and maximum comet tail observed at 100 µM H 2 O 2 treatment. Application of Put reduced the generation of protein oxidation and comet tail length as well as moderated the enzyme activity as revealed through in-gel staining.

Section: Determination Of Protein Oxidationmentioning

confidence: 99%

Effects of putrescine on oxidative stress induced by hydrogen peroxide in Salvinia natans L.

Journal of Plant Interactions

Dey

et al. 2014

“…It was measured by the reaction with di-nitro-phenyl-hydrazine (DNPH) (Verbeke et al, 2000). The leaf homogenate was extracted with 6% (w/v) SDS followed by 25 minutes incubation at room temperature (4°C).…”

Section: Protein Oxidation Assaymentioning

confidence: 99%

Responses of the maize plant to chromium stress with reference to antioxidation activity

Maiti

Braz. J. Plant Physiol.

et al. 2012

Two maize varieties, viz. Sartaj and Deccan, have been evaluated according to their responses to chromium (Cr) in terms of specific physiological and biochemical attributes. During the exposure to Cr concentration (0, 50, 100, 200 and 300 µM) of potassium dichromate (K 2 Cr 2 O 7 ) for seven days, significant changes of oxidative stress in terms of lipid peroxidation and protein oxidation were recorded along with other antioxidative pathways. It is revealed that irrespective of varieties, Sartaj was more prone to oxidative stress since the amount of superoxide due to Cr exposure was found to be significant, unlike the second variety, Deccan. In addition, for Sartaj the activity of NAD(P)H oxidase recorded a significant linear increase in a dose response manner.The changes of antioxidative enzymes recorded significant variation in both varieties under stress compared to control. However, Deccan maintains a consistent up regulation in SOD and GPX activities. Contrarily, APX activity was less sensitive to both varieties. The activation of antioxidative mechanisms due to the impact of Cr-induced oxidative stress has been described subsequently. The results deriving from this investigation might be effective for the selection of tolerant plant types under chromium contamination.

“…Protein oxidation assay was conducted by dinitrophenyl hydragene (DNPH) reagent according to Verbeke et al (2000). 1.0 g sample was thoroughly crushed in 6 % (w/v) sodium lauryl sulphate (SDS) followed by incubation at 37°C for 30 min.…”

Section: Plant Materialsmentioning

confidence: 99%

Antioxidative responses of Salvinia (Salvinia natans Linn.) to aluminium stress and it’s modulation by polyamine

Physiol Mol Biol Plants

Maiti

et al. 2012

Antioxidative stress response of free-floating aquatic fern (Salvinia natans Linn.) was studied under increasing toxic amount of aluminium (Al) and its modulation by exogenous application of polymaine. Increased levels of superoxide (O 2 − ) and hydrogen peroxide (H 2 O 2 ) species from affected tissues suggested that plants were undergoing oxidative stress and it was concominant with increased accumulation of Al in a dose dependent manner. Application of polyamine like putrescine (Put) led to a decrease in oxidative stress as revealed by reduced level of O 2 − and H 2 O 2 . Al toxicity resulted into decreased biomass that was ameliorated by the application of Put. The changes observed in lipid peroxidation (MDA) and protein oxidation also indicated that plats are undergoing Al induced oxidative stress. In order to circumvent the oxidative stress resulting from Al toxicity, plants enzymatic and nonenzymatic antioxidant pathways were active. The ratio of both oxidized and reduced cellular glutathione exhibited significant variation in response to Al stress and was improved upon Put treatment. Peroxidase and glutathione were upregulated whereas catalse was downregulated under varying doses of Al. Isozyme profile of above enzymes also showed a trend with increasing amount of Al. The nuclear disintegration study using comet assay was indicative of Al induced oxidative stress. In the present study, we have explored the antioxidative response of aquatic fern Salvinia natans Linn in response to Al toxicity. The application of polyamine Put improved the overall antioxidative response and thus would make it a better candidate to be used as hyper accumulator of Al and other toxic metals.