Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats

Li, Mengyun; Ouyang, Wei; Wu, Xiaoli; Zheng, Yin; Wei, Yunpeng; An, Liguo

doi:10.4142/jvs.2014.15.3.353

Cited by 13 publications

(5 citation statements)

References 19 publications

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“…Our results are in agreement with Li M et.al [29] who showed that kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats and with Ratten et.al [30] who reported that kinetin protects against Fenton reaction-mediated oxidative damage to DNA. Verbeke et al [31] also reported the protective action of kinetin against protein oxidation and glycoxidation in vitro .…”

Section: Resultssupporting

confidence: 93%

The Plant Hormone Cytokinin Confers Protection against Oxidative Stress in Mammalian Cells

et al. 2016

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Modulating key dynamics of plant growth and development, the effects of the plant hormone cytokinin on animal cells gained much attention recently. Most previous studies on cytokinin effects on mammalian cells have been conducted with elevated cytokinin concentration (in the μM range). However, to examine physiologically relevant dose effects of cytokinins on animal cells, we systematically analyzed the impact of kinetin in cultured cells at low and high concentrations (1nM-10μM) and examined cytotoxic and genotoxic conditions. We furthermore measured the intrinsic antioxidant activity of kinetin in a cell-free system using the Ferric Reducing Antioxidant Power assay and in cells using the dihydroethidium staining method. Monitoring viability, we looked at kinetin effects in mammalian cells such as HL60 cells, HaCaT human keratinocyte cells, NRK rat epithelial kidney cells and human peripheral lymphocytes. Kinetin manifests no antioxidant activity in the cell free system and high doses of kinetin (500 nM and higher) reduce cell viability and mediate DNA damage in vitro. In contrast, low doses (concentrations up to 100 nM) of kinetin confer protection in cells against oxidative stress. Moreover, our results show that pretreatment of the cells with kinetin significantly reduces 4-nitroquinoline 1-oxide mediated reactive oxygen species production. Also, pretreatment with kinetin retains cellular GSH levels when they are also treated with the GSH-depleting agent patulin. Our results explicitly show that low kinetin doses reduce apoptosis and protect cells from oxidative stress mediated cell death. Future studies on the interaction between cytokinins and human cellular pathway targets will be intriguing.

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Section: Resultssupporting

confidence: 93%

The Plant Hormone Cytokinin Confers Protection against Oxidative Stress in Mammalian Cells

et al. 2016

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“…Aging-related decline or aging-associated pathological conditions contain many aspects including oxidative stress damage, chronic low-level inflammation, destructed cells and tissue lining and dysfunction of organs. Studies have showed that a significant increased level of MDA and decreased level of SOD activity and GSH-Px activity in D-galactose induced aging mice ( 30 ) and D-galactose induced aging rats ( 31 ). Our data revealed similar results in D-galactose induced aging dogs, compared with young control and naturally aging dogs, suggesting that aging dogs have more oxidative stress and free radical but less anti-oxidant and enzymes to handle these stress resulting in serious damage in tissue and organs.…”

Section: Discussionmentioning

confidence: 99%

Comparison of naturally aging and D‑galactose induced aging model in beagle dogs

Liu

et al. 2017

Exp Ther Med

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Animal models have been used to study aging for decades. In numerous aging studies, beagles are the most commonly used breed of dog. However, few studies have compared between naturally aging models and experimentally induced aging models in beagle dogs. In the present study, a D-galactose induced aging model was compared with a naturally aging model, and young adult dogs were considered as the young control group. The level of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in serum, and brain tissue were measured. Histopathological comparisons of the liver, kidneys, heart, lungs and spleen were evaluated using hematoxylin and eosin (H&E) staining, in addition, the brain was evaluated by H&E staining, and Nissl staining. The expression levels of aging-associated factors in the hippocampus, including proliferating cell nuclear antigen (PCNA), P16 and P21 were also determined through reverse transcription quantitative-polymerase chain reaction, and western blot analysis. The results indicated that D-galactose induced aging significantly increased the MDA level, while the levels of SOD and GSH-Px were diminished when compared with the young control group, which was similar to the naturally aging group. Parallel histopathological features were observed in the D-galactose induced aging and naturally aging groups compared with the young control group. However, a reduced expression level of PCNA, and increased expression levels of P16 and P21 were observed in the naturally ageing and induced aging groups compared with the young control group. The results of the current study demonstrated that the beagle dogs in D-galactose induced aging model exhibited significant similarities with the naturally aging model, providing evidence to support that the D-galactose induced aging model may be applied to aging studies.

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“…During the aging process, IL-2 levels were found to be decreased while IL-6 production increase. [ 1 ]…”

Section: Discussionmentioning

confidence: 99%

“…Aging is a spontaneous physiological inevitable process,[ 1 ] so, more attention was given to studies investigating age-dependent changes in body tissues. All body organs lose function as we age.…”

Section: Introductionmentioning

confidence: 99%

Can intranasal administration of adipose-derived stem cells reach and affect the histological structure of distant organs of aged wistar rat?

Hamam¹,

Bahaa²,

Raafat³

2023

J Microsc Ultrastruct

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Introduction: Stem cell therapy is a highly promising strategy in various degenerative diseases. Intranasal administration of stem cells could be considered as a non-invasive treatment option. However, there is great debate concerning the ability of stem cells to reach distant organs. It is also unclear in such a case if they can alleviate age-related structural changes in these organs. Aim: The aim of this study is to evaluate the ability of intranasal administration of adipose-derived stem cells (ADSCs) to reach distant organs of rats at different time intervals and to investigate their effects on age-related structural changes in these organs. Materials and Methods: Forty-nine female Wistar rats were used in this study, seven of which were adults (6-month-old) and 42 were aged (2-year-old). Rats were divided into three-groups: Group-I (adult control), Group-II (aged), and Group-III (aged ADSCs treated). Rats of Groups I and II were sacrificed after 15 days from the beginning of the experiment. Rats of Group III were treated with intranasal ADSCs and were sacrificed after 2-h, 1-day, 3-day, 5-day, and 15-day. Heart, liver, kidney, and spleen specimens were collected and processed for H and E, CD105 immunohistochemistry, and immunofluorescent techniques. Morphometric study and statistical analysis were performed. Results: ADSCs appeared in all organs examined after 2-h of intranasal administration. Their maximum presence was detected after 3-day of administration, after which their immunofluorescence gradually decreased and nearly disappeared from these organs by the 15 th day. Improvement of some age-related deterioration in the structure of the kidney and liver occurred at day 5 after intranasal administration. Conclusions: ADSCs effectively reached the heart, liver, kidney, and spleen after intranasal administration. ADSCs ameliorated some age-related changes in these organs.

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Kinetin inhibits apoptosis of aging spleen cells induced by D-galactose in rats

Cited by 13 publications

References 19 publications

The Plant Hormone Cytokinin Confers Protection against Oxidative Stress in Mammalian Cells

The Plant Hormone Cytokinin Confers Protection against Oxidative Stress in Mammalian Cells

Comparison of naturally aging and D‑galactose induced aging model in beagle dogs

Can intranasal administration of adipose-derived stem cells reach and affect the histological structure of distant organs of aged wistar rat?

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